[1]杨成彬,闫浩,刘晓宇,等.花生过敏小鼠腹腔肥大细胞SOC活性与ROS相关性研究[J].江西师范大学学报(自然科学版),2012,(01):29-34.
　YANG Cheng-bin,YAN Hao,LIU Xiao-yu,et al.The Experiment Study of Peritoneal Mast Cell SOC Activity of Mice That Have Been Therapied by Antioxidant[J].Journal of Jiangxi Normal University:Natural Science Edition,2012,(01):29-34.
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花生过敏小鼠腹腔肥大细胞SOC活性与ROS相关性研究()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2012年01期
页码:
29-34
栏目:
出版日期:
2012-01-01

文章信息/Info

Title:
The Experiment Study of Peritoneal Mast Cell SOC Activity of Mice That Have Been Therapied by Antioxidant
作者:
杨成彬;闫浩;刘晓宇;夏立新;李荔;刘杰;刘志刚
1. 深圳大学过敏反应与免疫学研究所, 广东 深圳 518060; 2.深圳大学医学院, 广东 深圳 518060
Author(s):
YANG Cheng-bin;  YAN Hao;  LIU Xiao-yu;  XIA Li-xin;  LI Li;  LIU Jie;  LIU Zhi-gang
关键词:
花生过敏;  肥大细胞;  SOC;  ROS
Keywords:
peanut allergy;  mast cell;  SOC;  ROS
分类号:
Q 785
文献标志码:
A
摘要:
研究花生过敏状态下的C57BL/6小鼠腹腔肥大细胞的激活与钙离子通道之间的内在联系, 以及探讨肥大细胞钙离子通道与ROS(reactive oxygen species)之间的关系. 18只雌性C57BL/6小鼠随机分为PBS组(A组)、花生过敏模型组(B组)、vitamin A和vitamin E灌胃治疗组(C组). 酶联免疫吸附实验检测各组腹腔上清液中特异性的IgE和IgG2a水平, 以及细胞因子的变化, 另外测定腹腔上清液与血清中的组胺、ROS含量. 腹腔灌洗液中细胞涂片, 进行肥大细胞计数和脱颗粒比例统计, 荧光共聚焦显微镜观察腹腔肥大细胞钙离子通道SOC(Store oprated channel)的活性. 腹腔上清液中花生特异性IgE、IL-4、IL-10、组胺、ROS、血清中的组胺和ROS、腹腔灌洗液中肥大细胞总数、肥大细胞脱颗粒比例, 都呈现出A组
Abstract:
By establish mice peanut allergy and antioxidant therapy allergy mice model, to investigated correlation activation of mast cell with SOC channel, and further to study the relationship between mast cell SOC and ROS. 18 female C57BL/6 mice were divided into three groups that one is treated by PBS and the other is treated by lavaging crude peanut protein, another is peanut allergy mice that been therapied by lavaging vitamin A and vitamin E. Peanut protein specific IgE and IgG2a, cytokines, histamin and ROS in supernatant of peritoneal lavaging were assayed by enzyme-linked immunoassay(ELISA). In addition, hiatamin and ROS in blood serum were also assayed by ELISA. By peritoneal lavaging, inflammatory cells and mast cell were anlalyzed. Confocal was used to detect SOC activity of peritoneal mast cell. In peritoneal lavaging experiment, the total numbers of cells, eosinophil, mast cell of B group were greatly increased than A group, but these numbers of C groups were lower than B group. The levels of IgE, IL-4,IL-10 in supernatant of peritoneal lavaging liquid were presented a trendency, which C group were lower than B group, but higher than A group. But the levels of IgG2a, 1L-6,IL-10 were differences, which C group were lower than A group, but higher than B group. The levels of histamin and ROS presented a trendency, which C group were lower than B group, but higher than A group. Confocal recorded the fluorescence intensity of intracellular calcium changes. The datas of confocal recorded show that SOC activity of B groups mast cells were increased, but contrast to B group, SOC activity of C group were inhibited. Vitamin A and vitamin C can relieve allergy induced by peanut crud protein. However antioxidant can inhibit SOC activity of mast cell by scavenge ROS. Accordingly antioxidant depressed mast cell degranulation and then relieve anaphylactic response. This study laid the foundation for further research in the mechanism of antioxidants treatment of peanut allergy, while it has some significance for the clinical treatment of anaphylaxis.

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更新日期/Last Update: 1900-01-01