[1]谢茹胜,李 蒙,龙敏南.大米草半纤维素选择性酶解研究[J].江西师范大学学报(自然科学版),2020,(05):501-505.[doi:10.16357/j.cnki.issn1000-5862.2020.05.09]
　XIE Rusheng,LI Meng,LONG Minnan.The Study on Selective Enzymatic Hydrolysis of Hemicellulose from Spartina anglica[J].Journal of Jiangxi Normal University:Natural Science Edition,2020,(05):501-505.[doi:10.16357/j.cnki.issn1000-5862.2020.05.09]
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大米草半纤维素选择性酶解研究()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年05期
页码:
501-505
栏目:
生命科学
出版日期:
2020-10-20

文章信息/Info

Title:
The Study on Selective Enzymatic Hydrolysis of Hemicellulose from Spartina anglica
文章编号:
1000-5862(2020)05-0501-05
作者:
谢茹胜1; 李 蒙2; 龙敏南1
1.福建生物工程职业技术学院药学系,福建 福州 350002; 2.厦门大学能源学院,福建 厦门 361102
Author(s):
XIE Rusheng1; LI Meng1; 2; LONG Minnan1; 2
1.Fujian Vocational College of Bioengineering,Fuzhou Fujian 350002,China; 2.College of Energy,Xiamen University,Xiamen Fujian 361102,China
关键词:
大米草半纤维素;  木聚糖酶;  东方肉座菌EU7-22;  黑曲霉BE-2;  酶降解
Keywords:
hemicellulose of Spartina anglica;  xylanase;  Hypocreaorientalis EU7-22;  Aspergillusniger BE-2;  enzymic degradation
分类号:
TQ 35
DOI:
10.16357/j.cnki.issn1000-5862.2020.05.09
文献标志码:
A
摘要:
采用由东方肉座菌EU7-22表达的重组木聚糖酶HoXyn11A、黑曲霉BE-2表达的重组木聚糖酶AnXyn10C和商品木聚糖酶分别降解大米草半纤维素,探索其降解的最佳工艺条件,结果发现该3种木聚糖酶最适pH值为4.5～5.5,最佳反应温度为45～55 ℃,酶用量为200～300 IU·g-1底物,反应时间为12～24 h.对比结果表明:通过黑曲霉BE-2表达的重组木聚糖AnXyn10C能够在24 h内基本将大米草半纤维素降解为低聚木糖,效率远高于商品酶和东方肉座菌EU7-22表达的重组木聚糖酶HoXyn11A.黑曲霉BE-2表达的重组木聚糖AnXyn10C是大米草半纤维素降解酶的良好来源.
Abstract:
The xylanase enzyme including xylanase HoXyn11A produced from Hypocreaorientalis EU7-2,xylanase AnXyn10C produced from Aspergillusniger BE-2 and commodity xylanase are respectively used to degrade hemicellulose of Spartina anglica.And its best condition of process is also explored.The result shows that the most suitable pH value of the 3 species enzyme is from 4.5 to 5.5,the best reaction temperature is from 45 ℃ to 55 ℃,the suitable enzyme dosage is 200 IU·g-1 to 300 IU·g-1 substrate,the best reaction time is 12 h to 24 h.The comparison results show that the recombinant xylan AnXyn10C expressed by aspergillus Niger b-2 can degrade hemicellulose into xylose oligosaccharide within 24 h,with a much higher efficiency than commercial enzymes and xylanase HoXyn11A produced from Hypocreaorientalis EU7-2.Hence,the recombinant xylan AnXyn10C expressed by Aspergillusniger BE-2 b-2 is an excellent source of enzymatic degradation of hemicellulose from Spartina anglica,which have great significance.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2020-02-27
基金项目:闽海洋高新“海洋生物制品创新服务平台”[201621]资助项目.
作者简介:谢茹胜(1978-),女,福建漳州人,副教授,主要从事化学、海洋生物制品研究.E-mail:xierusheng78@163.com
更新日期/Last Update: 2020-10-20