[1]熊瑶瑶,敖武,李怡,等.3株拮抗内生放线菌的鉴定及其抑菌活性初步研究[J].江西师范大学学报(自然科学版),2015,(02):171-176.
 XIONG Yaoyao,AO Wu,LI Yi,et al.The Identification of Three Antagonistic Endophytic Actinomycete and Their Antimicrobial Activities[J].Journal of Jiangxi Normal University:Natural Science Edition,2015,(02):171-176.
点击复制

3株拮抗内生放线菌的鉴定及其抑菌活性初步研究()
分享到:

《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
171-176
栏目:
出版日期:
2015-04-10

文章信息/Info

Title:
The Identification of Three Antagonistic Endophytic Actinomycete and Their Antimicrobial Activities
作者:
熊瑶瑶;敖武;李怡;何晓峰;张志斌;朱笃
1.江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西 南昌 330022; 2.江西科技师范大学生命科学学院,江西省生物加工过程重点实验室,江西 南昌 330013; 3.江西博雅生物制药股份有限公司,江西 抚州 344000
Author(s):
XIONG YaoyaoAO WuLI YiHE XiaofengZHANG ZhibinZHU Du
关键词:
内生放线菌 链霉菌属 多相分类 抑菌活性
Keywords:
endophytic actinomycetes streptomyces polyphasic taxonomyantimicrobial activity
分类号:
S 718.81
文献标志码:
A
摘要:
对分离自江西东乡野生稻根部的3株内生放线菌进行鉴定和抑菌活性研究,根据菌株的形态与培养特征、生理生化特性及16S rRNA基因序列系统发育分析,将菌株FRo1、FRo2和FRo3分别鉴定为委内瑞拉链霉菌、娄彻氏链霉菌和肉质链霉菌.采用管碟法和菌丝生长速率法分析菌株拮抗病原细菌与病原真菌的活性.结果表明:菌株FRo1发酵液对所有测试细菌均有抑制作用,显示较广谱抑细菌活性; 菌株FRo2显示出较强抑制金黄色葡萄球菌和7种病原真菌的活性,其中对金黄色葡萄球菌抑菌直径为22.33 mm,对小麦赤霉、水稻纹枯病菌、车前草核盘菌及胶胞炭疽菌的抑制率分别为70;、45;、58;和65;; 而菌株FRo3发酵液对金黄色葡萄球菌、枯草芽孢杆菌及伤寒杆菌表现出不同程度的抑制,除车前草核盘菌外对所测试的病原真菌均有抑制活性,但抑制率均低于50;,这些菌株抑菌效果良好,显示出较好的生防潜力.
Abstract:
Antagonistic strains FRo1,FRo2 and FRo3 were identified,and their antimicrobial activity were assessed,which obtained from roots of Dongxiang wild rice (Oryza rufipogon).Strains FRo1,FRo2 and FRo3 was identified as Streptomyces venezuelae,Streptomyces rochei and Streptomyces carnosus by morphological and cultural traits,physiobiochemical characteristics and 16S rRNA gene sequence analysis.The inhibition effects of FRo1,FRo2 and FRo3 against pathogenic bacteria and pathogenic fungi in vitro were evaluated by agar disc diffusion method and growth rate method.The FRo1 fermentation broth showed a wide pectrum of inhibitory activity against tested bacteria.FRo2 fermentation broth had obviously inhibitory activity against staphylococcus aureus and seven pathogenic fungi.The inhibition zone of FRo2 against staphylococcus aureus was about 22.33 mm,and the inhibition ratios of FRo2 against pathogenic fungi Fusarium graminearumt,Rhizoctonia solani,Sclerotinia scleroliorum and Colletotrichum gloeosporioides were 70;、45;、58; and 65;,respectively.While FRo3 had different inhibition effects on Staphylococcus aureus,Bacillus subtilis and Typhoid bacillus,and the inhibition activity against tested pathogenic fungi except Sclerotinia scleroliorum was no more than 50;.The strains had better antibacterial effect and could be used for biocontrol.

参考文献/References:

[1] Bérdy J.Thoughts and facts about antibiotics:Where we are now and where we are heading [J].Journal of Antibiotics,2012,65(8):385-395.
[2] 阮继生,黄英.放线菌快速鉴定与系统分类 [M].北京:科学出版社,2011.
[3] 李文均,职晓阳,唐蜀昆.我国放线菌系统学研究历史、现状及未来发展趋势 [J].微生物学通报,2013,40(10):1860-1873.
[4] Goodfellow M,Fiedler H P.A guide to successful bioprospecting:informed by actinobacterial systematics [J].Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology,2010,98(2):119-142.
[5] 刘志恒,王剑,张立新.基因组时代的放线菌系统学及其研究进展 [J].微生物学报,2011,51(2):141-153.
[6] Zhang Yuqin,Liu Hongyu,Yu Liyan,et al.Sinosporangium album gen.nov.,sp.nov.,a new member of the suborder Streptosporangineae [J].International Journal of Systematic and Evolutionary Microbiology,2011,61(4):592-597.
[7] Rong Xiaoying,Guo Yinping,Huang Ying.Proposal to reclassify the Streptomyces albidoflavus clade on the basis of multilocus sequence analysis and DNA-DNA hybridization,and taxonomic elucidation of Streptomyces griseus subsp.Solvifaciens [J].Systematic and Applied Microbiology,2009,32(5):314-322.
[8] Qin Sheng,Xing Ke,Jiang Jihong,et al.Biodiversity,bioactive natural products and biotechnological potential of plant-associated endophytic actinobacteria [J].Applied Microbiology Biotechnology,2011,89(3):457-473.
[9] 田新莉,曹理想,杨国武,等.水稻内生放线菌类群及其对宿主病原菌的抗性研究 [J].微生物学报,2004,44(5):641-646.
[10] Yang Jinghui,Liu Hongxia,Guo Jianhua,et al.Diversity analysis of antagonists from rice-associated bacteria and their application in biocontrol of rice diseases [J].Journal of Applied Microbiology,2008,104(1):91-104.
[11] 中国科学院微生物研究所放线菌分类组.链霉菌鉴定手册 [M].北京:科学出版社,1975.
[12] 沈萍,范秀容,李广武.微生物学实验教程 [M].北京:高等教育出版社,1999.
[13] 阎逊初.放线菌的分类和鉴定 [M].北京:科学出版社,1992.
[14] 张志斌,敖武,熊瑶瑶,等.内生拮抗放线菌FRo2的鉴定及抑菌活性物质的分离 [J].微生物学通报,2014,41(8):1574-1581.
[15] 农旭华,张晓勇,陈茵,等.柳珊瑚共附生放线菌Streptomyces sp. SCSGAA0009 中生物碱类化合物及其抗菌和抗附着活性 [J].微生物学报,2013,53(9):995-1000.
[16] 杨晓楠,李杨,苗建强,等.拮抗放线菌T111菌株鉴定、发酵液理化性质测定及发酵条件优化 [J].应用与环境生物学报,2011,17(4):541-547.
[17] 阮继生.放线菌纲内亚纲、目、亚目、科的 16S rDNA/rRNA 特征序列简介 [J].微生物学通报,2005,32(3):129-134.
[18] 杨平,柴文波,李守淳,等.鄱阳湖微囊藻属形态多样性研究 [J].江西师范大学学报:自然科学版,2014,38(5):496-500.
[18] Chun J,Lee J H,Jung Y,et al.EzTaxon:a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences [J].International Journal of Systematic and Evolutionary Microbiology,2007,57:2259-2261.
[19] Vandamme P,Pot B,Gillis M,et al.Polyphasic taxonomy,a consensus approach to bacterial systematics [J].Microbiology and Molecular Biology Reviews,1996,60(2):407-438.
[20] Shapiro S,Vining L C.Nitrogen metabolism and chloramphenicol production in Streptomyces venezuelae [J].Canadian Journal of Microbiology,1983 [1] Bérdy J.Thoughts and facts about antibiotics:Where we are now and where we are heading [J].Journal of Antibiotics,2012,65(8):385-395.
[2] 阮继生,黄英.放线菌快速鉴定与系统分类 [M].北京:科学出版社,2011.
[3] 李文均,职晓阳,唐蜀昆.我国放线菌系统学研究历史、现状及未来发展趋势 [J].微生物学通报,2013,40(10):1860-1873.
[4] Goodfellow M,Fiedler H P.A guide to successful bioprospecting:informed by actinobacterial systematics [J].Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology,2010,98(2):119-142.
[5] 刘志恒,王剑,张立新.基因组时代的放线菌系统学及其研究进展 [J].微生物学报,2011,51(2):141-153.
[6] Zhang Yuqin,Liu Hongyu,Yu Liyan,et al.Sinosporangium album gen.nov.,sp.nov.,a new member of the suborder Streptosporangineae [J].International Journal of Systematic and Evolutionary Microbiology,2011,61(4):592-597.
[7] Rong Xiaoying,Guo Yinping,Huang Ying.Proposal to reclassify the Streptomyces albidoflavus clade on the basis of multilocus sequence analysis and DNA-DNA hybridization,and taxonomic elucidation of Streptomyces griseus subsp.Solvifaciens [J].Systematic and Applied Microbiology,2009,32(5):314-322.
[8] Qin Sheng,Xing Ke,Jiang Jihong,et al.Biodiversity,bioactive natural products and biotechnological potential of plant-associated endophytic actinobacteria [J].Applied Microbiology Biotechnology,2011,89(3):457-473.
[9] 田新莉,曹理想,杨国武,等.水稻内生放线菌类群及其对宿主病原菌的抗性研究 [J].微生物学报,2004,44(5):641-646.
[10] Yang Jinghui,Liu Hongxia,Guo Jianhua,et al.Diversity analysis of antagonists from rice-associated bacteria and their application in biocontrol of rice diseases [J].Journal of Applied Microbiology,2008,104(1):91-104.
[11] 中国科学院微生物研究所放线菌分类组.链霉菌鉴定手册 [M].北京:科学出版社,1975.
[12] 沈萍,范秀容,李广武.微生物学实验教程 [M].北京:高等教育出版社,1999.
[13] 阎逊初.放线菌的分类和鉴定 [M].北京:科学出版社,1992.
[14] 张志斌,敖武,熊瑶瑶,等.内生拮抗放线菌FRo2的鉴定及抑菌活性物质的分离 [J].微生物学通报,2014,41(8):1574-1581.
[15] 农旭华,张晓勇,陈茵,等.柳珊瑚共附生放线菌Streptomyces sp. SCSGAA0009 中生物碱类化合物及其抗菌和抗附着活性 [J].微生物学报,2013,53(9):995-1000.
[16] 杨晓楠,李杨,苗建强,等.拮抗放线菌T111菌株鉴定、发酵液理化性质测定及发酵条件优化 [J].应用与环境生物学报,2011,17(4):541-547.
[17] 阮继生.放线菌纲内亚纲、目、亚目、科的 16S rDNA/rRNA 特征序列简介 [J].微生物学通报,2005,32(3):129-134.
[18] 杨平,柴文波,李守淳,等.鄱阳湖微囊藻属形态多样性研究 [J].江西师范大学学报:自然科学版,2014,38(5):496-500.
[18] Chun J,Lee J H,Jung Y,et al.EzTaxon:a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences [J].International Journal of Systematic and Evolutionary Microbiology,2007,57:2259-2261.
[19] Vandamme P,Pot B,Gillis M,et al.Polyphasic taxonomy,a consensus approach to bacterial systematics [J].Microbiology and Molecular Biology Reviews,1996,60(2):407-438.
[20] Shapiro S,Vining L C.Nitrogen metabolism and chloramphenicol production in Streptomyces venezuelae [J].Canadian Journal of Microbiology,1983,29(12):1706-1714.
[21] Yang Keqian,Ayer S W.Accumulation of the angucycline antibiotic rabelomycin after disruption of an oxygenase gene in the jadomycin B biosynthetic gene cluster of Streptomyces venezuelae [J].Microbiology,1996,142:123-132.
[22] Xue Yongquan,Sherman D H.Biosynthesis and combinatorial biosynthesis of pikromycin-related macrolides in Streptomyces venezuelae [J].Metabolic Engineering,2001,3(1):15-26.
[23] 杨良,史应武,吴忠红,等.甜瓜细菌性斑点病拮抗菌P-13的鉴定及其抑菌物质的初步研究 [J].微生物学通报,2009,36(6):881-886.
[24] 马林,陈红兵,韩巨才,等.植物内生放线菌Lj20的鉴定及其抗真菌物质的合成 [J].微生物学报,2008,48(7):900-904.
[25] 于妍华,薛泉宏,唐明.西洋参和人参病原真菌菌体对放线菌2种水解酶的诱导 [J].西北农林科技大学学报:自然科学版,2011,39(7):110-115.
[26] 宋金枝.放线菌不同施入方式对连作番茄幼苗生长的影响 [J].西北农业学报,2014,23(2):187-190. ,29(12):1706-1714.
[21] Yang Keqian,Ayer S W.Accumulation of the angucycline antibiotic rabelomycin after disruption of an oxygenase gene in the jadomycin B biosynthetic gene cluster of Streptomyces venezuelae [J].Microbiology,1996,142:123-132.
[22] Xue Yongquan,Sherman D H.Biosynthesis and combinatorial biosynthesis of pikromycin-related macrolides in Streptomyces venezuelae [J].Metabolic Engineering,2001,3(1):15-26.
[23] 杨良,史应武,吴忠红,等.甜瓜细菌性斑点病拮抗菌P-13的鉴定及其抑菌物质的初步研究 [J].微生物学通报,2009,36(6):881-886.
[24] 马林,陈红兵,韩巨才,等.植物内生放线菌Lj20的鉴定及其抗真菌物质的合成 [J].微生物学报,2008,48(7):900-904.
[25] 于妍华,薛泉宏,唐明.西洋参和人参病原真菌菌体对放线菌2种水解酶的诱导 [J].西北农林科技大学学报:自然科学版,2011,39(7):110-115.
[26] 宋金枝.放线菌不同施入方式对连作番茄幼苗生长的影响 [J].西北农业学报,2014,23(2):187-190.

备注/Memo

备注/Memo:
国家自然科学基金(31260137);国家“十二五”重大科技支撑课题(2011BAC13B04);江西省青年科学基金(20132BAB214006);江西省教育厅科研课题(GJJ14250)
更新日期/Last Update: 1900-01-01