[1]杨喜艳,周天寿.一个多ON基因系统的表达动力学分析[J].江西师范大学学报(自然科学版),2016,40(01):5-14.
 YANG Xiyan,ZHOU Tianshou.The Analysis of Expression Dynamics in a Multi-On Gene System[J].,2016,40(01):5-14.
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一个多ON基因系统的表达动力学分析()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
40
期数:
2016年01期
页码:
5-14
栏目:
出版日期:
2016-01-25

文章信息/Info

Title:
The Analysis of Expression Dynamics in a Multi-On Gene System
作者:
杨喜艳;周天寿
1.广东金融学院应用数学系,广东 广州 510521; 2.中山大学数学与计算科学学院,广东 广州 510275
Author(s):
YANG Xiyan ZHOU Tianshou
1.Department of Applied Mathematics,Guangdong University of Finance,Guangzhou Guangdong 510521,China; 2.School of Mathematics and Computational Science,Sun Yat-Sen University,Guangzhou Guangdong 510275,China
关键词:
多ON基因系统 总作用效能 mRNA分布 表达噪声 爆发动力学
Keywords:
multi-on model total interaction efficiency mRNA distribution expression noise bursting kinetics
分类号:
O 242; Q 141; Q 332
文献标志码:
A
摘要:
研究了一个转录水平上的基因表达模型,它不仅考虑了启动子的复杂性,而且考虑了相互作用转录因子的调控效果.假定启动子区域有2个调控位点,启动子有1个失活态和2个激活态(即2个不同的转录出口),并考虑了绑定到不同调控位点的转录因子之间相互作用的3种代表性机制:招募机制,稳定机制和混合机制.理论分析和数值模拟结果显示:不同的转录出口能够导致mRNA分布峰的多样性; 3种机制下的平均表达水平和平均爆发频率都相同,但稳定机制诱导最大的表达噪声和平均爆发规模,而招募机制诱导最小的表达噪声和平均爆发规模.这些结果表明:细胞表型的产生源是复杂的,既与启动子结构有关也与调控机制有关.
Abstract:
The relationship between cellular phenotype across a genetically identical population and gene expression difference has being a very active topic.Here,a gene expression model at the transcription level is studied,considering not only complexity of promoter structure but also interactive effect between transcription factors.The promoter region is assumed to contain two regulatory sites and the promoter has one inactive state and two active states(i.e.,two different transcription exits).In addition,based on interaction between transcription factors binding to the different regulatory sites,three representative mechanisms are considered,including recruitment mechanism,stabilization mechanism and mixture mechanism.Theoretical analysis and numerical simulation results show that different transcription exits can lead to mRNA distribution peaks of different modes; the mRNA mean level and mean burst frequency are identical under these three mechanisms,but stabilization mechanism induces the highest expression noise and the largest mean burst size,and recruitment mechanism induces the lowest expression noise and the smallest mean burst size.These results indicate that the source of cellular phenotype is complex,which is related to both promoter structure and regulation mechanisms.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(91230204,11005162,11475273,11526058,2014CB964703),2015年广东省教育厅青年创新人才类(2015KQNCX103)和2015年广东金融学院“创新强校工程”(高校重大科研成果培育计划类)资助项目.
更新日期/Last Update: 1900-01-01