[1]张 帆,袁 媛,曾 艳,等.赤红球菌甲苯胁迫响应蛋白基因的克隆、生物信息学分析和表达变化研究[J].江西师范大学学报(自然科学版),2022,(02):140-146.[doi:10.16357/j.cnki.issn1000-5862.2022.02.05]
　ZHANG Fan,YUAN Yuan,ZENG Yan,et al.The Cloning,Bioinformatic Analysis and Expression Variation of a Gene Cloning Toluene Stress Response Protein in Rhodoccocus ruber[J].Journal of Jiangxi Normal University:Natural Science Edition,2022,(02):140-146.[doi:10.16357/j.cnki.issn1000-5862.2022.02.05]
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赤红球菌甲苯胁迫响应蛋白基因的克隆、生物信息学分析和表达变化研究()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2022年02期
页码:
140-146
栏目:
生命科学
出版日期:
2022-03-25

文章信息/Info

Title:
The Cloning,Bioinformatic Analysis and Expression Variation of a Gene Cloning Toluene Stress Response Protein in Rhodoccocus ruber
文章编号:
1000-5862(2022)02-0140-07
作者:
张 帆; 袁 媛; 曾 艳; 付 薇; 彭 仁*
江西师范大学生命科学学院,江西 南昌 330022
Author(s):
ZHANG Fan; YUAN Yuan; ZENG Yan; FU Wei; PENG Ren*
College of Life Science,Jiangxi Normal University,Nanchang Jiangxi 330022,China
关键词:
赤红球菌;  有机溶剂胁迫;  荧光定量PCR;  生物信息学
Keywords:
Rhodoccocus ruber;  orgainc solvents stress;  fluorescence quantitative PCR;  bioinformatics
分类号:
Q 89
DOI:
10.16357/j.cnki.issn1000-5862.2022.02.05
文献标志码:
A
摘要:
从赤红球菌SD3中克隆了一个甲苯胁迫响应蛋白基因tsrp1,该基因长度为417 bp,在GenBank的登录号为MK371001.该基因编码的蛋白质Tsrp1由138个氨基酸残基组成,理论分子量为14.2 kDa,理论等电点为4.82,不稳定系数为18.68,总平均疏水指数为-0.154,属于酸性稳定的亲水蛋白.Tsrp1无信号肽和二硫键、无跨膜区、无保守结构域,存在于细胞质中.在R.ruber SD3中Tsrp1蛋白与R. rhodochrous、R. zopfii、R. pyridinivorans、R. hoagii、M. brevis和C. manganitolerans中的序列相似蛋白的相似性分别为94.93%、71.29%、67.96%、44.58%、42.05% 和35.00%.通过分子对接发现Tsrp1与c-di-GMP存在相互作用.在甲苯和苯酚胁迫下,在赤红球菌SD3中tsrp1基因的表达分别为原来的40.19倍和14.73倍.
Abstract:
Agene of tsrp1 encoding toluene stress response protein is cloned from Rhodoccocus ruber SD3.The length of the gene is 417 bp with the accession number of MK371001 in GenBank.It encodes a protein of 138 amino acid residues with an estimated molecular weight of 14. 2 kD and an isoelectric point of 4.82.It also has a instability index of 18.68 and a grand average of hydropathicity of -0.154,and thus it is acidic,stable and hydrophilic protein.The protein has no signal peptide,disulfide bonds and conservative domain.It exists in the cytoplasm.Tsrp1 in Rhodoccocus ruber SD3 has a similarity of 94.93%,71.29%,67.96%,44.58%,42.05% and 35.00% with sequence similar proteins in R. rhodochrous,R. zopfii,R. pyridinivorans,R. hoagii, M. brevis and C. manganitolerans,respectively.Tsrp1 is found to interact with c-di-GMP by molecular docking.The expression of tsrp1 gene has an increase of 40.19 folds and 14.73 folds under toluene and phenol stress,respectively.

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备注/Memo

备注/Memo:
收稿日期:2021-10-18
基金项目:国家自然科学基金(31960011)和江西省研究生创新专项资金(YC2020-S185)资助项目.
通信作者:彭 仁(1972—),男,江西丰城人,教授,博士,主要从事微生物生物化学研究.E-mail:renpeng@jxnu.edu.cn
更新日期/Last Update: 2022-03-25