[1]陈云芳,王 胜,钱自亮.香烟烟雾法肺动脉高压小鼠模型肺组织HIF-1α和Nrf2表达[J].江西师范大学学报(自然科学版),2017,(02):155-159.
 CHEN Yunfang,WANG Sheng,QIAN Ziliang.The Expression of HIF - 1α and Nrf2 in the Lung of Pulmonary Arterial Hypertension Mice[J].,2017,(02):155-159.
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香烟烟雾法肺动脉高压小鼠模型肺组织HIF-1α和Nrf2表达()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年02期
页码:
155-159
栏目:
出版日期:
2017-03-01

文章信息/Info

Title:
The Expression of HIF - 1α and Nrf2 in the Lung of Pulmonary Arterial Hypertension Mice
作者:
陈云芳王 胜钱自亮
1.北京大学深圳研究生院化学院,广东 深圳 518055; 2.广州医科大学附属第一医院研究所,广州呼吸疾病研究所,广东 广州 510120; 3.中山大学第八附属医院,广东 深圳 518033
Author(s):
CHEN YunfangWANG ShengQIAN Ziliang
1.Peking University Shenzhen Graduate School,Shenzhen Guangdong 518055,China; 2.The First Affiliated Hospital of Guangzhou Medical University,Guangzhou Institute Of Respiratory Diseases,Guangzhou Guangdong 510120,China; 3.The Eight Affiliated Hospital,S
关键词:
肺动脉高压 吸烟 低氧诱导因子1α 转录因子NF-E2相关因子2
Keywords:
pulmonary arterial hypertension cigarette HIF-1α Nrf2
分类号:
Q 813; R 73-3
文献标志码:
A
摘要:
以香烟烟雾熏烟法建立肺动脉高压(PAH)小鼠模型,将C57B6J小鼠随机分为对照组和模型组,每组10只,对照组小鼠不作任何处理,模型组采用香烟处理:每天2次,每次2 h,10支/h,6 d/周,连续6个月的建模方法; 使用小鼠右心室压力测定仪(BIOPAC Systems,MP150)测定小鼠右心室压力(RVSP); 将肺小动脉进行胶原纤维染色(Van-Gieson法),结合Image Pro Plus软件分析,测量肺小动脉血管的中层壁厚和肺小动脉血管的外径,计算肺小动脉厚度的变化; 利用Western Blot法检测Nrf2和HIF-1α蛋白在肺组织的表达.结果表明:1)与对照组相比,模型组小鼠右心室压力明显增加,肺小动脉血管壁明显增厚,管腔变狭窄,右心室与体重的比值显著增加; 2)与对照组相比,模型组小鼠肺组织HIF-1α蛋白表达明显增加,而Nrf2蛋白表达明显减少.研究结果意味着:HIF-1α与Nrf2在香烟烟雾法建立的肺动脉高压小鼠肺组织中表达异常.
Abstract:
Use cigarette smoke to establish pulmonary hypertension(PAH)mice model,C57B6J mice were randomly divided into control group and model group,each group of 10,control group don’t make any processing,model group deal with cigarette:2 times a day,every time 2 h,10 piece/h,6 days/week,stick to 6 months to establish model; Use the mouse right ventricular pressure tester(BIOPAC Systems,MP150)measure right ventricular pressure(RVSP)of mice; Use pulmonary arteriole make collagen fiber staining(Van Gieson method),combined with the Image Pro Plus software analysis,Measure pulmonary small arteries middle wall thickness and pulmonary small artery diameter,calculate the thickness of pulmonary arterioles change; Use Western Blot method to detect Nrf2 and HIF-1α protein expression in lung tissue.The results showed that:1)Compared with control mice,the right ventricular systolic pressure was increased significantly,the thickening of lung small artery wall and pulmonary stenosis were occurred,and the ratio of right ventricular /weight was significantly increased in model mice.2)The expression of HIF-1α was significantly increased,while the in the expression of Nrf2 was significantly reduced in model mice compared with control mice.The results mean:HIF-1α and Nrf2 had an abnormal expression in PAH mice.

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备注/Memo

备注/Memo:
收稿日期:2016-10-15基金项目:国家自然科学基金(30971166)和深圳市科技计划基础研究(JCYJ20140416094256880)资助项目.通信作者:钱自亮(1964-),男,湖南隆回人,主任医师,博士,主要从事疼痛的机制与治疗研究.E-mail:Ziliangqian16@163.com
更新日期/Last Update: 1900-01-01