[1]孙婉菊,王筱兰.废弃菌体水解液作氮源发酵产腈水合酶的研究[J].江西师范大学学报(自然科学版),2013,(05):530-534.
 SUN Wan-ju,WANG Xiao-lan.The Research on the Fermentation Production of Nitrile Hydratase by Using Waste Cell Solution as Nitrogen Source[J].,2013,(05):530-534.
点击复制

废弃菌体水解液作氮源发酵产腈水合酶的研究()
分享到:

《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2013年05期
页码:
530-534
栏目:
出版日期:
2013-10-31

文章信息/Info

Title:
The Research on the Fermentation Production of Nitrile Hydratase by Using Waste Cell Solution as Nitrogen Source
作者:
孙婉菊;王筱兰
江西师范大学生命科学学院,江西南昌,330022
Author(s):
SUN Wan-ju;WANG Xiao-lan
关键词:
废弃菌体水解液氮源腈水合酶
Keywords:
waste cellsolutionnitrogen sourcenitrile hydratase
分类号:
TQ920
文献标志码:
A
摘要:
工业生产中微生物细胞在发酵结束后常被作为废弃物丢弃,容易造成环境污染和资源浪费.对废弃细胞进行破壁处理,废弃菌体水解液可替代酵母膏作有机氮源用于发酵产腈水合酶.通过采用超声波、冻融、热碱3种方法破碎细胞,测定水解液中氨基氮含量的高低,确定选择热碱法为最佳细胞破碎方法.通过正交实验选出最优破壁条件为:N aOH加入量5g.L-1,水解温度100℃,水解时间1.5h.在最佳破壁条件下,细胞破碎液中氨基氮含量为24.5 g· L-1.并考察了酵母膏与水解液的不同配比对腈水合酶发酵酶活的影响,确定了酵母膏与废弃菌体水解液的最佳配比为3:2.实验结果表明:利用热碱法处理废弃菌体的水解液作为氮源部分代替酵母膏,发酵酶活为1 063万U·mL-1,而完全利用酵母膏作为氮源的发酵酶活为1 055万U·mL-1.
Abstract:
The waste cells in fermentative production were often discarded directly,causing environmental pollution and the waste of resources.Nitrogen source in the waste cell solution can be reused in the production of nitrile hydratase instead of yeast extract when the cell wall was destroyed.Ultrasonic,freezing and thawing,and hot alkaline were investigated for cell wall-breaking.The results showed that hot alkaline was more effective for higher amino nitrogen content.The optimum reaction conditions were NaOH 5 g·L,100 ℃,1.5 h,which is elected by orthogonal experiment,then the amino nitrogen concentration reached 24.5 g· L.The suitable ratio(3:2)was determined through its impact on the nitrile hydratase activity by using waste cell solution replaced a certain amount of yeast extract as nitrogen source.Compared with the original medium's enzyme activity 1.055×10 U·mL whose nitrogen source was complete yeast extract,the nitrile hydratase activity was 1.063×10 U·mL in the medium containing waste cell solution.

参考文献/References:

[1] 白雪飞.发酵产丁二酸过程中废弃细胞的循环利用 [J].生物工程学报,2010,26(9):1276-1280.
[2] Tatjana V M,Marica R,Slavica S M.Utilization of baker's yeast(Saccharomyces cerevisiae)for the production of yeast extract:effects of different enzymatic treatments on solid,protein and carbohydrate recovery [J].J Serb Chem Soc,2007,72(5):451-457.
[3] 姜岷,王倩楠,陈可泉,等.啤酒废酵母酶解液作为有机氮源厌氧发酵制备丁二酸的研究 [J].食品科技,2007,32(10):238-241.
[4] Lin S K C,Du C,Koutinas A,et al.Substrate and product inhibition kinetics in succinic acid production by actinobacillus succinogenes [J].Biochem Eng J,2008,41(2):128-131.
[5] 王丽娜,李夏兰.废弃啤酒酵母细胞提取ADH的研究:其狭路相逢破碎的研究 [J].福建化工,1999(2):9-11.
[6] 宁正祥.食品成分分析手册 [M].北京:中国轻工业出版社,1998:119-124.
[7] 黄伟波.一株腈水合酶产生菌发酵过程的控制及其酶学性质的研究 [D].南昌:江西师范大学,2011.
[8] 岑沛林,蔡谨.工业微生物 [M].北京:化学工业出版社,2000.
[9] 闫宝松,马凤.废弃菌渣二次利用及环保处理方法 [J].中国林副特产,2005(4):27.
[10] 修志龙,姜炜,苏志国.细胞破碎技术的研究进展和发展方向 [J].化工进展,1994(1):15-21.

备注/Memo

备注/Memo:
江西省科技支撑计划(2009JX00564)
更新日期/Last Update: 1900-01-01