[1]肖小军,张慧云,林瑛姿,等.1,25(OH)_2VD_3在过敏原特异性免疫治疗花粉过敏哮喘中的应用[J].江西师范大学学报(自然科学版),2016,40(02):186-189.
 XIAO Xiaojun,ZHANG Huiyun,LIN Yingzi,et al.The Effects of 1,25(OH)2VD3 for Allergen Specific Immunotherapy in Allergic Asthma[J].,2016,40(02):186-189.
点击复制

1,25(OH)_2VD_3在过敏原特异性免疫治疗花粉过敏哮喘中的应用()
分享到:

《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
40
期数:
2016年02期
页码:
186-189
栏目:
出版日期:
2016-03-25

文章信息/Info

Title:
The Effects of 1,25(OH)2VD3 for Allergen Specific Immunotherapy in Allergic Asthma
作者:
肖小军;张慧云;林瑛姿;刘志刚;杨平常;刘晓宇
1.深圳大学医学院过敏反应与免疫学研究所,广东 深圳 518060; 2.海南医学院病理生理学教研室,海南 海口 571101
Author(s):
XIAO XiaojunZHANG HuiyunLIN YingziLIU ZhigangYANG PingchangLIU Xiaoyu
1.Institute of Allergy and Immunology,School of Medicine,Shenzhen University,Shenzhen Guangdong 518060,China; 2.Department of Pathophysiology,Hainan Medical College,Haikou Hainan 571101,China
关键词:
125(OH)2VD3 特异性免疫治疗 过敏哮喘 软叶针葵 profilin
Keywords:
125(OH)2VD3 specific immunotherapy allergic asthma phoenix roebelenii profilin
分类号:
R 392.8
文献标志码:
A
摘要:
以1,25(OH)2VD3为佐剂制备软叶针葵花粉变应原疫苗,以小鼠致敏哮喘模型为研究对象进行特异性免疫治疗.通过检测小鼠气道高反应性、血清中特异性抗体、细胞因子以及肺组织病理学切片等指标对治疗模型的构建进行评价,探讨1,25(OH)2VD3在过敏原特异性免疫治疗花粉过敏哮喘免疫机制中的作用.结果表明:以1,25(OH)2VD3为变应原疫苗能有效抑制花粉特异性IgE的产生和Th2细胞因子IL-4分泌,促进封闭性抗体IgG2a产生和Th1细胞因子IFN-γ的分泌,增加耐受性细胞因子IL-10生产,使Th2反应向Th1反应转变.1,25(OH)2VD3在花粉过敏性哮喘治疗中能大幅提高过敏原特异性免疫治疗的疗效.
Abstract:
BALB/c mice as research allergic asthma models,then immunological therapy with allergen vaccine together with 1,25(OH)2VD3 as adjuvants through subcutaneous injection.Evaluate these treatment models by detecting the mice before and after the treatment of airway hyperresponsiveness,specific antibodies in serum,cytokines and pathological changes of lung.A murine model of allergic asthma was established to study the efficacy and mechanism of the 1,25(OH)2VD3 for the treatment of allergic asthma.After 1,25(OH)2VD3 vaccines specific immune therapy,the lung symptoms and high reactivity of mice are decreased,and the levels of IgG2a elevated,IgE levels drop,which are specificity antibody in serum.In supernatant of cultivating spleen cells,INF-γand IL-10 levels increase,the production of IL-4 decreased.1,25(OH)2VD3 functions more appropriately as a specific immunotherapy adjuvant for allergen vaccines due to its superior efficacy and markedly fewer side effects.

参考文献/References:

[1] Liu Zhigang,Song Juanjuan,Kong Xiaoli.A study on pollen allergens in China [J].Biomedical and Environmental Sciences,2010,23:319-322.
[2] 刘志刚,易海涛,夏立新,等.低致敏原应用于特异性免疫治疗的研究进展 [J].江西师范大学学报:自然科学版,2011,35(4):353-357.
[3] Casquete-Roman E,Rosado-Gil T,Postigo I,et al.Profilin cross-reactive panallergen causes latex sensitization in the pediatric population allergic to pollen [J].Annals of Allergy Asthma & Immunology,2012,109(3):215-219.
[4] Xiao Xiaojun,Zhang Xinxin,Ma Li,et a1.Effects of Caryota mitis profilin-loaded PLGA nanoparticles in a murine model of allergic asthma [J].Int J Nanomedicine,2013,8(11):4553-4562.
[5] Liu Xiaoyu,Xiao Xiaojun,Xia Lixin,et a1.Identification and characterization of a pan-allergen profilin,a major allergen from Caryota mitis pollen [J].Asian Pacific Journal of Allergy and Immunology,2014,32(1):39-45.
[6] 孟光,姚敏,刘志刚,等.软叶针葵花粉肌动蛋白抑制蛋白基因的克隆表达与纯化及免疫学鉴定 [J].中华检验医学杂志,2008,31(10):1171-1172.
[7] 冯明礼,赵锦云,刘建珍,等.致敏花粉与变应性鼻炎发病关系及花粉变应原免疫治疗效果的研究 [J].中国公共卫生,2000,16(8):714-715.
[8] 蒋聪利,肖小军,马莉,等.软叶针葵花粉疫苗治疗小鼠过敏性鼻炎的效果和机制 [J].细胞与分子免疫学杂志,2014,30(2):113-116.
[9] 孟光,李春林,蔡琼香,等.海口地区热带植物花粉致敏性调查 [J].临床耳鼻咽喉科杂志,2005,19(22):1044-1045.
[10] 宋颖芳,赖国祥,戚好文,等.1,25-二羟维生素D3抑制被动致敏人气道平滑肌细胞的增殖 [J].中国病理生理杂志,2011,27(9):1828-1831.
[11] 解童玲,林荣军,郭建萍,等.1,25二羟基维生素D3对支气管哮喘小鼠白细胞介素-17表达的影响 [J].中华实用儿科临床杂志,2014,29(4):307-310.
[12] 朱剑萍,姚红伊,谢诒诚,等.艾蒿花粉诱导豚鼠过敏反应 [J].中国病理生理杂志,2009,25(1):7-12.
[13] González-Rioja R,Ferrer A,Arilla M C,et al.Diagnosis of Parietaria judaica pollen allergy using natural and recombinant Par j1 and Par j2 allergens [J].Clin Exp Allergy,2007,37(2):243-250.
[14] Nivenka P Jayasekera,Tudor P Toma,Andrew Williams,et al.Mechanisms of immunotherapy in allergic rhinitis [J].Biomedicine & Pharmacotherapy,2007,61(1):29-33.
[15] 万远芳,刁庆春.花粉疫苗治疗变态反应性疾病进展 [J].国际皮肤性病学杂志,2008,34(2):107-109.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81302553),广东省工程技术研究开发中心(2013158925),广东省科技计划(2014A020212466)和深圳市南山区创新机构提升(KC2014ZDTS0004B,KC2014ZDTS0004B)资助项目.
更新日期/Last Update: 1900-01-01