[1]王雪锋,王亚丽,赵文霞,等.悬浮溶剂固化-分散液液微萃取法提取小米中β-胡萝卜素[J].江西师范大学学报(自然科学版),2018,(04):422-426.[doi:10.16357/j.cnki.issn1000-5862.2018.04.18]
　WANG Xuefeng,WANG Yali,ZHAO Wenxia,et al.The Extraction of β-Carotene in Millet Using Dispersive Liquid-Liquid Microextraction Combined with Solidified Floating Organic Droplets[J].Journal of Jiangxi Normal University:Natural Science Edition,2018,(04):422-426.[doi:10.16357/j.cnki.issn1000-5862.2018.04.18]
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悬浮溶剂固化-分散液液微萃取法提取小米中β-胡萝卜素()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年04期
页码:
422-426
栏目:
出版日期:
2018-08-20

文章信息/Info

Title:
The Extraction of β-Carotene in Millet Using Dispersive Liquid-Liquid Microextraction Combined with Solidified Floating Organic Droplets
文章编号:
1000-5862(2018)04-0422-05
作者:
王雪锋1; 2; 王亚丽1; 2; 赵文霞1; 2; 闫海彦1; 曹江平1; 2*; 邸宏伟1; 2; 梁永锋1; 2; 解启龙3
1.宁夏师范学院化学化工学院,宁夏 固原 756000; 2.宁夏师范学院六盘山资源工程技术研究中心,宁夏 固原 756000; 3.中国科学院山西煤炭化学研究所煤转化国家重点实验室,山西 太原 030001
Author(s):
WANG Xuefeng1; 2; WANG Yali1; 2; ZHAO Wenxia1; 2; YAN Haiyan1; CAO Jiangping1; 2*;  DI Hongwei1; 2; LIANG Yongfeng1; 2; XIE Qilong3
1.College of Chemistry and Chemical Engineering,Ningxia Normal University,Guyuan Ningxia 756000,China; 2.Engineering and Technology Research Center of Liupanshan Resources,Ningxia Normal University,Guyuan Ningxia 756000,China; 3.State Key Laboratory of
关键词:
悬浮溶剂固化;  分散液液微萃取;  β-胡萝卜素
Keywords:
solidified floating organic droplets;  dispersive liquid-liquid microextraction;  β-carotene
分类号:
O 657
DOI:
10.16357/j.cnki.issn1000-5862.2018.04.18
文献标志码:
A
摘要:
建立了悬浮溶剂固化-分散液液微萃取法提取小米中β-胡萝卜素的方法,并用紫外-可见分光光度计测定其含量.结果表明,最佳提取条件是:取4 mL小米样品的皂化液,用5 mL蒸馏水稀释到10 mL离心管中,调节pH值为7、加入NaCl至饱和,快速加入混匀的200 μL十二醇和200 μL乙腈溶液萃取,完成后将离心管迅速置于冰水浴中 静置5 min,用微量注射器吸出残余样品液后,室温融化固化的萃取剂用四氢呋喃溶解并定容至1 000 μL.萃取完成后将提取液用紫外-可见分光光度计在波长460 nm处测定小米中β-胡萝卜素含量.该方法工艺简单,提取率高.
Abstract:
A novel method for the extraction and determination of β-carotene in millet has been developed by dispersive liquid-liquid microextraction combined with solidified floating organic droplets(SFO-DLLME)and ultraviolet visible spectrophotometer.The results show that the optimal parameters are diluting 4 mL of saponified solution with 5 mL water,adjusting pH=7,adding saturated NaCl and 200 μL 1-dodecanol(extractant)with the same volume of acetonitrile(dispersant)to the aqueous solution for extracting.After that,the centrifuge tube is immersed in an ice-water bath for 5 min,the floated organic solvent is solidified on top of the surface of the aqueous solution after a short period of time,and any liquid phase is removed using the same syringe.The solidified floating is quickly melted at room temperature and the extract is diluted to 1 000 μL using THF.Ultimately,β-carotene in the extract is determined by ultraviolet visible spectrophotometer.The advantage of the extraction method shows simplicity of the procedure,high extraction rate,which is available for the extraction and determination of β-carotene in millet.

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备注/Memo

备注/Memo:
收稿日期:2017-12-29
基金项目:国家自然科学基金(21561027),宁夏科技创新领军人才基金(KJT2016004),宁夏师范学院校级科研(NXSFZDA1812,NXSFZD1715)和宁夏师范学院六盘山资源工程技术研究中心(HG18-04)资助项目.
通信作者:曹江平(1984-),男,陕西陇县人,助教,主要从事分离分析新技术及其在食品、环境分析领域的应用研究.E-mail:jiangpingcao@126.com
更新日期/Last Update: 2018-08-20