[1]丰 磊,魏伟群*,付鸣佳*,等.枯草芽孢杆菌SX3411产抑菌物质的发酵条件优化[J].江西师范大学学报(自然科学版),2021,(05):503-513.[doi:10.16357/j.cnki.issn1000-5862.2021.05.09]
　FENG Lei,WEI Weiqun*,FU Mingjia*,et al.The Optimization of Fermentation Conditions Producing Antimicrobial Substances from Bacillus subtilis SX3411[J].Journal of Jiangxi Normal University:Natural Science Edition,2021,(05):503-513.[doi:10.16357/j.cnki.issn1000-5862.2021.05.09]
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枯草芽孢杆菌SX3411产抑菌物质的发酵条件优化()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年05期
页码:
503-513
栏目:
出版日期:
2021-10-10

文章信息/Info

Title:
The Optimization of Fermentation Conditions Producing Antimicrobial Substances from Bacillus subtilis SX3411
文章编号:
1000-5862(2021)05-0503-11
作者:
丰 磊1; 魏伟群2*; 付鸣佳1*; 肖世平2; 叶德晓1; 钟雪晴1; 黄紫妍1
1.江西师范大学生命科学学院,江西 南昌 330022; 2.江西天佳生物工程股份有限公司,江西 南昌 330200
Author(s):
FENG Lei1; WEI Weiqun2*; FU Mingjia1*; XIAO Shiping2; YE Dexiao1; ZHONG Xueqing1; HUANG Ziyan1
1.College of Life Sciences,Jiangxi Normal University,Nanchang Jiangxi 330022,China; 2.Jiangxi Tianjia Bioengineering Company Limited,Nanchang Jiangxi 330200,China
关键词:
枯草芽孢杆菌SX3411菌株;  抑菌物质;  发酵;  单因素分析;  响应面法
Keywords:
Bacillus subtilis SX3411 strain;  bacteriostatic substances;  fermentation;  single-factor analysis;  response surface methodology
分类号:
Q 815
DOI:
10.16357/j.cnki.issn1000-5862.2021.05.09
文献标志码:
A
摘要:
枯草芽孢杆菌SX3411菌株自身带有subtilomycin基因簇,其主要的抑菌成分为subtilomycin.该文采用单因素分析法分析了枯草芽孢杆菌SX3411在不同发酵条件和营养成分时对抑菌物质产生的影响.根据单因素分析,对枯草芽孢杆菌SX3411产抑菌物质设计了响应面法分析,通过响应面法获得了最优的发酵条件和最佳营养成分配比.结果表明:最佳培养条件是初始pH值为8.25、转速为169 r?min-1、温度为30.24 ℃、装液量为37.9%、接种量为1.46%,抑菌圈直径预测值为30.04 mm; 最佳培养基的成分为葡萄糖11.4 g?L-1、酵母粉5.0 g?L-1、ZnSO4 0.000 56 g?L-1、CaCl2 0.059 5 g?L-1,抑菌圈直径预测值为27.83 mm.实际检测值与预测值完全吻合.实验结果为生产羊毛硫细菌素subtilomycin提供了依据.
Abstract:
Bacillus subtilis SX3411 strain has subtilomycin gene cluster,and its main antimicrobial component is subtilomycin.The effects of Bacillus subtilis SX3411 on the production of antimicrobial substances under different fermentation conditions and nutritional components are analyzed by single factor method.According to the single factor analysis,the response surface methodology is designed to analyze the bacteriostatic substances produced by Bacillus subtilis SX3411.The optimal fermentation conditions and the optimal nutrient composition are obtained by response surface methodology.The results show that the optimum culture conditions are initial pH 8.25,rotational speed 169 r?min-1,temperature 30.24 ℃,liquid loading 37.9%,inoculation quantity 1.46%,and the predicted value of bacteriostasis circle diameter is 30.04 mm.The optimum medium is glucose 11.4 g?L-1,yeast powder 5.0 g?L-1,zinc sulfate 0.000 56 g?L-1,calcium chloride 0.059 5 g?L-1,and the predicted value of bacteriostasis circle diameter is 27.83 mm.The actual measured value is in good agreement with the predicted value.This provides a basis for the production of lantibiotic subtilomycin.

参考文献/References:

[1] Chatterjee C,Paul M,Xie Lili,et al.Biosynthesis and mode of action of lantibiotics[J].Chem Rev,2005,105(2):633-684.
[2] Knerr P J,Van D.Discovery,biosynthesis,and engineering of lantipeptides[J].Annual Review of Biochemistry,2012,81(1):479-505.
[3] Müller W M,Schmiederer T,Ensle P,et al.In vitro biosynthesis of the prepeptide of type-Ⅲ lantibiotic labyrinthopeptin A2 including formation of a C—C bond as a post-translational modification[J].Angew Chem Int Ed,2010,49(13):2436-2440.
[4] Pag U,Sahl H G.Multiple activities in lantibiotics-models for the design of novel antibiotics[J].Curr PHarm Des,2002,8(9):815-833.
[5] Goto Y,Li Bo,Claesen J,et al.Discovery of unique lanthionine synthetases reveals new mechanistic and evolutionary insights[J].PLoS Biol,2010,8(3):e1000339.
[6] Kodani S,Hudson M E,Durrant M C,et al.The SapB morpHogen is a lantibiotic-like peptide derived from the product of the developmental gene ramS in Streptomyces coelicolor[J].Proc Natl Acad Sci USA,2004,101(31):11448-11453.
[7] Piper C,Cotter P D,Ross R P,et al.Discovery of medically significant lantibiotics[J].Current Drug Discovery Technologies,2009,6(1):1-18.
[8] Breukink E,Wiedemann I,Van K C,et al.Use of the cell wall precursor lipid II by a pore-forming peptide antibiotic[J].Science,1999,286(5448):2361-2364.
[9] Gross E,Morell J L.Structure of nisin[J].Journal of the American Chemical Society,1971,93(18):4634-4635.
[10] Rogers L A.The inhibiting effect of Streptococcus lactis on Lactobacillus bulgaricus[J].Journal of Bacteriology,1928,16(5):321-325.
[11] Delves-Broughton J,Blackburn P,Evans R J,et al.Applications of the bacteriocin,nisin[J].Antonie van Leeuwenhoek,1996,69(2):193-202.
[12] Phelan R W,Barret M,Cotter P D,et al.Subtilomycin:a New Lantibiotic from Bacillus subtilis Strain MMA7 Isolated from the Marine Sponge Haliclona simulans[J].Mar Drugs,2013,11(6):1878-1898.
[13] Deng Yun,Li Congzi,Zhu Yiguang,et al.ApnI,a transmembrane protein responsible for subtilomycin immunity,unveils a novel model for lantibiotic immunity[J].Applied and Environmental Microbiology,2014,80(20):6303-6315.
[14] 李晓然,叶德晓,付鸣佳,等.枯草芽孢杆菌SX3411 产羊毛硫细菌素subtilomycin 的初步鉴定与理化特性分析[J].食品与发酵工业,2019,45(12):46-54.

备注/Memo

备注/Memo:
收稿日期:2021-03-30
基金项目:国家自然科学基金(31760601)和南昌市重大科技攻关课题(2020)资助项目.
通信作者:付鸣佳(1964—),男,江西高安人,教授,博士,主要从事药用真菌和益生菌研究.E-mail:mingjiafu@126.com
魏群伟(1984—),男,江西南昌人,工程师,主要从事发酵工程研究.E-mail:qunweiwei@163.com
更新日期/Last Update: 2021-10-10