ZHAO Xihua,TU Zongcai,WEI Dongzhi,et al.The Recent Advances in Protein Engineering of Cellulases[J].,2015,(04):425-429.





The Recent Advances in Protein Engineering of Cellulases
江西师范大学生命科学学院,江西 南昌,330022;华东理工大学生物工程学院,生物反应器工程国家重点实验室,鲁华生物技术研究所,上海 200237
ZHAO Xihua;TU Zongcai;WEI Dongzhi;WANG Wei;TANG Pingping
cellulaseprotein engineeringrecent advances
Parent cellulases exist in some defects of a low catalytic activity,inhibition of end products,weak thermo-stability,and poor processivity,and therefore improving enzymatic properties of cellulases is a very meaningful job. To date,on the basis of the technology of evolution in vitro,it is an effective way to develop enzymatic properties of cellulases. Recent advances in protein engineering about three different regions of cellulases which consist of catalyt-ic domains,binding domains and linker regions are reviewed in the paper. The idea about systematically assembling functional structure domains which have been engineered was put forward to in the paper,which will help to improve the enzymatic properties such as catalytic activity,the ability of processive hydrolysis,resistantance to high concen-tration of product,and thermalstability,and to promote high-efficent application in energy,industry,agriculture, food,and so on.


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更新日期/Last Update: 1900-01-01