[1]林春花.超高效合相色谱-质谱法测定大豆油中5种脂肪酸含量[J].江西师范大学学报(自然科学版),2015,(02):162-165.
 LIN Chunhua.The Detection of Five Fatty Acids in Soybean Oil by Ultra Performance Convergence Chromatography-Mass Spectrometry[J].,2015,(02):162-165.
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超高效合相色谱-质谱法测定大豆油中5种脂肪酸含量()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
162-165
栏目:
出版日期:
2015-04-10

文章信息/Info

Title:
The Detection of Five Fatty Acids in Soybean Oil by Ultra Performance Convergence Chromatography-Mass Spectrometry
作者:
林春花
江西师范大学国家单糖化学合成工程技术研究中心,江西 南昌 330027
Author(s):
LIN Chunhua
关键词:
超高效合相色谱-质谱法 大豆油 脂肪酸 未衍生化
Keywords:
ultra-performance convergence chromatography-mass spectrometry soybean oil fatty acids underivatization
分类号:
O 657.63
文献标志码:
A
摘要:
建立了大豆油中5种脂肪酸的超高效合相色谱-质谱(UPC-MS)分析方法,这5种脂肪酸分别为棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸.待测物经皂化反应后,用正己烷溶解,经ACQUITY UPC BEH 2-EP色谱柱分离,以CO2-甲醇/乙腈(体积比为1:1)为流动相进行梯度洗脱,通过质谱检测器测定、外标法定量.方法的最低检出限为0.07 mg·L,脂肪酸的加标回收率为90.50;~105.38;,相对标准偏差为0.81;~2.93;.结果表明,该方法灵敏度高、分离效果好、测定结果准确.
Abstract:
The 5 fatty acids in soybean oil were analyzed using ultra-performance convergence chromatography,including palmitic acid,stearic acid,oleic acid,linoleic acid and linolenic acid.The sample was dissolved by n-hexane after saponification reaction.The fatty acids were separated on the column of ACQUITY UPC BEH 2-EP by gradient elution with carbon dioxide and V(methano):V(acetonitrile)=1:1 system,and finally detected by MS detector.The limits of detection of target compounds in the method were 0.07 mg·L.The recoveries were from 90.50; to 105.38; at three spiked levels with the relative standard deviations lower than 3.0;.The method,with high sensitivity,good separation effect and high recovery,was successfully used to detect the underivatization fatty acids in soybean oil.

参考文献/References:

[1] 穆同娜,孙婷,吴燕涛,等.3种食用植物油中不饱和脂肪酸含量调查 [J].粮油食品科技,2011,3(19):36-38.
[2] 曾建立,杜泽学,陈艳凤.气相色谱分析未衍生化脂肪酸及其甲酯 [J].石油炼制与化工,2012,43(7):104-109.
[3] 蒋秀琴,刘立成,赵福忠,等.常见植物油脂肪酸含量的分析 [J].饲料博览,2010(3):27-30.
[4] 郑月明,冯峰,国伟,等.全2维气相色谱-四极杆质谱法检测植物油脂中脂肪酸 [J].色谱,2012,11(30):1166-1171.
[5] 朱笃,徐曲.青葙籽油脂肪酸组成的分析 [J].江西师范大学学报:自然科学版,2002,26(2):110-112.
[6] 回瑞华,候冬岩,李铁纯,等.棉籽油中脂肪酸不同的酯化方法与气相色谱-质谱分析 [J].质谱学报,2005,26(2):90-92.
[7] 徐桂转,梁新,苏惠,等.利用气相色谱分析生物柴油中脂肪酸甲酯含量研究 [J].安徽农业科学,2008,36(28):12090-12091.
[8] Ghiaci M,Aghabarari B,Gil A.Production of biodiesel by esterification of natural fatty acids over modified organoclay catalysts [J].Fuel,2011,90(11):3382-3389.
[9] 巫淼鑫,邬国英,韩瑛,等.6种食用植物油及其生物柴油中脂肪酸成分的比较研究 [J].中国油脂,2003,28(12):65-67.
[10] 张大华,李丽霞.液相色谱法测定生物柴油中多种脂肪酸甲酯 [J].粮食与油脂,2007(12):41-43.
[11] Wood R,Lee T.High-performance liquid chromatography of fatty acids:Quantitative analysis of saturated,monoenoic,polyenoic and geometrical isomers [J].Journal of Chromatography A,1983,254:237-246.
[12] 佘珠花.气相色谱法中油脂脂肪酸衍生化方法及其选择 [J].粮食加工,2004(6):64-66.
[13] 孟哲,文大为,廖一平,等.快速气相色谱法测定食用油中的为衍生化长链脂肪酸 [J].色谱,2007,25(2):254-257.
[14] 李一哲,包桂蓉,王华.超高效液相色谱法测定生物柴油中的11种脂肪酸及其甲酯 [J].色谱,2008,26(4):494-498.
[15] 徐永威,孙庆龙,黄静,等.Waters ACQUITY UPC仪器结构和性能特点 [J].现代仪器,2012,18(5):45-48.
[16] 邹洁,赵维佳,汪海峰,等.大豆油中掺伪棕榈油的检测方法研究 [J].中国油脂,2009,34(04):73-76.

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更新日期/Last Update: 1900-01-01