[1]何昕颖,申 铁*.铁限制诱导对聚球藻转录组的影响[J].江西师范大学学报(自然科学版),2021,(02):180-187.[doi:10.16357/j.cnki.issn1000-5862.2021.02.11]
　HE Xinying,SHEN Tie*.The Effect of Iron Restriction Induction on the Transcriptome of Synechococcus[J].Journal of Jiangxi Normal University:Natural Science Edition,2021,(02):180-187.[doi:10.16357/j.cnki.issn1000-5862.2021.02.11]
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铁限制诱导对聚球藻转录组的影响()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年02期
页码:
180-187
栏目:
生命与环境科学
出版日期:
2021-04-10

文章信息/Info

Title:
The Effect of Iron Restriction Induction on the Transcriptome of Synechococcus
文章编号:
1000-5862(2021)02-0180-08
作者:
何昕颖; 申 铁*
贵州师范大学贵州省信息与计算科学重点实验室,贵州 贵阳 550001
Author(s):
HE Xinying; SHEN Tie*
Key Laboratory of Information and Computing Science of Guizhou Province,Guizhou Normal University,Guiyang Guizhou 550001,China
关键词:
聚球藻Synechococcus sp. PCC 7002;  Fe元素限制;  转录组;  高通量测序技术
Keywords:
Synechococcus sp. PCC 7002;  Fe restriction;  transcriptome;  high-throughput sequencing technology
分类号:
Q 5
DOI:
10.16357/j.cnki.issn1000-5862.2021.02.11
文献标志码:
A
摘要:
为探究Fe元素限制对聚球藻转录组的影响,该文以聚球藻Synechococcus sp. PCC 7002为研究对象,采用高通量测序技术对经过铁限制处理的聚球藻Synechococcus sp. PCC 7002进行转录组分析.以铁的3种摩尔浓度进行处理,其中每组数据重复3次实验,一共获得9组实验数据; 对照组摩尔浓度为10.900 nmol·L-1,铁限制组摩尔浓度分别为0.410、0.003 nmol·L-1.实验发现:在铁限制环境下,聚球藻通过增加对胞外铁摄取、上调光合作用相关蛋白的表达和调整代谢通路来应对缺铁环境,且铁限制程度与差异基因数量成正相关关系.此外,在强缺铁时通过下调CRISPR系统中某些核酸酶的表达来影响聚球藻抵御外源病毒感染的能力,其转运磷酸基团ABC转运蛋白表达水平下降,从而极大地影响了聚球藻的生存.
Abstract:
In order to explore the effect of Fe restriction on the Synechococcus transcriptome,Synechococcus sp. PCC 7002 is as the research object in the study.High-throughput sequencing is used to analyze the transcriptome of Synechococcus sp. PCC 7002 treated with iron restriction.Three different concentrations of iron are used,and the experiment is repeated three times for each set of data,a total of nine sets of experimental data.The concentration of the control group is 10.900 nmol·L-1,and the iron-restricted group is 0.410 nmol·L-1 and 0.003 nmol·L-1,respectively.It is found that in response to iron-deficiency environments,Synechococcus increases extracellular iron uptake,upregulates the expression of photosynthesis-related proteins and adjusts metabolic pathways,and the degree of iron-restriction is positively correlated with the number of differential genes.In addition,strong iron deficiency affects the ability of Synechococcus sp. PCC 7002 to resist foreign virus infection by down-regulating the expression of certain nucleases in the CRISPR system,and the expression level of the ABC transporter that transported phosphate groups also decreased,which greatly affects its survival.

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备注/Memo

备注/Memo:
收稿日期:2020-11-25
基金项目:国家自然科学基金(31760254)资助项目.
通信作者:申 铁(1982—),男,贵州遵义人,教授,博士,主要从事转录组学、代谢流量组学、基因组学等研究.E-mail:20421980@qq.com
更新日期/Last Update: 2021-04-10