[1]蔡险峰,谢 振,资鹏鹏,等.三七总皂苷壳聚糖纳米粒冻干粉的制备工艺研究[J].江西师范大学学报(自然科学版),2020,(02):190-195.[doi:10.16357/j.cnki.issn1000-5862.2020.02.14]
　CAI Xianfeng,XIE Zhen,ZI Pengpeng,et al.The Study on Preparation Technology of Lyophilized Powder of Panax Notoginseng Saponin Chitosan Nanoparticles[J].Journal of Jiangxi Normal University:Natural Science Edition,2020,(02):190-195.[doi:10.16357/j.cnki.issn1000-5862.2020.02.14]
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三七总皂苷壳聚糖纳米粒冻干粉的制备工艺研究()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年02期
页码:
190-195
栏目:
化学与生命科学
出版日期:
2020-04-10

文章信息/Info

Title:
The Study on Preparation Technology of Lyophilized Powder of Panax Notoginseng Saponin Chitosan Nanoparticles
文章编号:
1000-5862(2020)02-0190-06
作者:
蔡险峰; 谢 振; 资鹏鹏; 徐贤柱; 游清徽; 王曼莹*
江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西 南昌 330022
Author(s):
CAI Xianfeng; XIE Zhen; ZI Pengpeng; XU Xianzhu; YOU Qinghui; WANG Manying*
College of Life Science,Key Laboratory of the Conservation and Sustainable Utilization for Subtropical Plant Resources of Jiangxi Province,Jiangxi Normal University,Nanchang Jiangxi 330022,China
关键词:
三七总皂苷;  壳聚糖;  冻干保护剂;  冻干粉
Keywords:
panax notoginseng saponins;  chitosan;  lyoprotectant;  lyophilized powder
分类号:
R 283.1
DOI:
10.16357/j.cnki.issn1000-5862.2020.02.14
文献标志码:
A
摘要:
为提高三七总皂苷壳聚糖纳米粒(PNS-NPs)的稳定性,采用冷冻干燥法制备冻干粉并优化其冻干工艺.通过离子凝胶法制备PNS-NPs,以再分散性、粒径分布及微观形态及药物渗漏率为指标,进行全面实验和配伍实验筛选最优冻干工艺.结果表明: PNS-NPs冻干粉最佳制备工艺为预冻时间12 h、冻干保护剂为2.5%蔗糖+2.5%海藻糖.在该条件下制得的冻干粉分散性好、无粘连,扫描电镜显示其微观形貌呈球形; 分散后粒径为(138.30±3.15)nm,相比于冻干前原液有所增加,但各组分药物渗漏率均未超过5%.PNS-NPs冻干粉有望成为PNS纳米新剂型.
Abstract:
To improve the stability of panax notoginseng saponin chitosan nanoparticles(PNS-NPs),freeze-dried powder is prepared by freeze-drying method and its freeze-drying process is optimized.PNS-NPs are prepared by ion cross-linking method.The optimal lyophilization process is performed by comprehensive experiments and compatibility experiments with re-dispersibility,particle size distribution,microscopic morphology and drug leakage rate.The results show that the optimal preparation process of PNS-NPs lyophilized powder is 12 h pre-freezing time and 2.5% sucrose+2.5% trehalose mixed protective agent.The lyophilized powder prepared by this condition has good dispersibility and no adhesion.Scanning electron microscopy result shows that the microscopic morphology is spherical.The particle size after dispersion is(138.30±3.15)nm,which is increased compared with the original solution before lyophilization,but the drug leakage rates don't exceed 5%.PNS-NPs freeze-dried powder is expected to become a new nano-drug formulation of PNS.

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备注/Memo

备注/Memo:
收稿日期:2019-10-16
基金项目:国家十三五专项(2018ZX09721002-008),江西省亚热带植物资源保护与利用重点实验室开放基金(YRD201911)和江西省普通本科高校中青年教师发展计划访问学者专项资金资助项目.
作者简介:蔡险峰(1968-),男,浙江温州人,副教授,主要从事壳聚糖药用辅料的开发与利用研究.E-mail:jxcxf68@126.com
通信作者:王曼莹(1944-),女,江西南昌人,教授,博士,主要从事食品工业用酶制剂研究与应用.E-mail:
更新日期/Last Update: 2020-04-10