[1]赖政,李希茜,汪涯,等.蛇足石杉中石杉碱甲含量测定及其时空动态[J].江西师范大学学报(自然科学版),2014,(05):489-495.
 LAI Zheng,LI Xi-xi,WANG Ya,et al.The Determination of Huperzine A and Temporal and Spatial Variation of Huperzia serrata[J].,2014,(05):489-495.
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蛇足石杉中石杉碱甲含量测定及其时空动态()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2014年05期
页码:
489-495
栏目:
出版日期:
2014-10-31

文章信息/Info

Title:
The Determination of Huperzine A and Temporal and Spatial Variation of Huperzia serrata
作者:
赖政;李希茜;汪涯;颜日明;张志斌;朱笃
江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西 南昌 330022;江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西 南昌 330022; 江西中医药大学科技学院,江西 南昌 330025;江西科技师范大学生命科学学院,江西省生物加工过程重点实验室,江西 南昌 330013;江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西 南昌 330022; 江西科技师范大学生命科学学院,江西省生物加工过程重点实验室,江西 南昌 330013
Author(s):
LAI Zheng;LI Xi-xi;WANG Ya;YAN Ri-ming;ZHANG Zhi-bin;ZHU Du
关键词:
蛇足石杉石杉碱甲含量测定时空动态
Keywords:
Huperzia serratahuperzine Acontent detectiontemporal and spatial variation
分类号:
R282
文献标志码:
A
摘要:
采用 HPLC 法首次测定江西庐山和井冈山蛇足石杉不同时期、不同组织部位中石杉碱甲(HupA)的含量.结果表明,蛇足石杉中 HupA 含量随着季节的推移逐渐增加,庐山和井冈山蛇足石杉植株 HupA含量均在每年12月达到最高,分别为211.9μg·g -1和325.9μg·g -1.蛇足石杉中不同组织部位 HupA含量各不相同,茎部和叶部 HupA 含量较高,且表现出相同的变化趋势,根部含量较少.本研究揭示了蛇足石杉中 HupA 含量的时空动态变化规律,从而为合理利用该稀缺植物资源提供科学参考.
Abstract:
This was the first time to use HPLC to detect the contents of huperzine A(HupA)in the whole plant and different organs of Huperzia serrata from Mount Lushan and Mount Jinggang in Jiangxi Province at different seasons. The results showed that the whole plant content of HupA collected from Mount Lushan and Mount Jinggang in-creased with season changed and reached the maximum in December which reached to 211. 9 μg · g - 1 and 325. 9 μg·g - 1 . The content of HupA in different organs collected from two sample sites were various. Stem and leaf contained more HupA,while only a little HupA was detected in root. The results showed the temporal and spa-tial dynamic change rule of HupA in H. serrata,and may provided scientific reference for the reasonable use of rare plant resources.

参考文献/References:

[1] 刘嘉森,俞超美,周有作,等.石杉碱甲和石杉碱乙的化学研究 [J].化学学报,1986(10):1035-1040.
[2] Ma Xiaoqiang,Tan Changheng,Zhu Dayuan,et al.Huperzine A from Huperzia species:An ethnopharmacolgical review [J].Journal of Ethnopharmacology,2007,113(1):15-34.
[3] 殷帅文,刘丽萍,王安萍,等.15种植物不同溶剂萃取物乙酰胆碱酯酶抑制活性的初步研究 [J].井冈山大学学报:自然科学版,2012,33(4):52-56.
[4] 吴荭,庄平,冯正波,等.中国蛇足石杉资源调查与评估 [J].自然资源学报,2005,20(1):59-67.
[5] 张君诚,邢建宏,宋育红,等.药用植物蛇足石杉研究新进展 [J].中国野生植物资源,2008,27(2):1-5.
[6] 王峻,吴伟,潘胜利.HPLC法测定6种石杉科植物中石杉碱甲的含量 [J].中草药,2003,34(7):607-608.
[7] 孙远明,余红英,杨跃生,等.HPLC法测定蛇足石杉中石杉碱甲含量 [J].中草药,2002,33(12):1078-1080.
[8] 顾月华,吴庆庆.高效液相色谱法测定蛇足石杉中石杉碱甲的含量 [J].中国药理学通报,2005,21(8):1017-1018.
[9] 郭水良,李沛玲,李似姣,等.石杉碱甲的含量测定方法及在蛇足石杉不同产地居群的含量比较 [J].浙江师范大学学报:自然科学版,2006,29(2):177-180.
[10] 黄静,杨理明,李齐激,等.HPLC法测定台江产千层塔中的石杉碱甲的含量 [J].贵州医药,2007,31(7):645.
[11] 杨理明,黄静,李齐激,等.习水产蛇足石杉石杉碱甲的含量测定 [J].贵州医药,2007,31(7):646.
[12] 杜次,彭清忠,田向荣,等.湘西蛇足石杉中石杉碱甲、乙和丙含量的测定 [J].广西植物,2013,33(3):406-409.
[13] 郑雅媗,刘海元,张方方,等.HPLC法测定蛇足石杉及闽浙马尾杉不同部位的石杉碱甲含量 [J].福建中医院大学学报,2013,23(3):42-43.
[14] Ma Xianqiang,Tan Changheng,Zhu Dayuan,et al.Is there a better source of Huperzine A than Huperzia serrata? Huperzine A content of Huperziaceae species in China [J].Journal of Agricultural and Food Chemistry,2005,53(5):1393-1398.
[15] Jason Q D Goodger,Anne L Whincup,Ashley R Field,et al.Variation in Huperzine A and B in Australasian Huperzia species [J].Biochemical Systematics and Ecology,2008,36:612-618.
[16] 黄骥,赵爱玲.蛇足石杉石杉碱甲含量的动态变化分析 [J].中国中药杂志,2010,35(19):2511-2515.
[17] 王德立,甘炳春,齐耀东,等.蛇足石杉不同生长阶段中石杉碱甲含量变化的地区差异及生境影响的比较观察 [J].中国新药杂志,2014,23(3):326-332.
[18] Zhu Du,Wang Jixiang,Zeng Qinggui,et al.A novel endophytic Huperzine A:producing fungus,Shiraia sp.Slf14,isolated from Huperzia serrata [J].Journal of Applied Microbiology,2010,109(4):1469-1478.
[19] Zhang Zhibin,Zeng Qinggui,Yan Riming,et al.Endophytic fungus Cladosporium cladosporioides LF70 from Huperzia serrata produces Huperzine A [J].World Journal of Microbiology and Biotechnology,2011,27(3):479-486.
[20] Wang Ya,Zeng Qinggui,Zhang Zhibin,et al.Isolation and characterization of endophytic huperzine A:producing fungi from Huperzia serrata [J].Journal of Industrial Microbiology & Biotechnology,2011,38(9):1267-1278.
[21] 汪涯,颜日明,曾庆桂,等.一株产石杉碱甲的蛇足石杉内生真菌的分离和鉴定 [J].菌物学报,2011,30(2):255-262.
[22] 汪涯,曾庆桂,张志斌,等.蛇足石杉内生真菌分离及其抑制乙酰胆碱酯酶活性研究 [J].中国中药杂志,2011,36(6):734-740.
[23] Yan Riming,Zhang Zhibin,Wang Ya,et al.Efficient strategy for maintaining and enhancing the huperzine A production of Shiraia sp.Slf14 through inducer elicitation [J].Journal of Industrial Microbiology & Biotechnology,2014,41:1175-1179.

备注/Memo

备注/Memo:
国家自然科学基金(81260617);江西省自然科学基金(20142BAB214020);江西省研究生创新基金(YC2013-S106)
更新日期/Last Update: 1900-01-01