[1]赵思颖,倪才英,符文昌,等.鄱阳湖流域底泥微生物对环境变量的响应[J].江西师范大学学报(自然科学版),2016,40(02):194-199.
 ZHAO Siying,NI Caiying,FU Wenchang,et al.The Microbial Response to Environmental Parameters of the Sediment in Poyang Lake Basin[J].,2016,40(02):194-199.
点击复制

鄱阳湖流域底泥微生物对环境变量的响应()
分享到:

《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
40
期数:
2016年02期
页码:
194-199
栏目:
出版日期:
2016-03-25

文章信息/Info

Title:
The Microbial Response to Environmental Parameters of the Sediment in Poyang Lake Basin
作者:
赵思颖;倪才英;符文昌;王金凤
1.江西师范大学鄱阳湖湿地与流域研究教育部重点实验室,地理与环境学院,江西 南昌 330022; 2.江西师范大学江西省亚热带植物资源保护与利用重点实验室,生命科学学院,江西 南昌 330022
Author(s):
ZHAO SiyingNI CaiyingFU WenchangWANG Jinfeng
1.Key Laboratory of Lake Poyang Wetland and Waershed Research,Ministry of Educatien,Jiangxi Normal University,Nanchang Jiangxi 330022,China; 2.Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province, Jiangxi Normal University,College of Life Science,Nanchang Jiangxi 330022,China
关键词:
底泥 微生物 16S rRNA 重金属 鄱阳湖流域
Keywords:
sediment microbes heavy metals 16S rRNA Poyang Lake basin
分类号:
X 552; Q 114
文献标志码:
A
摘要:
以鄱阳湖流域4个支流共6个地点(信江XS1、信江下水湾XS3、饶河RS、饶河内湖RHS、修水SS、赣江GS)的底泥样品为研究对象,利用16 S rRNA基因扩增技术探究底泥微生物对环境变量的响应.结果显示:6个采样点的底泥样品的微生物群落结构大致分为4类; 底泥的pH值、TOC、TN、TP和重金属含量对微生物群落结构及微生物多样性存在着显著的影响.4条河流底泥微生物多样性指数顺序为赣江>信江>修水>饶河,同时发现饶河的Cu、Zn含量在4条河流中最高,这说明高Cu、Zn会对微生物多样性产生负面的影响; 河流与其所对应湖区底泥样品中微生物多样性指数比较发现:饶河>饶河内湖,信江下水湾>信江,这种结果顺序的不一致主要是由底泥中营养物质含量不同和某些重金属元素胁迫导致的.
Abstract:
Based on six sites in different tributaries of Poyang lake basin sediment samples,this study makes an attempt to investigate sediment microbial response to environment variablesthe through 16S rRNA gene amplification technique was used to study the microbial community and diversity in different sediment samples.Results showed that 6 samples(Xinjiang XS1,Xinjiang water bay XS3,Raohe RS,Raohe lake RHS,Xiushui SS,Ganjiang GS)of sediment can be divided into four categories.Analysis found that pH and TOC,TN,TP,heavy metal content of the sediment exists significant influence on microbial community structure and microbial diversity,which causes the difference of the microbial community structure and diversity Further analysis shows that the microbial diversity index of four rivers sediment,Ganjiang>Xinjiang>Xiushui>Raohe,addition,Cu and Zn content of Raohe is the highest in the four rivers,illustrates the rises with the content of Cu and Zn increasing,which would have a negative impact on microbial diversity; the difference of microbial diversity index between different river and its corresponding lake sediment samples,Raohe>Raohe lake,Xinjiang water bay>Xinjiang,the difference is mainly casued by the difference content of nutrient in the sediment and some heavy metals stress.

参考文献/References:

[1] 曹铭昌,乐志芳,雷军成,等.全球生物多样性评估方法及研究进展 [J].生态与农村环境学报,2013,29(1):8-16.
[2] 郑璐,尹华群,曹琳辉,等.德兴铜矿酸性矿坑水水样与底泥中微生物群落多样性及其群落结构变化 [J].生态学报,2008,28(10):4841-4848.
[3] 杨磊,胥峥,林逢凯,等.城市富营养化河道底泥中微生物的新陈代谢能力研究 [C].中国环境科学学会学术年会优秀论文集,2006.
[4] Luo Mingbiao,Li Hianqiang,Cao Weipeng,et al.Study of heavy metal speciation in branch sediments of Poyang Lake [J].Environmental Sciences,2008,20(1):161-166.
[5] 蒋先军,骆永明.重金属污染土壤的微生物学评价 [J].土壤,2000,32(3):130-134.
[6] 郭朝晖,廖柏寒.模拟酸雨下 Cd、Cu、Zn复合污染对土壤微生物量碳和酶活性的影响 [J].应用与环境生物学报,2003,9(4):382-385.
[7] Liu Yurong,Wang Jianjun,Zheng Yuanming,et al.Patterns of bacterial diversity along a long-term mercury-contaminated gradient in the paddy soils [J].Microbial Ecology,2014,68(3):575-583.
[8] Rademacher A,Zakrzewski M,Schluter A,et al.Characterization of microbial biofilms in a thermophilic biogas system by high-throughput metagenome sequencing [J].FEMS Microbiol Ecol,2012,79(3):785-799.
[9] 李博.生态学 [M].北京:高等教育出版社,2000.
[10] Westerholm M,Roos S,Schnurer A.Syntrophaceticus schinkii gen.nov.,sp.nov.,an anaerobic,syntrophic acetate-oxidizing bacteriumisolated from a mesophilic anaerobic filter [J].FEMS Microbiology Letters,2010,309(1):100-104.
[11] 李家宝,芮俊鹏,张时恒,等.原核微生物菌群的空间分异增强秸秆-猪粪混合发酵效率 [J].化工学报,2014,65(5):1792-1799.
[12] 南京农业大学.土壤农业化学分析 [M].2版.北京:中国农业出版社,1990.
[13] 陶勇,芮俊鹏,李家宝,等.浓香型白酒窖泥中细菌和古菌的组成与多样性 [J].化工学报,2014,65(5):1800-1807.
[14] Edgar R C,Haas B J,Clemente J C,et al.UCHIME improves sensitivity and speed of chimera detection [J].Bioinformatics,2011,27(16):2194-2200.
[15] 芮俊鹏,李吉进,李家宝,等.猪粪原料沼气工程系统中的原核微生物群落结构 [J].化工学报,2014,65(5):1868-1875.
[16] Edgar R C.Search and clustering orders of magnitude faster than BLAST [J].Bioinformatics,2010,26(19):2460-2461.
[17] Islam E,Paul D,Sar P.Microbial diversity in uranium deposits from Jaduguda and Bagjata uranium mines,India as revealed by Clone Library and Denaturing Gradient Gel electrophoresis [J].Analyses Geomicrobiology Journal,2014,31(10):862-874.
[18] 谢学辉,范凤霞,袁学武,等.德兴铜矿尾矿重金属污染对土壤中微生物多样性的影响 [J].微生物学通报,2012,39(5):624-637.
[19] 张萍华,申秀英,许晓路,等.对白术土壤微生物及酶活性的影响 [J].土壤通报,2005,36(2):227-229.
[20] 陈灵芝,马克平.生物多样性科学:原理与实践 [M].上海:上海科学技术出版社,2001.
[21] 弓晓峰,陈春丽,周文斌,等.鄱阳湖底泥中重金属污染现状评价 [J].环境科学,2006,27(4):732-736.
[22] 鄱阳湖研究编委会.鄱阳湖研究 [M].上海:上海科学技术出版社,1988.
[23] 李鸣,刘琪璟.鄱阳湖水体和底泥重金属污染特征与评价 [J].南昌大学学报:理学版,2010,34(5):486-489.
[24] 张静霞.湘江株洲段典型重金属污染区底泥微生物群落结构多样性及其生物浸出的研究 [D].长沙:中南大学,2011.
[25] 吴建军,蒋艳梅,吴喻萍,等.重金属复合污染对水稻土微生物生物量和群落结构的影响 [J].土壤学报,2008,45(6):1102-1109.
[26] 陈素华,孙铁珩,周启星,等.微生物与重金属间的相互作用及其应用研究 [J].应用生态学报,2002,13(2):64-65.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金(41061037),鄱阳湖湿地与流域研究教育部重点实验室(江西师范大学)主任基金(ZK2013007,ZK2013001)和鄱阳湖湿地与流域研究教育部重点实验室(江西师范大学)开放基金(PK2012006)资助项目.
更新日期/Last Update: 1900-01-01