[1]吴贵贵,韩 萍,钟素珍,等.Streptomyces albovinaceus DSM 40136中环二肽的盐胁迫发掘[J].江西师范大学学报(自然科学版),2022,(03):247-250.[doi:10.16357/j.cnki.issn1000-5862.2022.03.05]
　WU Guigui,HAN Ping,ZHONG Suzhen,et al.The Mining of the Cyclodipeptide from Streptomyces albovinaceus DSM 40136 Through Salt Stress[J].Journal of Jiangxi Normal University:Natural Science Edition,2022,(03):247-250.[doi:10.16357/j.cnki.issn1000-5862.2022.03.05]
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Streptomyces albovinaceus DSM 40136中环二肽的盐胁迫发掘()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2022年03期
页码:
247-250
栏目:
生命与环境科学
出版日期:
2022-05-25

文章信息/Info

Title:
The Mining of the Cyclodipeptide from Streptomyces albovinaceus DSM 40136 Through Salt Stress
文章编号:
1000-5862(2022)03-0247-04
作者:
吴贵贵1; 韩 萍1; 钟素珍1; 谢运昌1*; 朱 笃1; 2*
1.江西师范大学生命科学学院,江西 南昌 330022; 2.江西科技师范大学生命科学学院,江西省生物加工过程重点实验室,江西 南昌 330038
Author(s):
WU Guigui1; HAN Ping1; ZHONG Suzhen1; XIE Yunchang1*; ZHU Du1; 2*
1.College of Life Sciences,Jiangxi Normal University,Nanchang Jiangxi 330022,China; 2.College of Life Sciences,Jiangxi Key Laboratory of Bioprocess,Jiangxi Science and Technology Normal University,Nanchang Jiangxi 330038,China
关键词:
环二肽;  放线菌;  盐胁迫;  次级代谢产物
Keywords:
cyclodipeptides;  actinomycetes;  salt stress;  secondary metabolites
分类号:
Q 93
DOI:
10.16357/j.cnki.issn1000-5862.2022.03.05
文献标志码:
A
摘要:
该文选择陆生放线菌Streptomyces albovinaceus DSM 40136,结合菌株肽类次级代谢产物生物合成基因簇的分析,利用盐胁迫策略对其进行产物发掘,成功地利用添加海盐质量分数为3%的M-ISP4培养基激活菌株生产出具有多种生物学活性的环二肽Cyclo(L-Pro-L-Leu).该产物的发掘表明:利用海盐胁迫策略可有效应用于陆生放线菌次级代谢潜能的激活,且新产物的发掘也为环二肽生物合成研究及代谢工程改造提供了良好的出发菌株.
Abstract:
In this paper,the Streptomyces albovinaceus DSM 40136 with detail genomic sequence is selected to cultivated by salt stress.After the comparison analysis of the fermentation extract,the strain can be activated to produce Cyclo(L-Pro-L-Leu)in the cultivated by M-ISP4 medium added 3% sea salt.This activation of natural product biosynthesis indicates that the feasible of salt stress applies in secondary metabolites development derived from terrestrial actinomycetes.Additionally,the S. albovinaceus DSM 40136,new Cyclo(L-Pro-L-Leu)producer,are also new strain deserved in future biosynthesis mechanism research and metabolic engineering modification.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2021-12-20
基金项目:国家自然科学基金(82160671,32060021)和江西省自然科学基金(20202BAB203021)资助项目.
通信作者:朱 笃(1971—),男,江西高安人,教授,博士,主要从事生物过程工程、次级代谢调控研究.E-mail:zhudu12@163.com
谢运昌(1984—),男,江西赣州人,讲师,博士,主要从事放线菌次级代谢产物的组合生物合成与基因组挖掘研究.E-mail:xieyunchang1984@sina.com
更新日期/Last Update: 2022-05-25