[1]杨彩玲,韩金红,王伟新,等.盐酸埃克替尼对ACC-M细胞MMP-2、E-cadherin蛋白表达的影响[J].江西师范大学学报(自然科学版),2014,(04):367-369.
　YANG Cai-ling,HAN Jin-hong,WANG Wei-xin,et al.Influence of Icotinib Hydrochloride uPon the Protein ExPression of ACC-M Cells MMP-2 and E-cadherin[J].Journal of Jiangxi Normal University:Natural Science Edition,2014,(04):367-369.
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盐酸埃克替尼对ACC-M细胞MMP-2、E-cadherin蛋白表达的影响()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2014年04期
页码:
367-369
栏目:
出版日期:
2014-08-31

文章信息/Info

Title:
Influence of Icotinib Hydrochloride uPon the Protein ExPression of ACC-M Cells MMP-2 and E-cadherin
作者:
杨彩玲;韩金红;王伟新;崔卫刚
新乡医学院第一附属医院口腔外科,河南 卫辉,453100；新乡医学院基础医学院,河南 新乡,453003
Author(s):
YANG Cai-ling；HAN Jin-hong；WANG Wei-xin；CUI Wei-gang
关键词:
盐酸埃克替尼; ACC-M 细胞; MMP-2; E-cadherin
Keywords:
icotinib hydrochloride; ACC-M cells; MMP-2; E-cadherin
分类号:
R734.2
文献标志码:
A
摘要:
盐酸埃克替尼是一种表皮生长因子受体酪氨酸激酶抑制剂，利用免疫细胞化学方法和间接免疫荧光联合流式细胞技术，检测它在体外对人涎腺腺样囊性癌细胞株（ACC-M）MMP-2、E-cadherin 蛋白表达的影响。结果表明：盐酸埃克替尼可降低 MMP-2蛋白表达，增加 E-cadherin 蛋白表达，可抑制人涎腺腺样囊性癌 ACC-M 细胞的浸润、转移。
Abstract:
Icotinib hydrochloride is a kind of epidermal growth factor receptor tyrosine kinase inhibitor,whose influ-ence upon the protein expression of ACC-M cells MMP-2 and E-cadherin can be tested by way of immunocytochem-istry and the technique of indirect immunofluorescence together with flow cytometry. The results show that icotinib hydrochloride can reduce the protein expression of MMP-2,but increase that of E-cadherin,thus it can be used to restrain the infiltration and metastasis of ACC-M cells.

参考文献/References:

[1] Gao Zhenzhen,Chen Wei,Zhang Xiaohua,et al.Icotinib,a potent and specific EGFR tyrosine kinase inhibitor,inhibits growth of squamous cell carcinoma cell line A431 through negatively regulating AKT signaling [J].Biomed Pharmacother,2013,67(5):351-356.
[2] Camidge D R.Icotinib:kick-starting the Chinese anticancer drug industry [J].Lancet Oncol,2013,14(10):913-914.
[3] Shi Yuankai,Zhang Li,Liu Xiaoging,et al.Icotinib versus gefitinib in previously treated advanced non-small-cell lung cancer(ICOGEN):a randomised,double-blind phase 3 non-inferiority trial [J].Lancet Oncol,2013,14(10):953-961.
[4] 杨彩玲,韩新光.盐酸埃克替尼对人舌鳞状细胞癌Tca8113细胞增殖的影响 [J].郑州大学学报:医学版,2008,43(4):701-703.
[5] 杨彩玲,韩新光,于春亚.盐酸埃可替尼对人舌鳞状细胞癌Tca8113细胞周期及CyclinD1、P27表达的影响 [J].郑州大学学报:医学版,2011,46(1):82-85.
[6] Taniwaki K,Fukamachi H,Komori K,et al.Stroma-derived matrix metalloproteinase(MMP)-2 promotes membrane type 1-MMP-dependent tumor growth in mice [J].Cancer Res,2007,67(9):4311-4319.
[7] 卢友光,周鸿鹰,丁林灿,等.涎腺腺样囊性癌高、低转移细胞系基因表达谱及基质金属蛋白酶表达差异 [J].中华医学遗传学杂志,2006,23(5):505-510.
[8] Eimoneim H M,Zaghloul N M.Expression of E-cadherin,N-cadherin and snail and their correlation with clinicopathological variants:an immunohistochemical study of 132 invasive ductal breast carcinomas in Egypt [J].Clinics(Sao Plaulo),2011,66(10):1765-1771.
[9] Hashimoto T,Soeno Y,Maeda G,et al.Progression of oral squamous cell carcinoma accompanied with reduced e-cadherin expression but not cadherin switch [J].Plos One,2012,7(10):e47899.
[10] Wells A,Yates C,Shepard C R,et al.E-cadherin as an indicator of mesenchymal to epithelial reverting transitions during the metastatic seeding of disseminated carcinomas [J].Clin Exp Metastasis,2008,25(6):621-628.
[11] Yauch R L,Januaro T,Eberhard D A,et al.Epithelial versus mesenchymal phenotype determines in vitro sensitivity and predicts clinical activity of erlotinib in lung cancer patients [J].Clin Cancer Res,2005,11(24):8686-8698.
[12] Witta S E,Gemmill R M,Hirsch F R,et al.Restoring E-cadherin expression increases sensitivity to epidermal growth factor receptor inhibitors in lung cancer cell lines [J].Cancer Res,2006,66(2):944-950.

备注/Memo

备注/Memo:
新乡医学院高学历人才基金(GXLZZ2008-09)
更新日期/Last Update: 1900-01-01