[1]李希茜,颜日明,张志斌,等.内生真菌SF4的鉴定及产乙酰胆碱酯酶抑制剂发酵条件的初步优化[J].江西师范大学学报(自然科学版),2016,40(06):569-573.
 LI Xixi,YAN Riming,ZHANG Zhibin,et al.The Identification and Optimization of Fermentation Conditions of Acetylcholinesterase Inhibiters-Producing Endophytic Fungi Strain SF4[J].,2016,40(06):569-573.
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内生真菌SF4的鉴定及产乙酰胆碱酯酶抑制剂发酵条件的初步优化()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
40
期数:
2016年06期
页码:
569-573
栏目:
出版日期:
2016-12-01

文章信息/Info

Title:
The Identification and Optimization of Fermentation Conditions of Acetylcholinesterase Inhibiters-Producing Endophytic Fungi Strain SF4
作者:
李希茜颜日明张志斌杨慧琳朱 笃汪 涯
1.江西中医药大学科技学院,江西 南昌 330025; 2.江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西 南昌 330022; 3.江西科技师范大学生命科学学院,江西省生物加工过程重点实验室,江西 南昌 330013; 4.宜春学院江西省天然药物活性成分研究重点实验室,江西 宜春 336000
Author(s):
LI XixiYAN RimingZHANG ZhibinYANG HuilinZHU DuWANG Ya
1.Science and Technology College,Jiangxi University of Traditional Chinese Medicine,Nanchang Jiangxi 330025,China; 2.Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province,Jiangxi Normal University,Nanchang Jiangxi
关键词:
内生真菌 乙酰胆碱酯酶抑制剂 乙酰胆碱酯酶 鉴定 发酵
Keywords:
endophytic fungi acetylcholinesterase inhibiters acetylcholinesterase identification fermentation
分类号:
R 284.2
摘要:
基于菌株菌落和显微形态特征以及ITS-rDNA序列分析结果,将产乙酰胆碱酯酶抑制剂(AChEI)的内生真菌SF4鉴定为枝顶孢霉属Acremonium sp.真菌,命名为Acremonium sp.SF4.通过对菌株SF4发酵的基础培养基、发酵温度、发酵时间、摇床转速进行初步优化,确定了其最适产AChEI的摇瓶发酵条件为:沙氏培养基,发酵温度28 ℃,摇床转速为150 r·min-1,发酵培养14 d.在此条件下可发酵获得含AChEI的发酵浸膏0.331 g·L-1,对乙酰胆碱酯酶的抑制率可达60.27%,相比优化前均有提高.研究有助于后期规模化高效发酵,为分离、纯化和结构解析单一组分AChEI奠定基础.
Abstract:
The acetylcholinesterase inhibiters(AChEI)-producing endophytic fungi strain SF4 was identified as Acremonium sp.SF4 based on its morphological characteristics and ITS-rDNA sequence analysis.The effects of fermentation medium,temperature,rotation speed and cultural time on acetylcholinesterase(AChE)inhibitory activity and AChEI metabolites amount were analyzed.It was indicated that the appropriate fermentation condition was:sabouraud dextrose broth(SDB)medium,28 ℃,150 r·min-1 for 14 d.Under the optimized conditions of fermentation,the amount of AChEI metabolites and AChE inhibitory activitycytotoxic activity is 0.331 g·L-1,and 60.27% respectively,higher than initial conditions.These results can lay the foundation for the further separation and purification of the AChEI metabolites.

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备注/Memo

备注/Memo:
收稿日期:2016-06-01基金项目:国家自然科学基金(81260617,31300051)和江西省自然科学基金(20142BAB214020)项目资助.通信作者:汪 涯(1986-),男,江西弋阳人,讲师,主要从事微生物与天然产物领域研究.
更新日期/Last Update: 1900-01-01