[1]甘湘庆,庞向东,秦宗会.溴甲酚绿光度法测定盐酸吡格列酮的含量[J].江西师范大学学报(自然科学版),2012,(02):196-199.
 GAN Xiang-qing,PANG Xiang-dong,QIN Zong-hui.Spectrophotometric Method for Determination of Pioglitazone Hydrochloride with Bromocresol Green[J].,2012,(02):196-199.
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溴甲酚绿光度法测定盐酸吡格列酮的含量()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2012年02期
页码:
196-199
栏目:
出版日期:
2012-03-01

文章信息/Info

Title:
Spectrophotometric Method for Determination of Pioglitazone Hydrochloride with Bromocresol Green
作者:
甘湘庆;庞向东;秦宗会
长江师范学院化学化工学院,重庆,408100
Author(s):
GAN Xiang-qing;PANG Xiang-dong;QIN Zong-hui
关键词:
分光光度法盐酸吡格列酮溴甲酚绿
Keywords:
spectrophotometric method pioglitazone hydrochloride bromocresol green
分类号:
O657.3
文献标志码:
A
摘要:
在pH值为8.0的Britton-Robinson缓冲介质中,盐酸吡格列酮与溴甲酚绿反应,形成离子缔合物,最大褪色波长位于616 nm处,同时在450 nm处出现增色效应,在最大褪色与最大增色波长处,盐酸吡格列酮的浓度分别在2.81×10-6~1.52×10-5 mol/L和7.70×1 0-6 1.54×1 0-5 mol/L范围内遵守比尔定律,表观摩尔吸光系数ε分别为6.08× 104和1.56×104 L/(mol·cm),检测限分别为1.99×10-7和2.51×10-7 mol/L.若用双波长叠加,检测限更低.方法用于市售药品中盐酸吡格列酮含量的测定,所得结果满意.以血浆和尿液样品为基体加入标准溶液作回收率试验,测定回收率在96.7;~103.6;之间,测定值的相对标准偏差均小于3.70;.
Abstract:
In Britton-Robinson buffer medium of pH 8.0, bromocresol green reacts with pioglitazone hydrolchloride, forming ion-association complexes, maximum fading wavelength of complexes is 616 nm, the maximum absorb wavelength locates 450 nm, the concentration of pioglitazone hydrolchloride follows Beer’s law for the tight absorption in the different range of 2.81×10?6 ~ 1.52×10?5 mol/L and 7.70×10?6~1.54×10?5 mol/L respectively, the apparent molar absorptivities are 6.08×104 L/(mol?cm) and 1.56×104 L/(mol?cm) and the detection limits are 1.99×10?7 mol/L and 2.51×10?7 mol/L respectively. If the determination process is dealt with double wavelength, Lower the detection limits. Samples of tablets and capsules of pioglitazone hydrochloride were analyzed by the proposed method, and the amounts of PGH found were checked quite well with the labelled values, the results are satisfactory. Recovery and precision of the method were tested by the standard addition method using samples of human blood serum and urine as matrixes, values of recovery found were in the range of 96.7% and 103.6%, and values of RSD found were less than 3.70%.

参考文献/References:

[1] Smith U. Pioglitazone: mechanism of action [J]. International Journal of Clinical Practice, 2001, 121: 13-18.
[2] Lawrence J M, Reckless J P. Actos (pioglitazone): a new treatment for type 2 diabetes [J]. Hospital Medicine, 2001, 62(7): 411-416.
[3] 秦宗会, 沈光明, 刘於生, 等. 氯酚红分光光度法测定盐酸吡格列酮 [J]. 分析试验室, 2007, 27(10): 15-18.
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[7] Pattana Sripalakit, Penporn Neamhom, Aurasorn Saraphanchotiwitthaya. High- performance liquid chromatographic method for the determinationof pioglitazone in human plasma using ultraviolet detection and its application to a pharmacokinetic study [J]. Journal of Chroma- tography B, 2006, 843: 164-169.
[8] 戴俊东, 金笃嘉, 刘玉玲. RP-HPLC法测定盐酸吡格列酮含量及有关物质的方法学研究 [J]. 药物分析杂志, 2001, 21(1): 36-39.
[9] Maeshiba Y, Kiyota Y, Yamashita K, et al. Disposition of the new antidiabetic agent pioglitazone in rats, dogs, and monkeys [J]. Arzneimittel- Forschung, 1997, 47(1): 29-35.
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备注/Memo

备注/Memo:
教育部春晖计划(Z2009-1-63001);重庆市教育委员会科学技术(KJ111320)
更新日期/Last Update: 1900-01-01