[1]黎小军,谢莲萍,刘建宏,等.产脂肪酶菌株的筛选、鉴定与产酶条件优化[J].江西师范大学学报(自然科学版),2014,(01):14-18.
　LI Xiao-jun,XIE Lian-ping,LIU Jian-hong,et al.The Screening and Identification of a Lipase-Producing Strain and Its Culture Condition Optimization[J].Journal of Jiangxi Normal University:Natural Science Edition,2014,(01):14-18.
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产脂肪酶菌株的筛选、鉴定与产酶条件优化()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2014年01期
页码:
14-18
栏目:
出版日期:
2014-02-28

文章信息/Info

Title:
The Screening and Identification of a Lipase-Producing Strain and Its Culture Condition Optimization
作者:
黎小军;谢莲萍;刘建宏;朱婷婷;刘蓉
新余学院医学与生命科学学院,江西新余,338000
Author(s):
LI Xiao-jun；XIE Lian-ping；LIU Jian-hong；ZHU Ting-ting；LIU Rong
关键词:
脂肪酶; 洋葱假单胞菌; 筛选; 鉴定; 优化
Keywords:
lipase; Burkholderia cepacia; screening; identification; optimization
分类号:
Q939；TQ925
文献标志码:
A
摘要:
以橄榄油为唯一碳源,采用平板变色圈法,从土壤中筛选到1株脂肪酶高产菌XYU-6,经生理生化试验鉴定及16S rDNA序列分析,XYU-6被鉴定为洋葱假单胞菌(Burkholderia cepacia).设计单因素试验,对其产酶条件进行优化,优化后的培养条件为:葡萄糖1.00；,蛋白胨2.00；,豆油1.00；,KH2 PO40.35；,K2 HPO4 0.15；,MgSO40.05；,初始pH值为7.0,培养温度30℃,培养48 h,酶活可达24.1U·mL-1,是优化前的1.65倍.
Abstract:
A lipase producing strain XYU-6 was isolated from the soil by the method of flat discolor circle with olive oil as sole carbon source.XYU-6 was identified as Burkholderia cepacia by 16S rDNA sequencing and its biochemical and physiological characteristics.The optimal conditions,glucose 1.0%,peptone 2.0%,soya-bean oil 1%,KH2PO4 0.35%,K2HPO4 0.15% and MgSO4 0.05% were obtained through one-factor experimental design.Furthermore,the highest lipase activity 24.1 U·mLwas achieved under the optimum conditions with initial pH 7.0,culture temperature 30 ℃ and culture time 48 h.These results indicated that the lipase activity was improved 1.65 fold compared to the original conditions.

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相似文献/References:

[1]黎小军,林陈水.Burkholderia cepacia XYU-6脂肪酶的克隆及其细胞表面展示[J].江西师范大学学报(自然科学版),2015,(05):502.
　LI Xiaojun,LIN Chenshui.The Cloning and Cell Surface Display of a Lipase from Burkholderia cepacia XYU-6[J].Journal of Jiangxi Normal University:Natural Science Edition,2015,(01):502.

备注/Memo

备注/Memo:
江西省青年科学基金(20122BAB214011)
更新日期/Last Update: 1900-01-01