[1]过七根,熊粟栗,肖 瑶,等.铜绿微囊藻附生细菌多样性及其溶磷活性研究[J].江西师范大学学报(自然科学版),2020,(01):76-81.[doi:10.16357/j.cnki.issn1000-5862.2020.01.13]
 GUO Qigen,XIONG Suli,XIAO Yao,et al.The Diversity and Phosphate Solubilization Activity of Microcystis aeruginosa-Associated Bacteria[J].Journal of Jiangxi Normal University:Natural Science Edition,2020,(01):76-81.[doi:10.16357/j.cnki.issn1000-5862.2020.01.13]
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铜绿微囊藻附生细菌多样性及其溶磷活性研究()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年01期
页码:
76-81
栏目:
化学与生命科学
出版日期:
2020-02-10

文章信息/Info

Title:
The Diversity and Phosphate Solubilization Activity of Microcystis aeruginosa-Associated Bacteria
文章编号:
1000-5862(2020)01-0076-06
作者:
过七根熊粟栗肖 瑶徐常龙张炳火*
九江学院药学与生命科学学院,江西 九江 332000
Author(s):
GUO QigenXIONG SuliXIAO YaoXU ChanglongZHANG Binghuo*
College of Pharmacy and Life Sciences,Jiujiang University,Jiujiang Jiangxi 332000,China
关键词:
铜绿微囊藻 附生细菌 溶磷活性 磷酸酶
Keywords:
Microcystis aeruginosa associated bacteria phosphate solubilization activity phosphatase
分类号:
S 154.2; X 172
DOI:
10.16357/j.cnki.issn1000-5862.2020.01.13
文献标志码:
A
摘要:
为了解铜绿微囊藻附生细菌的多样性及其溶磷活性,采用平板稀释涂布法分离细菌,并利用16S rRNA基因序列分析确定其分类学地位,用钼锑抗比色法检测其溶磷活性,用苯磷酸二钠法检测其磷酸酶活性.结果表明:分离的细菌分属于4个门,即放线菌门、变形菌门、拟杆菌门和异常球菌-栖热菌门; 其中83%以上的细菌至少对卵磷脂、植酸钙和磷酸钙中的1种具有溶磷活性,94%以上的细菌表现出1~3种磷酸酶活性,且导致磷培养基的pH值下降.铜绿微囊藻附生细菌中存在丰富的溶磷微生物,这些微生物对藻类获取足够的可溶性磷元素可能具有重要的
Abstract:
In order to understand the diversity and phosphate solubilization activity of Microcystis aeruginosa-associated bacteria,dilution plating procedure is used to isolate the bacteria,and the 16S rRNA gene sequences are used to determine the taxonomic status of the bacteria,the molybdenum antimony resistance colorimetric and disodium phenyl phosphate methods are used to analyze solubilizing-phosphate activities and phosphatase activities of the bacteria,respectively.The results show that the isolates belong to four phylums,i.e.Actinobacteria,Proteobacteria,Bacteroidetes and Deinococcus-Thermus,respectively.And more than 83% of these bacteria exhibit solubilizing-phoshate activities at least on one of the three phosphorus sources such as lecithin,phytin and tricalcium phosphate,and more than 94% of these bacteria exhibit 1~3 kinds of phosphatases,and result in decline of the pH value of phosphorus media.There are abundant solubilizing-phosphate bacteria in the Microcystis aeruginosa-associated bacteria,and these microorganisms probably play an important role for the algae to obtain enough soluble phosphorus.

参考文献/References:

[1] 赵洋,叶兴林,杨琴,等.2(4-氯苯基)-4(4-甲氧基苯基)喹唑啉抑制微囊藻生长的研究及应用前景探究[J].江西师范大学学报:自然科学版,2014,38(3):314-317.
[2] Gumbo J R,Cloete T E,Van Zyl G J,et al.The viability assessment of Microcystis aeruginosa cells after co-culturing with Bacillus mycoides B16 using flowcytometry[J].Physics and Chemistry of the Earth,2014,72/73/74/75:24-33.
[3] 李汉全,张炳火,杨建远,等.Streptomyces eurocidicus JXJ 0089对铜绿微囊藻的抑制[J].江苏农业学报,2015,31(5):1037-1044.
[4] Zhang Binghuo,Chen Wei,Li Haiquan,et al.An antialgal compound produced by Streptomyces jiujiangensis JXJ 0074T[J].Applied Microbiology and Biotechnology,2015,99(18):7673-7683.
[5] 中华人民共和国农业部种植业管理司.NY/T 1847-2010.微生物肥料生产菌株质量评价通用技术要求[S].北京:中国农业出版社,2010.
[6] 徐丽华,李文均,刘志恒,等.放线菌系统学:原理、方法及实践[M].北京:科学出版社,2007.
[7] Nautiyal C S.An efficient microbiological growth medium for screening phosphate solubilizing microorganisms[J].FEMS Microbiology Letters,1999,170(1):265-270.
[8] 赵兰坡,姜岩.土壤磷酸酶活性测定方法的探讨[J].土壤通报,1986,3:138-141.
[9] 陈丹阳,李汉全,张炳火,等.2株解磷细菌的解磷活性及作用机制研究[J].中国生态农业学报,2017,25(3):410-418.
[10] Shi Limei,Cai Yuanfeng,Yang Hualin,et al.Phylogenetic diversity and specificity of bacteria associated with Microcystis aeruginosa and other cyanobacteria[J].Joumal of Environmental Science,2009,21(11):1581-1590.
[11] 李汉全,张炳火,杨建远,等.Streptomyces eurocidicus JXJ 0089对铜绿微囊藻的抑制[J].江苏农业学报,2015,31(5):1037-1044.
[12] 邹迪,肖琳,杨柳燕,等.不同形态磷源对铜绿微囊藻与附生假单胞菌磷代谢的影响[J].环境科学,2005,26(3):118-121.
[13] 赵婕,李建宏,管章玲,等.1株产碱性磷酸酶附生菌对微囊藻生长的影响[J].湖泊科学,2011,23(1):49-55.
[14] Zhao Guiying,Du Jingjing,Yong Jia,et al.The importance of bacteria in promoting algal growth in eutrophic lakes with limited available phosphorus[J].Ecological Engineering,2012,42(9):107-111.
[15] Chen Yepei,Pekha P D,Arun A B,et al.Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities[J].Applied Soil Ecology,2006,34(1):33-41.
[16] Hameeda B,Reddy Y H,Rupela O P,et al.Effect of carbon substrates on rock phosphate solubilization by bacteria from composts and macrofauna[J].Current Microbiology,2006,53(4):298-302.
[17] 张英,芦光新,谢永丽,等.溶磷菌分泌有机酸与溶磷能力相关性研究[J].草地学报,2015,23(5):1033-1038.

备注/Memo

备注/Memo:
收稿日期:2018-12-16
基金项目:国家自然科学基金(31060010),江西省科技厅课题(20111BBG70012-4),江西省环保厅科技课题(JXHBKJ2013-14),江西省教育厅科技课题(GJJ151080)和九江学院重点课题(201511)资助项目.
作者简介:过七根(1978-),男,江西抚州人,副教授,主要从事微生物学相关研究.E-mail:936452840@qq.com
通信作者:张炳火(1968-),男,江西九江人,教授,博士,主要从事蓝藻水华防治研究
更新日期/Last Update: 2020-02-10