[1]林春花.超高效合相色谱-质谱法测定大豆油中5种脂肪酸含量[J].江西师范大学学报(自然科学版),2015,(02):162-165.
　LIN Chunhua.The Detection of Five Fatty Acids in Soybean Oil by Ultra Performance Convergence Chromatography-Mass Spectrometry[J].Journal of Jiangxi Normal University:Natural Science Edition,2015,(02):162-165.
点击复制

超高效合相色谱-质谱法测定大豆油中5种脂肪酸含量()
分享到：

《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
162-165
栏目:
出版日期:
2015-04-10

文章信息/Info

Title:
The Detection of Five Fatty Acids in Soybean Oil by Ultra Performance Convergence Chromatography-Mass Spectrometry
作者:
林春花
江西师范大学国家单糖化学合成工程技术研究中心,江西 南昌 330027
Author(s):
LIN Chunhua
关键词:
超高效合相色谱-质谱法;  大豆油;  脂肪酸;  未衍生化
Keywords:
ultra-performance convergence chromatography-mass spectrometry;  soybean oil;  fatty acids;  underivatization
分类号:
O 657.63
文献标志码:
A
摘要:
建立了大豆油中5种脂肪酸的超高效合相色谱-质谱(UPC-MS)分析方法,这5种脂肪酸分别为棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸.待测物经皂化反应后,用正己烷溶解,经ACQUITY UPC BEH 2-EP色谱柱分离,以CO2-甲醇/乙腈(体积比为1:1)为流动相进行梯度洗脱,通过质谱检测器测定、外标法定量.方法的最低检出限为0.07 mg·L,脂肪酸的加标回收率为90.50；～105.38；,相对标准偏差为0.81；～2.93；.结果表明,该方法灵敏度高、分离效果好、测定结果准确.
Abstract:
The 5 fatty acids in soybean oil were analyzed using ultra-performance convergence chromatography,including palmitic acid,stearic acid,oleic acid,linoleic acid and linolenic acid.The sample was dissolved by n-hexane after saponification reaction.The fatty acids were separated on the column of ACQUITY UPC BEH 2-EP by gradient elution with carbon dioxide and V(methano):V(acetonitrile)=1:1 system,and finally detected by MS detector.The limits of detection of target compounds in the method were 0.07 mg·L.The recoveries were from 90.50； to 105.38； at three spiked levels with the relative standard deviations lower than 3.0；.The method,with high sensitivity,good separation effect and high recovery,was successfully used to detect the underivatization fatty acids in soybean oil.

参考文献/References:

[1] 穆同娜,孙婷,吴燕涛,等.3种食用植物油中不饱和脂肪酸含量调查 [J].粮油食品科技,2011,3(19):36-38.
[2] 曾建立,杜泽学,陈艳凤.气相色谱分析未衍生化脂肪酸及其甲酯 [J].石油炼制与化工,2012,43(7):104-109.
[3] 蒋秀琴,刘立成,赵福忠,等.常见植物油脂肪酸含量的分析 [J].饲料博览,2010(3):27-30.
[4] 郑月明,冯峰,国伟,等.全2维气相色谱-四极杆质谱法检测植物油脂中脂肪酸 [J].色谱,2012,11(30):1166-1171.
[5] 朱笃,徐曲.青葙籽油脂肪酸组成的分析 [J].江西师范大学学报:自然科学版,2002,26(2):110-112.
[6] 回瑞华,候冬岩,李铁纯,等.棉籽油中脂肪酸不同的酯化方法与气相色谱-质谱分析 [J].质谱学报,2005,26(2):90-92.
[7] 徐桂转,梁新,苏惠,等.利用气相色谱分析生物柴油中脂肪酸甲酯含量研究 [J].安徽农业科学,2008,36(28):12090-12091.
[8] Ghiaci M,Aghabarari B,Gil A.Production of biodiesel by esterification of natural fatty acids over modified organoclay catalysts [J].Fuel,2011,90(11):3382-3389.
[9] 巫淼鑫,邬国英,韩瑛,等.6种食用植物油及其生物柴油中脂肪酸成分的比较研究 [J].中国油脂,2003,28(12):65-67.
[10] 张大华,李丽霞.液相色谱法测定生物柴油中多种脂肪酸甲酯 [J].粮食与油脂,2007(12):41-43.
[11] Wood R,Lee T.High-performance liquid chromatography of fatty acids:Quantitative analysis of saturated,monoenoic,polyenoic and geometrical isomers [J].Journal of Chromatography A,1983,254:237-246.
[12] 佘珠花.气相色谱法中油脂脂肪酸衍生化方法及其选择 [J].粮食加工,2004(6):64-66.
[13] 孟哲,文大为,廖一平,等.快速气相色谱法测定食用油中的为衍生化长链脂肪酸 [J].色谱,2007,25(2):254-257.
[14] 李一哲,包桂蓉,王华.超高效液相色谱法测定生物柴油中的11种脂肪酸及其甲酯 [J].色谱,2008,26(4):494-498.
[15] 徐永威,孙庆龙,黄静,等.Waters ACQUITY UPC仪器结构和性能特点 [J].现代仪器,2012,18(5):45-48.
[16] 邹洁,赵维佳,汪海峰,等.大豆油中掺伪棕榈油的检测方法研究 [J].中国油脂,2009,34(04):73-76.

备注/Memo

备注/Memo:
国家科技支撑计划(2012BAE07B00)
更新日期/Last Update: 1900-01-01