[1]简敏菲,王童童,余厚平,等.河岸带植物羊蹄在2种去叶胁迫下的生长及酶活性的响应特征[J].江西师范大学学报(自然科学版),2018,(02):171-178.[doi:10.16357/j.cnki.issn1000-5862.2018.02.10]
　JIAN Minfei,WANG Tongtong,YU Houping,et al.The Responses of Morphology and Antioxidant Enzyme Activity of the Riparian Dominant Plant Rumex japonicus Houtt Under the Stress of Two Different Defoliation Patterns[J].Journal of Jiangxi Normal University:Natural Science Edition,2018,(02):171-178.[doi:10.16357/j.cnki.issn1000-5862.2018.02.10]
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河岸带植物羊蹄在2种去叶胁迫下的生长及酶活性的响应特征()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年02期
页码:
171-178
栏目:
化学与生命科学
出版日期:
2018-04-20

文章信息/Info

Title:
The Responses of Morphology and Antioxidant Enzyme Activity of the Riparian Dominant Plant Rumex japonicus Houtt Under the Stress of Two Different Defoliation Patterns
文章编号:
1000-5862(2018)02-0171-08
作者:
简敏菲1; 2; 王童童1; 余厚平2; 张乖乖1
1.江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西 南昌 330022; 2.江西师范大学鄱阳湖湿地与流域研究教育部重点实验室,江西 南昌 330022
Author(s):
JIAN Minfei1; 2; WANG Tongtong1; YU Houping2; ZHANG Guaiguai1
1.College of Life Science,Jiangxi Provincial Key Lab of Protection and Utilization of Subtropical Plant Resources,Jiangxi Normal University,Nanchang Jiangxi 330022,China; 2.Key Laboratory of Poyang Lake Wetland and Watershed Research,Ministry of Education
关键词:
羊蹄;  去叶模式;  去叶;  株高;  保护酶
Keywords:
Rumex japonicus;  defoliation pattern;  leaf removal;  plant height;  protective enzyme
分类号:
Q 945.78; S 511
DOI:
10.16357/j.cnki.issn1000-5862.2018.02.10
文献标志码:
A
摘要:
为探究乡土湿地植物羊蹄在采食胁迫下的形态学和生理变化规律及其响应特征,并以此为修复湿地植被生态系统的可行性提供依据,以鄱阳湖-乐安河河岸带优势物种羊蹄(Rumex japonicus)为材料,采用盆栽实验,测定在周期性连续去叶条件下,A、N 2种不同去叶模式、3种去叶强度下羊蹄的形态学以及酶活性指标变化及对比关系.结果表明:在按照叶面积进行不同强度去叶处理的A去叶模式下,羊蹄的叶片数目、植株高度比对照组和按照叶片数目进行不同强度去叶处理的N去叶模式组均有显著增加,但增加幅度随强度和时间增加而减弱; 羊蹄叶片中SOD酶活性呈现逐渐升高的趋势,在25%与50%去叶强度下,N去叶模式均大于A去叶模式; 在75%去叶强度下,结果却相反.在所有强度的A去叶模式下,羊蹄POD酶以及CAT酶活性均大于N去叶模式,但两者的变化趋势不同.在75%去叶强度下羊蹄CAT酶活性均低于其他去叶强度,CAT酶活性呈现抛物线式变化趋势.羊蹄对由人工剪除部分叶面积造成的模拟采食胁迫具有更强的适应性; 羊蹄对采食胁迫的抗逆性随去叶强度的增强而减弱,但是在75%去叶强度下羊蹄仍能进行代谢生长,这表明羊蹄对模拟采食胁迫影响具有较好的耐受性.
Abstract:
The aim of the the study was to explore the morphological and physiological change rules and characteristics response of the Rumex japonicus under the stress of defoliation and provide basis for the remediation of wetland vegetation ecosystem.The dominant plant Rumex japonicus were collected from the riparian zone of the Poyang Lake and Le'an River as the materials,soil pot experiment was conducted to explore the variation and comparison of morphology and enzyme activity index of Rumex japonicus under periodic continuous defoliation of two different defoliation patterns of 3 defoliation intensity.The results show that the number of leaves and plant height of Rumex japonicus for different intensity of defoliation of a defoliation pattern defoliated by the leaf area has an great increasement by contrast with the CK and defoliation pattern defoliated by the number of leaf defoliation,but increasement decreased with change of intensity and time.The activity of the SOD enzyme activity increased gradually over time,and the activity of the SOD enzyme activity under the N defoliation pattern was greater than that of A defoliation pattern under 25% and 50% defoliation intensity,however the SOD enzyme activity of 75% defoliation intensity was contrary.The POD and CAT enzyme activity of all defoliation intensity of pattern A is greater than the pattern N,but the change trend is different.CAT activity of two defoliation patterns of the 75% defoliation intensity were lower than other defoliation intensity,CAT enzyme activity showed a parabolic trend.In contrast,the pattern A has a stronger resistance over pattern N,while the 75% defoliation intensity had negative influence on Rumex japonicus.

参考文献/References:

[1] 胡彬,翟文静,赵警卫.河岸带植被对河流生态功能影响研究进展[J].福建林业科技,2015,42(3):233-237.
[2] 简敏菲,韩乃鹏,余厚平,等.不同水淹条件下河岸带优势植物类群的生理与生态响应特征[J].环境科学研究,2017,30(4):559-569.
[3] 简敏菲,史雅甜,陈涛,等.重金属镉、铅胁迫下湿地植物丁香蓼的生理生化特征研究[J].江西师范大学学报:自然科学版,2017,41(1):93-98.
[4] 尚小龙,余厚平,陈朴青,等.乐安河-鄱阳湖段水环境因子对重金属Cu、Pb、Cd迁移转化的影响[J].江西师范大学学报:自然科学版,2014,38(6):650-655.
[5] Elzaawely A A,Xuan T D,Tawata S.Antioxidant and antibacterial activities of Rumex japonicus Houtt Aerial parts[J].Biological and Pharmaceutical Bulletin,2005,28(12):2225-2230.
[6] Huh J Y,Cho H J,Park K J,et al.Anti-inflammatory effect of Rumex japonicus Houtt in RAW 264.7 cells[J].Koreoa Journal of Herbology,2012,27(4):99-107.
[7] 李跃强, 盛承发.叶型超补偿下棉花叶中保护酶活性的变化[J].昆虫学报,2004,47(6):780-786.
[8] 李跃强, 宣维健, 盛承发.大强度去叶对棉花叶绿素和保护酶系的影响[J].生态学报,2006,26(3):830-836.
[9] Moulinier J,Lorenzetti F,Bergeron Y.Growth and mortality of trembling aspen(Populus tremuloides)inresponse to artificial defoliation[J].Acta Oecologica,2014,55(6):104-112.
[10] Willocquet L,Aubertot J N,Lebard S,et al.Simulating multiple pest damage in varying winter wheat production situations[J].Field Crops Research,2008,107(1):12-28.
[11] Schooler S,Baron Z,Julien M.Effect of simulated and actual herbivory on alligator weed,Alternanthera philoxeroides,growth and reproduction[J].Biological Control,2006,36(1):74-79.
[12] 王春萍,雷开荣,李正国,等.低温胁迫对水稻幼苗不同叶龄叶片叶绿素荧光特性的影响[J].植物资源与环境学报,2012,21(3):38-43.
[13] 刘家尧.植物生理学实验教程[M].北京:高等教育出版社,2010:77-78.
[14] 王伟玲,王展,王晶英.植物过氧化物酶活性测定方法优化[J].实验室研究与探索,2010,29(4):26-28.
[15] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2007:164-165.
[16] 杨贤均,刘可慧,刘华,等.锰污染对酸模叶蓼生长、锰吸收及抗氧化物活性的影响[J].植物科学学报,2016,34(6):926-933.
[17] 刘铁宁,徐彩龙,谷利敏,等.高密度种植条件下去叶对不同株型夏玉米群体及单叶光合性能的调控[J].作物学报,2014,40(1):143-153.
[18] Günthardt-Goerg M S,Vollenweider P.Linking stress with macroscopic and microscopic leaf response in trees:New diagnostic perspectives[J].Environmental Pollution,2007,147(3):467-488.
[19] 孙慧珍,李辉,于伯康,等.人为去叶对紫椴幼树叶片净光合速率的影响[J].东北林业大学学报,2009,37(11):33-35.
[20] 刘辉,滕爱娣,王琳,等.去叶对不同时期菊芋生物量、株高和生长速率的影响[J].草地学报,2016,24(4):915-918.
[21] 赵威,王征宏.植物的补偿性生长[J].生物学通报,2008,43(3):12-13.
[22] 赵威,李亚鸽,王艳杰.植物补偿性光合作用的发生模式及生理机制分析[J].植物生理学报,2016,52(12):1811-1818.
[23] 李跃强,宣维健,王红托,等.棉花对棉铃虫为害超补偿作用的生理机制[J].昆虫学报,2003,46(3):267-271.
[24] Spencer D F.Response of Arundo donax L(giant reed)to leaf damage and partial defoliation[J].Journal of Freshwater Ecology,2012,27(1):77-87.
[25] 宋富强,赵俊斌,张一平,等.西双版纳区域气候变化对植物生长趋势的影响[J].植物分类与资源学报,2010,32(6):547-553.
[26] Santos M E R,Fonseca D M D,Silva G P,et al.Defoliation patterns in signal grass tillers with varying heights in the same pasture[J].Acta Scientiarum Animal Sciences,2013,35(3):259-265.
[27] Hartnett D C,Ott J P,Sebes K,et al.Coping with herbivory at the juvenile stage:Responses to defoliation and stem browsing in the African savanna tree Colophospermum mopane[J].Journal of Tropical Ecology,2012,28(2):161-169.
[28] Andréia C,Gisele P,Barcellos R S,et al.Plant responses to stresses:Role of ascorbate peroxidase in the antioxidant protection[J].Genetics and Molecular Biology,2012,35(4):1011-1019.
[29] Holley A K,Miao L,Clair D K S,et al.Redox-modulated phenomena and radiation therapy:The central role of superoxide dismutases[J].Antioxidants and Redox Signaling,2014,20(10):1567-1589.
[30] 刘国锋, 韩士群, 刘学芝,等.藻华聚集的环境效应:对漂浮植物水葫芦(Eichharnia crassipes)抗氧化酶活性的影响[J].湖泊科学,2016,28(1):31-39.
[31] Carballo I G,Llerena J L,Sánchez M E V,et al.Effects of defoliation and water restriction on total phenols and antioxidant activities in grapes during ripening[J].Journal International Des Sciences De La Vigne Et Du Vin, 2014,48(1):31-42.
[32] 李欣,江君,徐君,等.经低温锻炼后荷花不同品种对低温胁迫的生理响应差异分析[J].植物资源与环境学报,2015,24(4):76-82.
[33] Almagro L,Gómez Ros L V,Belchi-Navarro S,et al.Class III peroxidases in plant defence reactions[J].Journal of Experimental Botany,2009,60(2):377-390.
[34] Kobayashi A,Koguchi Y,Kanzaki H,et al.A new type of antimicrobial phenolics produced by plant peroxidase and its possible role in the chemical defense systems against plant pathogens[J].Zeitschrift Für Naturforschung C Journal of Biosciences,1994,49(7/8):411.
[35] Andersone U,Samsone I,Ievinsh G.Neodiprion sertifer,defoliation causes long-term systemic changes of oxidative enzyme activities in Scots pine needles[J].Arthropod-Plant Interactions,2009,3(4):209-214.
[36] Mhamdi A,Queval G,Chaouch S,et al.Catalase function in plants:a focus on Arabidopsis mutants as stress-mimic models[J].Journal of Experimental Botany,2010,61(15):4197-4220.
[37] Mhamdi A,Noctor G,Baker A.Plant catalases:peroxisomal redox guardians[J].Archives of Biochemistry and Biophysics,2012,525(2):181.
[38] 高丽丽,李淦,康正华,等.脱叶剂对棉花抗氧化酶及内源激素的影响[J].农药学学报,2016,18(4):439-446.
[39] 陈丽娜,唐明灯,艾绍英,等.Pb胁迫条件下3种叶菜的生长和生理响应及其抗性差异[J].植物资源与环境学报,2010,19(4):78-83.
[40] Hassinen V H,Tervahauta A I,Schat H,et al.Plant metallothioneins:Metal chelators with ROS scavenging activity?[J].Plant Biology,2011,13(2):225-232.

备注/Memo

备注/Memo:
收稿日期:2017-08-06
基金项目:国家自然科学基金(41461042)和江西省重大生态安全问题监控协同创新中心(JXS-EW-00)资助项目.
作者简介:简敏菲(1969-),女,江西高安人,教授,博士,主要从事鄱阳湖湿地生态与环境污染生态修复研究. E-mail: jianminfei0914@163.com
更新日期/Last Update: 2018-04-20