[1]刘中华,于慧瑛,伍芳艺,等.1株盐湖噬菌体CJL-7的分离及其生物学特性研究[J].江西师范大学学报(自然科学版),2021,(03):241-245.[doi:10.16357/j.cnki.issn1000-5862.2021.03.04]
 LIU Zhonghua,YU Huiying,WU Fangyi,et al.The Isolation and Biological Characteristics of a Bacteriophage CJL-7 from Salt Lake[J].Journal of Jiangxi Normal University:Natural Science Edition,2021,(03):241-245.[doi:10.16357/j.cnki.issn1000-5862.2021.03.04]
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1株盐湖噬菌体CJL-7的分离及其生物学特性研究()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年03期
页码:
241-245
栏目:
生命科学
出版日期:
2021-06-10

文章信息/Info

Title:
The Isolation and Biological Characteristics of a Bacteriophage CJL-7 from Salt Lake
文章编号:
1000-5862(2021)03-0241-05
作者:
刘中华于慧瑛伍芳艺曹建斌李 新*
运城学院生命科学系,山西 运城 044000
Author(s):
LIU ZhonghuaYU HuiyingWU FangyiCAO JianbinLI Xin*
Department of Life Sciences,Yuncheng University,Yuncheng Shanxi 044000,China
关键词:
盐湖 噬菌体 嗜盐菌 生物学特性
Keywords:
salt lake bacteriophage halophilic bacteria biological characteristics
分类号:
X 172
DOI:
10.16357/j.cnki.issn1000-5862.2021.03.04
文献标志码:
A
摘要:
以嗜盐菌为宿主,从山西省运城盐湖湖水中分离出1株噬菌体,经浓缩纯化后用电镜观察其形态,并进行限制性酶切、蛋白组成分析及生物学特性研究.研究结果表明:分离获得的CJL-7为双链DNA烈性噬菌体,属微小噬菌体科; 其头部为正多面体结构,直径约为80 nm,在其衣壳中至少含3种主要结构蛋白; 该噬菌体含有脂质包膜,宿主范围较广; 低盐处理对该噬菌体的效价几乎没有影响,其活性在30~50 ℃范围内较稳定,在40 ℃时效价最高,在碱性条件下活性更强.
Abstract:
There are abundant bacteriophages in salt lakes.Isolation and preliminary identification of their biological characteristics will be helpful for further development and utilization.Using halophilic bacteria as the host bacteria,a bacteriophage is isolated from Yuncheng salt lake and named CJL-7 in this study.Then it is observed under electron microscopy after concentration and purification.Finally,restriction enzyme digestion,protein composition analysis and biological characteristics are studied.The results show that CJL-7 is a double stranded DNA virulent phage and belongs to the microphage family.A polyhedron structure is in its head with a diameter of about 80 nm.There are at least three major structural proteins in its capsid.The bacteriophage contains lipid-encapsulated membranes and has a wide host range.Low salt treatment has little effect on its titer.The bacteriophage activity is stable in the range of 30—50 ℃ and has the highest titer at 40 ℃.The activity is enhanced under alkaline conditions.

参考文献/References:

[1] 郑绵平,齐文.我国盐湖资源及其开发利用[J].矿产保护与利用,2006,50(5):45-50.
[2] 龙启福,朱德锐,韩睿,等.嗜盐菌相溶物质合成与转运调节机制[J].环境科学与技术,2011,34(9):63-66.
[3] Oren A.Halophilic microbial communities and their environments[J].Current Opinion in Biotechnology,2015,33(6):119-124.
[4] Morozkina E V,Slutskaia E S,Fedorova T V,et al.Extremophilic microorganisms:biochemical adaptation and biotechnological application[J].Applied Biochemistry and Microbiology,2010,46(1):1-14.
[5] Li Xin,Yu Huiying.Biodiversity and screening of halophilic bacteria with hydrolytic and antimicrobial activities from Yuncheng salt lake,China[J].Biologia,2015,70(2):151-156.
[6] Hendrix R W.Bacteriophage genomics[J].Current Opinion in Microbiology,2003,6(5):506-511.
[7] Kurzpa A,Dbrowska K,S'witaa-Jeleń K,et al.Molecular modification of T4 bacteriophage proteins and its potential application:review[J].Folia Microbiologica,2009,54(1):5-15.
[8] Atanasova N S,Bamford D H,Oksanen H M.Virus-host interplay in high salt environments[J].Environmental Microbiology Reports,2016,8(4):431-444.
[9] Calvo C,de La Paz A G,Bejar V,et al.Isolation and characterization of phage F9-11 from a lysogenic Deleya halophila strain[J].Current Microbiology,1988,17(1):49-53.
[10] Atanasova N S,Oksanen H M,Bamford D H.Haloviruses of archaea,bacteria,and eukaryotes[J].Current Opinion in Microbiology,2015,25(6):40-48.
[11] 萨姆布鲁克 J,格林 M R.分子克隆实验指南[M].4版.北京:科学出版社,2017:32.
[12] Narasingarao P,Podell S,Ugalde J A,et al.De novo metagenomic assembly reveals abundant novel major lineage of Archaea in hypersaline microbial communities[J].ISME Journal,2012,6(1):81-93.
[13] 傅超群,白萌,王永霞,等.高盐环境可培养细菌噬菌体的研究进展[J].微生物学通报,2017,44(4):920-928.
[14] Aalto A P,Bitto D,Ravantti J J,et al.Snapshot of virus evolution in hypersaline environments from the characterization of a membrane-containing Salisaeta icosahedral phage 1[J].Proc Natl Acad Sci USA,2012,109(18):7079-7084.
[15] Wang Chuanxu,Li Xin.JMT-1:a novel,spherical lytic halotolerant phage isolated from Yuncheng saline lake[J].Brazilian Journal of Microbiology,2018,49(1):262-268.
[16] Andrew M Q K,Elliot L,Michael J,et al.Virus taxonomy:ninth report of the international committee on taxonomy of viruses[R].San Diego:Elsevier Academic Press,2012:385-386.
[17] Vreeland R H.Advances in understanding the biology of halophilic microorganisms[M].Berlin:Springer Press,2012:81-116.
[18] Rothschild L J,Mancinelli R L.Life in extreme environments[J].Nature,2001,409(6823):1092-1101.
[19] Kukkaro P,Bamford D H.Virus-host interactions in environments with a wide range of ionic strengths[J].Environmental Microbiology Reports,2009,1(1):71-77.
[20] Porter K,Tang S L,Chen C P,et al.PH1:an archaeovirus of Haloarcula hispanica related to SH1 and HHIV-2[J].Archaea,2013,2013(3):456318.
[21] Hardies S C,Hwang Y J,Hwang C Y,et al.Morphology, physiological characteristics,and complete sequence of marine bacteriophage RIO-1 infecting Pseudoalteromonas marina[J].Journal of Virology,2013,87(16):9189-9198.
[22] Roine E,Kukkaro P,Paulin L,et al.New,closely related haloarchaeal viral elements with different nucleic acid types[J].Journal of Virology,2010,84(7):3682-3689.

备注/Memo

备注/Memo:
收稿日期:2020-12-04
基金项目:国家自然科学青年基金(31300002),山西省重点学科建设(FSKSC,2018),山西省高等学校优秀青年学术带头人支持计划(2016)和山西省应用基础研究(201701D121088)资助项目.
通信作者:李 新(1981—),山东莱阳人,教授,博士,主要从事微生物资源开发与应用研究.E-mial:lixin-eva@163.com
更新日期/Last Update: 2021-06-10