[1]汤丽娟,包建莹,颜日明,等.不同碳源对内生真菌Shiraia sp. Slf14产苝醌类色素的影响[J].江西师范大学学报(自然科学版),2019,(05):490-495.[doi:10.16357/j.cnki.issn1000-5862.2019.05.09]
　TANG Lijuan,BAO Jianying,YAN Riming,et al.The Effects of Different Carbon Sources on Production of Perylenequinones in Endophytic Fungus Shiraia sp. Slf14[J].Journal of Jiangxi Normal University:Natural Science Edition,2019,(05):490-495.[doi:10.16357/j.cnki.issn1000-5862.2019.05.09]
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不同碳源对内生真菌Shiraia sp. Slf14产苝醌类色素的影响()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年05期
页码:
490-495
栏目:
出版日期:
2019-10-10

文章信息/Info

Title:
The Effects of Different Carbon Sources on Production of Perylenequinones in Endophytic Fungus Shiraia sp. Slf14
文章编号:
1000-5862(2019)05-0490-06
作者:
汤丽娟1; 包建莹1; 颜日明1; 汪 涯2; 杨慧林1; 张志斌1; 朱 笃1; 2*
1.江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西 南昌 330022; 2.江西科技师范大学生命科学学院,江西省生物加工过程重点实验室,江西 南昌 330013
Author(s):
TANG Lijuan1; BAO Jianying1; YAN Riming1;  WANG Ya2; YANG Huilin1; ZHANG Zhibin1; ZHU Du1; 2*
1.Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province,College of Life Sciences,Jiangxi Normal University,Nanchang Jiangxi 330022,China; 2.Key Laboratory of Bioprocess of Jiangxi Province,College of Life Sciences,Jiangxi Science and Technology Normal University,Nanchang Jiangxi 330013,China
关键词:
竹黄菌;  碳源;  苝醌类化合物;  竹红菌素;  代谢调控
Keywords:
Shiraia bambusicola;  carbon sources;  perylenequinones;  hypocrellins;  metabolic regulation
分类号:
S 326
DOI:
10.16357/j.cnki.issn1000-5862.2019.05.09
文献标志码:
A
摘要:
苝醌类化合物是一类具有抗癌、抗病毒活性的光敏性色素.该文比较了不同碳源对蛇足石杉内生真菌Shiraia sp. Slf14液态发酵产苝醌类化合物的影响,结果表明:可溶性淀粉有利于Shiraia sp. Slf14菌体的生长,而果糖则有利于苝醌类化合物的生物合成.当果糖浓度为60 g·L-1时,菌体苝醌类化合物总产量可达1 566.64 mg·L-1,同时果糖能提升竹红菌甲素组分的含量.菊粉作为一种来源于菊芋的果糖基生物质,同样有利于苝醌类色素的生物合成,当其浓度为40 g·L-1时,色素总产量可达1 322.85 mg·L-1,且其有利于痂囊腔菌素A组分积累.
Abstract:
Perylenequinones(PQ)are a class of photosensitive pigments with antitumor and antiviral activity.In present study,the effects of different carbon sources on the growth and PQ production of the endophytic fungus Shiraia sp.Slf14 are studied.The results show that soluble starch is suitable to fungal growth,while fructose can enhance PQ production significantly.With 60 g·L-1 fructose as the carbon source,the total PQ yield of 1 566.64 mg·L-1 is achieved.Moreover,fructose is also conducive to the accumulation of hypocrellin A(HA)component.Besides,the inulin as a kind of fructose based biomass from Jerusalem artichoke also could improve PQ production.By using 40 g·L-1 inulin,the total PQ yield of 1 322.85 mg·L-1 is obtained,and inulin could enhance the accumulation of elsinochrome A(EA)component.The results of this study will lay a basis for improving PQ fermentation production of Shiraia sp.Slf14.

参考文献/References:

[1] Mulrooey C A,O’Brien E M,Morgan B J,et al.Perylenequinones:isolation,synthesis,and biological activity[J].European J Org Chem,2012,2012(21):3887-3904.
[2] Zhenjun Diwu,Lown J W.Hypocrellins and their use in photosensitization[J].Photochem Photobiol,1990,52(3):609-616.
[3] 梁晓辉,蔡宇杰,廖祥儒,等.药用真菌竹黄的研究进展[J].食品与生物技术学报,2008,27(5):21-26.
[4] Su Yujie,Sun Jun,Rao Shengqi,et al.Photodynamic antimicrobial activity of hypocrellin A[J].J Photochem Photobiol B:Biol,2011,103(1):29-34.
[5] Xu Shangjie,Shen Chen,Zhang Manhua,et al.A novel method for the preparation of amino-substituted hypocrellin B[J].Bioorg Medl Chem Llett,2001,11(15):2045-2047.
[6] Xie Wenli,Wei Shaohua,Liu Jihua,et al.Combination anticancer therapy activity studies for the complex of hypocrellin A and gallium ion[J].Dyes and Pigments,2014,101:43-50.
[7] Su Yujie,Si Shaohong,Qiao Liwen,et al.The effect of a hypocrellin A enriched diet on egg yolk quality and hypocrellin A distributions in the meat of laying hens[J].Eur Food Res Technol,2011,232(6):935-940.
[8] Wu Houming,Lao Xiafei,Wang Qiwen,et al.The shiraiachromes:novel fungal perylenequinone pigments from Shiraia bambusicola[J].J Nat Prod,1989,52(5):948-951.
[9] Shen Xiaoye,Hu Yingju,Song Liang,et al.Improvement of hypocrellin production by a new fungal source and optimization of cultivation conditions[J].Biotechnol Biotech Eq,2016,30(4):819-826.
[10] Du Wen,Liang Zongqi,Zou Xiao,et al.Effects of microbial elicitor on production of hypocrellin by Shiraia bambusicola[J].Folia Microbiol,2013,58(4):283-289.
[11] Du Wen,Liang Jiandong,Han Yanfeng,et al.Nitric oxide mediates hypocrellin accumulation induced by fungal elicitor in submerged cultures of Shiraia bambusicola[J].Biotechnol Lett,2015,37(1):153-159.
[12] Cai Yujie,Liao Xiangru,Liang Xiaohui,et al.Induction of hypocrellin production by Triton X-100 under submerged fermentation with Shiraia sp. SUPER-H168[J].New Biotechnol,2011,28(6):588-592.
[13] Lei Xiuyun,Zhang Mingye,Ma Yanjun,et al.Transcriptomic responses involved in enhanced production of hypocrellin A by addition of Triton X-100 in submerged cultures of Shiraia bambusicola[J].J Ind Microbiol Biot,2017,44(10):1415-1429.
[14] Sun Chunxiao,Ma Yanjun,Wang Jianwen.Enhanced production of hypocrellin A by ultrasound stimulation in submerged cultures of Shiraia bambusicola[J].Ultrason Sonochem,2017,38:214-224.
[15] Yang Hailong,Xiao Caixia,Ma Wenxin,et al.The production of hypocrellin colorants by submerged cultivation of the medicinal fungus Shiraia bambusicola[J].Dyes and Pigments,2009,82(2):142-146.
[16] 项小燕,张中信,谢翎,等.竹黄菌液体培养下产竹红菌素的研究[J].广西植物,2012,32(2):264-268.
[17] 胡明明,蔡宇杰,廖祥儒,等.竹红菌素培养基的初步优化[J].食品与机械,2010,26(5):141-143.
[18] 史文玉,吕平,张同存.新型食品色素竹红菌素发酵培养基的研究[J].食品研究与开发,2016,37(6):182-185.
[19] Liu Bin,Bao Jianying,Zhang Zhibin,et al.Enhanced production of perylenequinones in the endophytic fungus Shiraia sp. Slf14 by calcium/calmodulin signal transduction[J].Appl Microbiol Biotechnol,2018,102(1):153-163.
[20] 彭思露,杨慧林,李尔汉,等.蛇足石杉内生真菌Shiraia sp.Slf14中Ⅲ型聚酮合酶的表达、纯化及生物信息学分析[J].江西师范大学学报:自然科学版,2015,39(4):430-434.
[21] Zhu Du,Wang Jixiang,Zeng Qinggui,et al.A novel endophytic Huperzine A:producing fungus,Shiraia sp. Slf14,isolated from Huperzia serrata[J].J Appl Microbiol,2010,109(4):1469-1478.
[22] Tong Zhiwu,Mao Lingwen,Liang Hailin,et al.Simultaneous determination of six perylenequinones in Shiraia sp. Slf14 by HPLC[J].J Liquid Chromatogr Relat Technol,2017,40(10):536-540.
[23] 颜日明,李希茜,汪涯,等.蛇足石杉内生真菌Shiraia sp. Slf14化学成分及其抑菌活性研究[J].天然产物研究与开发,2014,26(9):1393-1397.
[24] Yang Huilin,Wang Ya,Zhang Zhibin,et al.Whole-genome shotgun assembly and analysis of the genome of Shiraia sp. strain Slf14,a novel endophytic fungus producing huperzine A and hypocrellin A[J].Genome Announcements,2014,2(1):e00011-e00014.
[25] 韩钱松.竹黄菌Shiraia sp. SUPER-H168液态发酵产竹红菌素的优化[D].无锡:江南大学,2013.
[26] 高教琪,袁文杰,陈丽杰,等.通气量和菊粉浓度对克鲁维酵母乙醇发酵的影响[J].生物工程学报,2013,29(3):325-332.

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备注/Memo

备注/Memo:
收稿日期:2019-04-08基金项目:国家自然科学基金(31460021)和江西省自然科学基金(20151BAB204002)资助项目.通信作者:朱 笃(1971-),男,江西高安人,教授,博士,主要从事微生物资源及代谢调控研究.E-mail:zhudu12@163.com
更新日期/Last Update: 2019-10-10