[1]宋 玮,钱群丽,卢阳阳,等.基于荧光纳米传感的DEHP快速检测方法研究[J].江西师范大学学报(自然科学版),2021,(04):432-437.[doi:10.16357/j.cnki.issn1000-5862.2021.04.18]
 SONG Wei,QIAN Qunli,LU Yangyang,et al.The Fluorescent Nanosensor-Based Rapid Detection Assay for DEHP[J].Journal of Jiangxi Normal University:Natural Science Edition,2021,(04):432-437.[doi:10.16357/j.cnki.issn1000-5862.2021.04.18]
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基于荧光纳米传感的DEHP快速检测方法研究()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年04期
页码:
432-437
栏目:
化学
出版日期:
2021-08-10

文章信息/Info

Title:
The Fluorescent Nanosensor-Based Rapid Detection Assay for DEHP
文章编号:
1000-5862(2021)04-0432-06
作者:
宋 玮钱群丽卢阳阳宋卫国*
上海市农业科学院,上海 201403
Author(s):
SONG WeiQIAN QunliLU YangyangSONG Weiguo*
Shanghai Academy of Agricultural Sciences,Shanghai 201403,China
关键词:
邻苯二甲酸二(2-乙基)己酯 快速检测 荧光纳米传感器
Keywords:
DEHP rapid detection assay fluorescent nanosensor
分类号:
O 657
DOI:
10.16357/j.cnki.issn1000-5862.2021.04.18
文献标志码:
A
摘要:
采用一锅法制备了超支化聚乙烯亚胺-铜纳米簇(hPEI-Cu NCs),并利用其构建“turn on”荧光纳米传感器对邻苯二甲酸二(2-乙基)己酯(DEHP)进行定量快速检测.hPEI-Cu NCs的最佳激发波长为385 nm,在510 nm处有强发射,透射电镜(TEM)分析结果表明:其形态呈现良好的分散性,粒径为(1.3±0.3)nm.在加入Cu2+后,hPEI-Cu NCs的荧光被Cu2+淬灭; 但当存在DEHP时,Cu2+不能淬灭hPEI-Cu NCs的荧光,且使其荧光强度比不加DEHP时有所上升.随着DEHP质量浓度的增加,hPEI-Cu NCs的荧光逐渐增强,从而达到检测DEHP的目的.荧光强度变化率((I-I0)/I0)与DEHP的质量分数在1.0~7.0 mg·kg-1的范围内呈现良好的线性关系,当信噪比为3 时计算出DEHP的最低检出限约为0.4 mg·kg-1.
Abstract:
A "turn on" fluorescent nanosensor is constructed for rapid detection of DEHP by using one-pot method prepared hPEI-Cu NCs.The best excitation wavelength of hPEI-Cu NCs is 385 nm,and has a strong emission at 510 nm.The morphology of hPEI-Cu NCs is characterized by transmission electron microscopy(TEM).It shows good dispersion and its particle size is(1.3±0.3)nm.After adding Cu2+,the fluorescence of hPEI-Cu NCs is quenched by Cu2+.When DEHP is present,the fluorescence will rise becasuse the fluorescence of hPEI-Cu NCs can not be quenched by Cu2+.With the increase of DEHP concentration,the fluorescence of hPEI-Cu NCs gradually increased,so as to achieve the purpose of detecting DEHP.The fluorescence intensity change rate and DEHP concentration show a good linear relationship in the range of 1.0—7.0 mg·kg-1.When the signaql-to-noise ratio is 3,the lowest detection limit is calculated to be about 0.4 mg·kg-1.

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备注/Memo

备注/Memo:
收稿日期:2021-03-25
基金项目:国家自然科学基金面上课题(31772189)和上海市科委青年科技英才扬帆计划(18YF1421000)资助项目.
作者简介:宋 玮(1989—),女,宁夏吴忠人,副研究员,博士,主要从事农产品质量安全监测及快速检测技术研究.E-mail:songwei890214@163.com
通信作者:宋卫国(1979—),男,山东成武人,研究员,博士,主要从事农产品质量安全检测及农药毒理学研究.E-mail:sunvegous@hotmail.com
更新日期/Last Update: 2021-08-10