[1]黄小兰,贾 伟,叶长盛,等.赣江下游河湖交错带微生物群落分布及其影响因素分析[J].江西师范大学学报(自然科学版),2019,(05):501-507.[doi:10.16357/j.cnki.issn1000-5862.2019.05.11]
　HUANG Xiaolan,JIA Wei,YE Changsheng,et al.The Analysis on Distribution of Microbial Community and Its Influencing Factors in the River-Lake Ecotone of Ganjiang River Downstream[J].Journal of Jiangxi Normal University:Natural Science Edition,2019,(05):501-507.[doi:10.16357/j.cnki.issn1000-5862.2019.05.11]
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赣江下游河湖交错带微生物群落分布及其影响因素分析()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年05期
页码:
501-507
栏目:
出版日期:
2019-10-10

文章信息/Info

Title:
The Analysis on Distribution of Microbial Community and Its Influencing Factors in the River-Lake Ecotone of Ganjiang River Downstream
文章编号:
1000-5862(2019)05-0501-07
作者:
黄小兰1; 2; 贾 伟2; 叶长盛1; 简正军2; 张 建3
1.东华理工大学地球科学学院,江西 南昌 330013; 2.江西师范大学地理与环境学院,江西 南昌 3300222; 3.浙江省义乌市上溪中学,浙江 义乌 322006
Author(s):
HUANG Xiaolan1; 2; JIA Wei2; YE Changsheng1; JIAN Zhengjun2; ZHANG Jian3
1.School of Geosciences,East China University of Technology,Nanchang Jiangxi 330013,China; 2.College of Geography and Environment,Jiangxi Normal University,Nanchang Jiangxi 330022,China; 3.Yiwu Shangxi Senior Middle School of Zhejiang province,Yiwu Zhejiang 322006,China
关键词:
赣江河湖交错带;  冬季;  夏季;  微生物;  影响因子
Keywords:
ecotone of the river-lake in Ganjiang River;  winter;  summer;  microorganism;  influence factors
分类号:
X 824
DOI:
10.16357/j.cnki.issn1000-5862.2019.05.11
文献标志码:
A
摘要:
目前关于细菌多样性及其与环境因子相互作用的研究大部分集中在滨海地区、海洋和湖泊,针对某一流域内冬季和夏季微生物分布差异的研究较少.通过选择赣江下游河湖交错带典型样点作采样分析,运用Miseq高通量测序技术分析手段,研究了赣江河湖交错带的典型水样点的群落分布特征.结果表明:赣江下游河湖交错带冬季共有6个优势微生物类群,分别是变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、蓝藻菌门(Cyanobacteria)和疣微菌门(Verrucomicrobia); 夏季共有变形菌门、拟杆菌门、放线菌门、蓝藻菌门、疣微菌门、绿弯菌门(Chloroflexi)和酸杆菌门(Acidobacteria)7个优势微生物类群.冬季和夏季最大的优势微生物类群都是变形菌门,其次为拟杆菌门.多样性指数表明夏季微生物群落丰富度和多样性高于冬季.采用Canoco软件做物种数量与环境因子之间的冗余分析,结果表明:影响冬季和夏季微生物分布特征的主要理化因子为总氮(TN)、总磷(TP)、pH值.不同微生物对水环境的变化具有一定的承受力.季节的变化引起的理化因子的改变对优势微生物类群分布影响较小.微生物群落分布特征受多重因子的共同影响.
Abstract:
At present,most studies on bacterial diversity and its interaction with environmental factors are concentrated in coastal areas,oceans and lakes,but there are few studies on the differences in microbial distribution in winter and summer in a certain basin.This paper analyzes the differences in the distribution of microbial communities in the summer and winter in the river-lake ecotone in the lower reaches of the Ganjiang River and its influencing factors.By selecting typical samples of the river-lake interlaced zone in the lower reaches of the Ganjiang River for sampling analysis,the Mesq high-throughput sequencing technique is used to study the community distribution characteristics of typical water samples in the interlaced zone of the Ganjiang River.The results show that there are 6 dominant microbial groups in the river-lake interlaced zone in the lower reaches of the Ganjiang River in winter,namely Proteobacteria,Bacteroides,Thick-walled bacteria,Actinomycetes,Cyanobacteria and Sputum micro-bacteria.There are 7 dominant microbial groups of bacteria,Bacteroidetes,actinomycetes,cyanobacteria,sputum microphylaxis,green bacillus and acid bacillus.The most dominant microbial group in winter and summer is Proteobacteria,followed by Bacteroides.The diversity index indicates that the abundance and diversity of microbial communities in summer are higher than those in winter.Physical and chemical factors analysis show that the heavy metal content in summer is lower than that in winter,but there is still a certain degree of heavy metal pollution of cadmium,lead and mercury in the lower reaches of Gannjiang River,and the eutrophication of water is more serious.The Canoco software is used to analyze the redundancy between species quantity and environmental factors.The results show that the main physical and chemical factors affecting the distribution characteristics of microbes in winter and summer are total nitrogen(TN),total phosphorus(TP)and pH value.Different microorganisms have a certain tolerance to changes in the water environment.The changes in physical and chemical factors caused by seasonal changes have little effect on the distribution of dominant microbial groups.The distribution characteristics of microbial communities are not only affected by single factor changes but also by multiple factors.

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备注/Memo

备注/Memo:
收稿日期:2019-01-02基金项目:国家自然科学基金(41561002)资助项目.作者简介:黄小兰(1968-),女,江西赣州市人,副研究员,博士,主要从事城市化水环境研究.E-mail:huangxiaolanlan@163.com
更新日期/Last Update: 2019-10-10