[1]赖 胜,杨慧林,陈美玲,等.鄱阳湖南矶湿地苔草植被根际土壤微生物群落结构分析[J].江西师范大学学报(自然科学版),2023,(01):82-90.
　LAI Sheng,YANG Huilin,CHEN Meiling,et al.The Analysis of Microbial Community Structure in Rhizosphere Soil of Carex Vegetation in Poyang Lake Nanji Wetland[J].Journal of Jiangxi Normal University:Natural Science Edition,2023,(01):82-90.
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鄱阳湖南矶湿地苔草植被根际土壤微生物群落结构分析()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年01期
页码:
82-90
栏目:
生命科学
出版日期:
2023-01-25

文章信息/Info

Title:
The Analysis of Microbial Community Structure in Rhizosphere Soil of Carex Vegetation in Poyang Lake Nanji Wetland
作者:
赖 胜1; 杨慧林1; 2; 陈美玲1; 龙馨怡1; 刘淑丽2; 简敏菲1; 2*
(1.江西师范大学江西省亚热带植物资源保护与利用重点实验室,江西 南昌 330022; 2.江西师范大学鄱阳湖湿地与流域研究教育部重点实验室,江西 南昌 330022)
Author(s):
LAI Sheng1; YANG Huilin1; 2; CHEN Meiling1; LONG Xinyi1; LIU Shuli1; 2; JIAN Minfei1; 2*
(1.Key Lab of Protection and Utilization of Subtropical Plant Resources,Jiangxi Normal University,Nanchang Jiangxi 330022,China; 2.Key Laboratory of Ministry of Education of Lake Poyang Wetland and Watershed Research,Jiangxi Normal University, Nanchang Jiangxi 330022,China)
关键词:
鄱阳湖南矶湿地;  高通量测序;  土壤微生物;  群落结构
Keywords:
Poyang Lake Nanji Wetland;  high-throughput sequencing;  soil microorganisms;  community structure
分类号:
Q 93
文献标志码:
A
摘要:
为探究鄱阳湖南矶湿地苔草植被根际土壤微生物群落结构,该文以鄱阳湖南矶湿地苔草植被根际土壤为研究对象,采取0～15 cm、15～30 cm及30～45 cm深度的土壤,采用Illumina HiSeq高通量测序技术,研究不同深度土壤群落结构及其影响因素.研究结果表明:不同深度土壤样品理化性质和酶活性存在显著差异,随土壤深度的增加呈降低趋势.Pearson相关分析表明鄱阳湖南矶湿地苔草植被根际土壤理化性质与土壤酶活性之间存在不同程度的相关性.高通量测序结果表明不同深度土壤细菌和真菌群落微生物丰富度及多样性未达到在统计学意义上的差异显著水平(P>0.05),土壤古菌群落微生物多样性随土壤深度的增加而增加.在苔草植被根际土壤中共检测到41个细菌门类,其中变形菌门、酸杆菌门、绿弯菌门和硝化螺旋菌门为优势菌门; 检测到8个真菌门类,其中子囊菌门、担子菌门和罗兹菌门为优势菌门; 检测到10个古菌门类,其中广古菌门、泉古菌门、奇古菌门为优势菌门.冗余分析表明:土壤有机质、全氮、全磷和碱解氮含量是影响细菌群落的主要环境因子; 土壤有机质、全氮、有效磷和碱解氮含量是影响真菌群落的主要环境因子; 土壤有机质、全氮、全磷和全钾含量是影响古菌群落的主要环境因子.
Abstract:
In order to explore the effects of microbial community structure in rhizosphere soil of Carex vegetation in Poyang Lake Nanji Wetland,Illumina HiSeq high-throughput sequencing technology is applied to examine the soil community structure and its influencing factors at the layer of 0—15,15—30 and 30—45 cm of Carex vegetation soil in Nanji Wetland.The results show that there are significant differences in the physical and chemical properties and enzyme activities of soil samples at different depths.Pearson correlation analysis shows that there are varying degrees of correlation between them.High-throughput sequencing results show that the microbial richness and diversity of soil bacterial and fungal communities at different depths did'n reach significant differences(P>0.05)and archaeal soil microbial diversity increases with soil depth.A total of 41 bacterial phyla,8 fungal phyla and 10 archaea phyla are obtained from different treatments,of which the dominant bacterial phyla are Proteobacteria,Acidobacteria,Chloroflexi and Nitrospirae,the dominant fungal phyla are Ascomycota,Basidiomycota and Mortierellomycota,the dominant archaea phyla are Euryarchaeota,Crenarchaeota and Thaumarchaeota.The redundancy analysis shows that organic matter,total nitrogen,total phosphorus and alkaline hydrolyzed nitrogen have greater impact on bacterial community structure,while organic matter,total nitrogen,available phosphorus and alkali hydrolyzable nitrogen content have greater impact on fungal community structure.And organic matter,total nitrogen,total phosphorus and total potassium content have greater impact on archaea community structure.

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备注/Memo

备注/Memo:
收稿日期:2022-08-30
基金项目:国家自然科学基金(42007389),江西省自然科学基金(20181BAB214003),江西省水利科学院江西省鄱阳湖水资源与环境重点实验室开放研究基金(2020GPSYS03)和江西省教育厅研究生创新基金(YC2022-s281)资助项目.
通信作者:简敏菲(1969—),女,江西高安人,教授,博士,博士生导师,主要从事土壤污染方面相关研究.E-mail:jianminfei@jxnu.edu.cn
更新日期/Last Update: 2023-01-25