[1]周永丹,张勇,李存雄,等.HPLC法同步测定谷物中的吡虫啉和辛硫磷[J].江西师范大学学报(自然科学版),2015,(06):566-569.
 ZHOU Yongdan,ZHANG Yong,LI Cunxiong,et al.The Determination of Imidacloprid and Phoxim Residues in Cereals by HPLC[J].,2015,(06):566-569.
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HPLC法同步测定谷物中的吡虫啉和辛硫磷()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
566-569
栏目:
出版日期:
2015-12-31

文章信息/Info

Title:
The Determination of Imidacloprid and Phoxim Residues in Cereals by HPLC
作者:
周永丹;张勇;李存雄;陈顺琴;吴浪
1.贵州师范大学化学与材料科学学院,贵州 贵阳 550001; 2.贵州师范大学贵州省山地环境信息系统和生态环境保护重点实验室,贵州 贵阳 550001; 3.贵州师范学院化学与生命科学学院,贵州 贵阳 550018
Author(s):
ZHOU YongdanZHANG YongLI CunxiongCHEN ShunqinWU Lang
1.College of Chemistry and Material Science,Guizhou Normal University,Guiyang Guizhou 550001,China; 2.Key Laboratory for Information System of Mountainous Area and Protection of Protection of Ecological Environment of Guizhou Province, Guizhou Normal University,Guiyang Guizhou 550001,China; 3.College of Chemistry and Life Science,Guizhou Normal College,Guiyang Guizhou 550018,China
关键词:
高效液相色谱 谷物 吡虫啉 辛硫磷
Keywords:
high-performance liquid chromatography grain imidacloprid phoxim
分类号:
TQ 450
文献标志码:
A
摘要:
建立了高效液相色谱法(HPLC)同步测定大米、荞麦、玉米等谷物中吡虫啉与辛硫磷残留量的方法.样品用乙腈提取经硅镁型吸附剂净化,以w(乙腈):w(水)=80:20作为流动相,高效液相色谱(配紫外检测器-UVD)分析,检测波长为280 nm.结果表明:该方法对谷物中吡虫啉与辛硫磷的检出限(LOD)分别为0.01 mg·kg和0.02 mg·kg,均符合相关要求; 标准品浓度在0.05~5.00 μg·mL之间时线性关系良好,相关系数分别为0.999 6和0.999 8.在0.10,0.20,0.40 mg·kg的3个水平浓度下回收率范围为81.3%~96.7%,相对标准偏差(RSD)为0.9%~4.7%.该方法具有简单、快捷、准确等优点,适合谷物中吡虫啉与辛硫磷的同步测定.
Abstract:
An efficient method with high performance liquid chromatography(HPLC)was established to detect the imidaclorprid and phoxim redidues in cereals(rice,buckwheat,corn,etc).The samples were extracted by methyl cyanide and prurified by florisil.Using methyl cyanide and water as mobile phase,samples were analysed by HPLC with UVD,detection wavelength of 280 nm.Test results using the methond applied in imidacloprid and phoxime can be described as follows:imidacloprid limit of detection(LOD)was 0.01 mg·kg, phoxime LOD was 0.02 mg·kg.There is a good linear relationship when the concentration is in the range of 0.05~5.00 μg·mL,the correlation coefficient of 0.999 6 and 0.999 8.The recoveries fluctuated in the range of 81.3%~96.7% for different levels(0.10,0.20,0.40 mg·kg),the relative standard deviations(RSD)were 0.9%~4.7%.Simplicity,efficiency and accuracy are the key qualities of this method which is suitable for determination of imidacloprid and phoxim residues in cereals.

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相似文献/References:

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 WU Lang,ZHANG Yong,LI Cunxiong,et al.The Determination of Carbendazim Residue in Buckwheat by HPLC[J].,2015,(06):246.

备注/Memo

备注/Memo:
基金项目:贵州省科技厅科学技术基金(黔科合J字LKS
[2011]30号)和贵州省教育厅招标(黔教科(2011)018号)资助项目.
更新日期/Last Update: 1900-01-01