[1]阙艳鹏,盛占武,申佳琦,等.诺丽果粉水溶液对MCF-7人乳腺癌细胞及其内源性VEGF影响[J].江西师范大学学报(自然科学版),2018,(01):100-105.[doi:10.16357/j.cnki.issn1000-5862.2018.01.17]
　QUE Yanpeng,SHENG Zhanwu,SHEN Jiaqi,et al.The Effects of Noni's Freeze-Dried Powder on Breast Cancer MCf-7 Cell Line and Endogenous VEGF by Tumor Cells[J].Journal of Jiangxi Normal University:Natural Science Edition,2018,(01):100-105.[doi:10.16357/j.cnki.issn1000-5862.2018.01.17]
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诺丽果粉水溶液对MCF-7人乳腺癌细胞及其内源性VEGF影响()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年01期
页码:
100-105
栏目:
化学与生命科学
出版日期:
2018-02-20

文章信息/Info

Title:
The Effects of Noni's Freeze-Dried Powder on Breast Cancer MCf-7 Cell Line and Endogenous VEGF by Tumor Cells
文章编号:
1000-5862(2018)01-0100-06
作者:
阙艳鹏1; 盛占武2; 申佳琦1; 赵 帅1; 夏栋楠1; 符文英1*
1.海南大学热带农林学院生物系,海南 海口 570228; 2.中国热带农业科学院海口实验站,海南 海口 70102
Author(s):
QUE Yanpeng1; SHENG Zhanwu2;  SHEN Jiaqi1; ZHAO Shuai1; XIA Dongnan1; FU Wenying1*
1.Institute of Life Science,College of Tropical Agriculture and Forestry,Hainan University,Haikou Hainan 570228,China; 2.Haikou Experimental Station Chinese Academy of Tropical Agricultural Science,Haikou Hainan 570102,China
关键词:
诺丽;  乳腺癌细胞;  VEGF
Keywords:
noni;  Mcf-7;  VEGF
分类号:
R 737.9
DOI:
10.16357/j.cnki.issn1000-5862.2018.01.17
文献标志码:
A
摘要:
用诺丽冻干粉加入至不完全培养基中,培养人乳腺癌Mcf -7细胞,通过Cck-8、流式细胞术、TUNEL-DAPI染色等方法体外实验,比较不同处理方式下细胞的多方面生物学特性.Cck-8检测法的结果显示,经诺丽果粉水溶液处理细胞的Cck-8 吸光值低于用不完全培养基培养细胞的吸光值,并且与作用时间呈负相关性.TUNEL-DAPI双染结果显示,经诺丽果粉水溶液处理的细胞凋亡率高于正常培养的细胞,并且凋亡率与浓度呈正相关性.流式细胞术检测结果显示,经过诺丽果粉水溶液处理的细胞在1 mg·mL-1浓度下,24 h即会发生早期凋亡.酶联免疫吸附(Elisa法)检测内源性VEGF含量结果显示,经诺丽果粉水溶液处理后的细胞上清液中VEGF的含量:20,10 mg·mL-1浓度下VEGF含量降低,5,2,1 mg·mL-1浓度下VEGF含量升高.诺丽果粉水溶液对Mcf-7人乳腺癌细胞系有生长抑制作用,作用方式可能为阻碍VEGF与细胞结合.
Abstract:
Adding noni's freeze-dry powder into Incomplete medium to culture Mcf-7 cell.This paper will compare cellular biological characteristics,including Cck-8,flow cytometry,TUNEL-DAPI staining and so on.Cck-8 assay showed that the Cck-8 absorption value of the cells treated with aqueous solution of Noni fruit powder is lower than those with incomplete cultured cell light absorption value,and it has negative correlation with time.TUNEL-DAPI double staining showed that the apoptosis rate of the normal cell apoptosis is higher than the one with Noni powder aqueous solution treatment,and the apoptosis rate was positively correlated with the concentration.Flow cytometry results showed that the cells treated with Noni powder aqueous solution in the concentration of 1 mg·mL-1,would occurs early apoptosis within 24 h.Enzyme linked immunosorbent assay(Elisa method)to detect endogenous VEGF content showed that the content of VEGF reduced after treated in aqueous solution with Noni powder of 20 mg·mL-1 and 10 mg·mL-1,but VEGF content increased after treated in aqueous solution with Noni powder of 5 mg·mL-1,2 mg·mL-1 and 1 mg·mL-1 concentration.Noni powder solution inhibited the growth of Mcf-7 human breast cancer cell,and the mechanism might be by combining suppressor cell with VEGF.

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备注/Memo

备注/Memo:
收稿日期:2017-08-06
基金项目:国家自然科学基金(31360066)和海南省研究生创新课题(Hys2016-34)资助项目.
通信作者:符文英(1962-),女,海南万宁人,教授, 主要从事植物药理学研究.E-mail:fwynoni@163.com
更新日期/Last Update: 2018-02-20