[1]甄 辉,杨冠东,杜少平,等.医用空气过滤装置对粉尘螨杀灭的实验研究[J].江西师范大学学报(自然科学版),2017,(03):238-241.
 ZENG Hui,YANG Guandong,DU Shaoping,et al.The Experimental Study on Acaricidal Activity of Medical Air Purification Device Against Dermatophagoides farinae[J].Journal of Jiangxi Normal University:Natural Science Edition,2017,(03):238-241.
点击复制

医用空气过滤装置对粉尘螨杀灭的实验研究()
分享到:

《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年03期
页码:
238-241
栏目:
出版日期:
2017-05-01

文章信息/Info

Title:
The Experimental Study on Acaricidal Activity of Medical Air Purification Device Against Dermatophagoides farinae
作者:
甄 辉杨冠东杜少平肖小军刘志刚
1.浙江省医疗器械研究所,浙江 杭州 310009; 2.广州工业微生物检测中心,广东 广州 51066; 3.深圳大学医学院过敏反应与免疫学研究所,广东 深圳 518060
Author(s):
ZENG HuiYANG GuandongDU ShaopingXIAO XiaojunLIU Zhigang
1.Zhejiang Medical Institute Devices,Hangzhou Zhejiang 310009,China; 2.Guangzhou Industry Microbe Test Center,Guangzhou Guangdong 51066,China; 3.Allergy and Immunology Institute,School of Medicine,Shenzhen University,Shenzhen Guangdong 518060,China
关键词:
医用空气过滤装置 粉尘螨 杀螨 过敏原 Der f
Keywords:
medical air purification device dust mites acaricidal activity allergens Der f
分类号:
R 392.13
文献标志码:
A
摘要:
为探讨医用空气过滤装置对粉尘螨杀灭效果及降低空气中尘螨过敏原含量的影响,挑取粉尘螨成虫放置在空气过滤装置中,按不同时间段(0,12,24,48,72,96 h)分组,每组200只粉尘螨,处理后采取样本进行杀螨率检测及扫描电镜观察; 采用抗原雾化法检测,收集空气过滤装置处理的1 h样品,并通过双抗体夹心法检测样品中抗原含量.结果表明:空气净化装置处理后尘螨死亡率随着作用时间的延长而逐渐升高,在72 h和96 h,尘螨死亡率分别达到60.5%和92.5%; 处理后的死螨严重脱水,虫体形态高度皱缩.医用空气过滤装置对空气中粉尘螨主要抗原Der f 1和Der f 2具有显著降低作用.
Abstract:
To observe the acaricidal activity of medical air purification device against dust mites and to detect the indoor allergen concentration before and after the air purification device operate,adult dust mites are placed in the air purifier and divided into six groups according to different time points(0,12,24,48,72 and 96 hours),with 200 dust mites in each group.The death of dust mites is counted and observed under scanning electron microscope(SEM).The concentrations of dust mite allergens of which are treated by the air purifier in different timing points were detected by ELISA.The results show that the death rate rise with increasing over time of the air purifier opening.The death rate of dust mites at 72 h and 96 h are respectively 60.5% and 92.5%.Scanning electron microscopy clearly show the dead dust mites showed dry,flat and wrinkled body,curled limbs,significant changes in shapes.The medical air purification device can kill dust mites by creating a relatively dry environment and also reduce the concentrations of dust mite allergens Der f1 and Der f2.

参考文献/References:

[1] 肖小军,张慧云,林瑛姿,等.1,25(OH)_2VD_3在过敏原特异性免疫治疗花粉过敏哮喘中的应用 [J].江西师范大学学报:自然科学版,2016,40(2):186-189.
[2] 幸鹏,刘玉琳,喻海琼,等.粉尘螨过敏原脂肪酶的表达、纯化及生物信息学分析 [J].江西师范大学学报:自然科学版,2015,39(1):101-105.
[3] Bousquet J,Lockey R,Malling H J.Allergen immunotherapy:therapeutic vaccines for allergic diseases [J].J Allergy Clin Immunol,1998,102(4):558-562.
[4] Bush P K,Portnoy J M.The role and abatement of fungal allergens in allergic diseases [J].J Allergy Clin Immunol,2001,107(3 Suppl):430-440.
[5] Pedersen S E,Hurd S S,Lemanske R F Jr,et al.Global strategy for the diagnosis and management of asthma in children 5 years and younger [J].Pediatr Pulmonol,2011,46(1):1-17.
[6] 刘晓宇,吴捷,王斌,等.中国不同地理区域室内尘螨的调查研究 [J].中国人兽共患病学报,2010,26(4):310-314.
[7] Hill D J,Thompson P J,Stewart G A,et al.The melbourne house dust mite study:Elimination house dust mites in the domestic environment [J].J Allergy Clin Immunol,1997,99(3):323-329.
[8] 刘晓宇,马忠校,赵莹颖,等.粉尘螨在空气净化器作用下扫描电镜形态观察 [J].南昌大学学报:医学版,2013,53(2):6-9.
[9] 马忠校,刘晓宇,杨小猛,等.空气净化器降低室内尘螨过敏原含量及其免疫反应性的实验研究 [J].中国人兽共患病学报,2013,29(2):133-137.
[10] Evans R.Environmental control and immunotherapy for allergic disease [J].J Allergy Clin Immunol,1992,90(3):462-468.
[11] 练玉银,杨杏芬.尘螨变应原含量检测研究进展 [J].热带医学杂志,2006,6(5):603-605.
[12] Baena-Cagnani C E,Gómez R M,Baena-Cagnani R,et al.Impact of environmental tobacco smoke and active tobacco smoking on the development and outcomes of asthma and rhinitis [J].Curr Opin Allergy Clin Immunol,2009,9(2):136-140.
[13] Agrawal S R1,Kim H J,Lee Y W,et al.Effect of an air cleaner with electrostatic filter on the removal of airborne house dust mite allergens [J].Yonsei Med J,2010,51(6):918-923.

备注/Memo

备注/Memo:
收稿日期:2015-11-16基金项目:国家自然科学基金(91442118,31400786),杭州市“115”引进国外智力计划、高层次留学回国人员(团队)在杭创业创新基金和杭州高新开发区(滨江)5050计划资助项目.作者简介:甄 辉(1966-),男,安徽滁州人,高级工程师,主要从事光机电一体化设备研究.E-mail:zhenhui@zmdcc.com
更新日期/Last Update: 1900-01-01