[1]孙启中,陶欣艺,周勖,等.抗过敏中草药活性成分的药效评价[J].江西师范大学学报(自然科学版),2012,(06):554-557.
 SUN Qi-zhong,TAO Xing-yi,ZHOU Xu,et al.The Anti-Allergy Effects for Active Constituents Extracted from Chinese Medicinal Herbs[J].,2012,(06):554-557.
点击复制

抗过敏中草药活性成分的药效评价()
分享到:

《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2012年06期
页码:
554-557
栏目:
出版日期:
2012-12-01

文章信息/Info

Title:
The Anti-Allergy Effects for Active Constituents Extracted from Chinese Medicinal Herbs
作者:
孙启中;陶欣艺;周勖;任宇红
华东理工大学生物反应器工程国家重点实验室, 上海 200237
Author(s):
SUN Qi-zhong TAO Xing-yi ZHOU Xu REN Yu-hong
关键词:
中草药肥大细胞脱颗粒组胺抑制
Keywords:
acnesChinese herbmast celldegranulationhistaminemechanism
分类号:
Q58
文献标志码:
A
摘要:
肥大细胞受到抗原刺激后释放组胺是过敏反应的重要过程,对组胺释放的抑制能力是评价抗过敏药物的重要因素.选取多种传统中医记载具有抗过敏作用的中草药,检测了其在体外对肥大细胞脱颗粒并释放组胺的抑制效果.在具有明显效果的中草药中,土茯苓与芍药对于抑制肥大细胞脱颗粒最为突出, IC50分别为62.5μg/mL和113.8μg/mL.土茯苓的突出药效(药物浓度为500μg/mL时抑制率达到95.63;)未曾被报道,具有研究与开发价值.对其机理的初步研究表明蛋白激酶A可能是土茯苓抑制肥大细胞脱颗粒的重要靶点.
Abstract:
Mast cell is considered to be a key actor for the allergic inflammation. Upon presentation of the antigen they could release histamine. The inhibition of histamine releasing is usually used as major element to evaluate the anti-allergy activity of drugs. Diverse herbs which released allergy and inflammation' symptom, were selected their depressant effect on mast cells' degranulation and the release amount of histamine in vitro were detected. Among the efficient herbs, Glabrous Greenbrier Rhizome and Paeonia Veitchii showed more rejection capability on mast cells' degranulation as the IC50 of these two kinds of herb were 62.5 μg/mL and 113.8 μg/mL, respectively. Gla-brous Greenbrier Rhizome is worth to be studied due to its conspicuous efficient (the inhibition ratio is 95.63;with the concentration of 500μg/mL) which has not been reported. The mechanism research suggested that protein kinase A may be one of the target point of Glabrous Greenbrier Rhizome's depressant effect on mast cells' de-granulation.

参考文献/References:

[1] Kurosawa M, Parker C W.A phosphaiidylinositol kinase in rat mast cell granules [J]. J Immunol, 1986, 136:616-622.
[2] Benyon R C, Church M K, Holgate S T.IgE-dependent activation of mast cells is not associated with enhanced phospholipid methylation [J]. J Immunol, 1986, 35:2535-2544.
[3] Yasmin Churcher, David Allan D.Gomperts.Relationship between arachidonate generation and exocytosis in permeabilized mast cells [J]. Biochem J, 1990, 266:157-163.
[4] Kennerly D A, Sullivan T J, Parker C W.Activation of phospholipid metabolism during mediator release from stimulated mast cells [J]. J Immunol, 1979, 122:152-159.
[5] 蒋红玲, 向军俭. 食物过敏中肥大细胞释放组胺的研究进展 [J]. 医学理论与实践, 2007, 20: 269-271.
[6] 莫雅贞, 何韶衡. 天然成分对肥大细胞的影响 [J]. 实用医学杂志, 2006, 22:2070-2072.
[7] 张承烈. 抗过敏中药研究进展 [J]. 浙江中西医结合杂志, 2005, 15(4): 253-255.
[8] 向军俭, 陈华粹. 组胺的荧光测定法的研究 [J]. 中国医学科学院学报, 1981, 3:183-186.
[9] Evans A T, Sharma P, Ryves W J, et al. TPA and resiniferatoxin-mediated activation of NADPH-oxidase: A possible role for Rx-kinase augmentation of PKC [J]. FEBS Letters, 1990, 267(2): 253-256.
[10] Michael Gschwendt, Hanno Leibersperger, Walter Kittstein, et al.Protein kinase Cζ and n in murine epidermis TPA induces down-regulation of PKCn but not PKCζ [J]. FEBS Letters, 1992, 307(2):151-155.
[11] Claudia Martini, Maria Plaza, Maria del C Vila. PKA-d ependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation [J]. Molecular and Cellular Endocrinology, 2009, 298(1/2): 42-47.
[12] Kevin A W Lee.Transcriptional regulation by cAMP [J]. Current Opinion in Cell Biology, 1991, 3: 953-959.

更新日期/Last Update: 1900-01-01