[1]叶树虹,郑文平,赵素彩,等.新型凝血酶电化学传感器的制备[J].江西师范大学学报(自然科学版),2012,(02):180-184.
 YE Shu-hong,ZHENG Wen-ping,ZHAO Su-cai,et al.The Preparation of the Novel Electrochemical Biosensor for Thrombin Detection[J].Journal of Jiangxi Normal University:Natural Science Edition,2012,(02):180-184.
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新型凝血酶电化学传感器的制备()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2012年02期
页码:
180-184
栏目:
出版日期:
2012-03-01

文章信息/Info

Title:
The Preparation of the Novel Electrochemical Biosensor for Thrombin Detection
作者:
叶树虹;郑文平;赵素彩;曾丽;余琦;李成;宋永海;汪莉
江西师范大学化学化工学院,江西南昌,330022
Author(s):
YE Shu-hong;ZHENG Wen-ping;ZHAO Su-cai;ZENG Li;YU Qi;LI Cheng;SONG Yong-hai;WANG Li
关键词:
核酸适配体金纳米颗粒凝血酶传感器
Keywords:
aptamer AuNPs thrombin biosensor
分类号:
O646
文献标志码:
A
摘要:
制备了金纳米粒子-核酸适体/凝血酶/巯基六醇修饰的金电极,测试了该修饰电极检测凝血酶含量的性能指标.利用电化学技术研究了电极的构造对检测凝血酶含量的影响,探究了最佳实验条件.研究结果表明,在最佳的实验条件下构筑的修饰电极检测凝血酶的线性范围为1.0~10.0 nmol/L,检测限为0.2 nmol/L,并模拟了实际样品进行检测.该方法简便迅速,有较高的检测灵敏度、较好的重复性和抗干扰性.
Abstract:
This work prepared an AuNPs-aptamer/thrombin/6-mercapto-1-hexanol modified Au electrode and measured some parameters for the electrochemical detection of thrombin. Electrochemical technique was used to investigate the construction of the modified electrode and its effects on thrombin detection and explored the optimal experiment conditions. The results indicated that the modified electrode showed a good linear relationship between the peak current and the concentration of thrombin in the range of 1.0~10.0 nmol/L with a detection limit of 0.2 nmol/L. And the modified electrode was applied in the detection of analogous thrombin samples. It was found that the resulted sensor had high sensitivity, good reproducibility and anti-interference.

参考文献/References:

[1] Tu Yonghua, Cheng Guifang, Lin Li, et al. Detection of thrombin with a novel nanobiosensor based on aptamer fluorescence [J]. Chem J Chinse U, 2006, 27(12): 2266-2270.
[2] Zheng Jing, Cheng Guifang, He Pingang, et al. An aptamer-based assay for thrombin via structure switch based on gold nanoparticles and magnetic nanoparticles [J]. Talanta, 2010, 80 (5): 1868-1872.
[3] Tao Bing, Liu Xiangjun, Cheng Xiaohong, et al. Bifunctional combined aptamer for simultaneous separation and detection of thrombin [J]. Biosens Bioelectron, 2010, 25(6): 1487-1492.
[4] Wu Zaisheng, Guo Mengmeng, Zhang Songbai, et al. Reusable electrochemical sensing plateform for highly sensitive detection of small molecules based on structure-switching signaling aptamers [J]. Anal Chem, 2007, 79(7): 2933-2939.
[5] Mir M, Vreeke M, Katakis I. Different strategies to develop an electrochemical thrombin aptasensor [J]. Electrochem Commun, 2006, 8(3): 505-511.
[6] Wu Zaisheng, Zheng Fan, Shen Guoli, et al. A hairpin aptamer-based electrochemical biosensing platform for the sensitive detection of proteins [J]. Biomatrials, 2009, 30(15): 2950-2955.
[7] Tombellis S, Minunni M, Mascini M. Analytical application of Aptamers [J]. Biosens Bioelectron, 2005, 20(12): 2424-2434.
[8] Cai Hong, Lee Thomas Minghung, Hsing I-Ming. Label-free protein recognition using an aptamer-based impedance measurement assay [J]. Sens Actuat B, 2006, 114: 433-437.
[9] Bini A, Minunni M, Tombelli S, et al. Analytical performance of aptamer-based sensing for thrombin detection [J]. Anal Chem, 2007, 79(7): 3016-3019.
[10] He Pingli, Shen Li, Cao Yunhe, et al. Ultrasensitive electrochemical detection of proteins by amplification of aptamer-nanoparticle bio bar codes [J]. Anal Chem, 2007, 79(21): 8024-8029.
[11] Huang Haiping, Zhu Junjie. DNA aptamer-based QDs electrochemilu?minescence biosensor for the detection of thrombin [J]. Biosens Bioelectron, 2009, 25(4): 927-930.

备注/Memo

备注/Memo:
国家自然科学基金(20905032;21065005);江西省自然科学基金(2008GZH0028);江西省教育厅科技项目(GJJ10389);电分析化学国家重点实验室基金(2008003);江西师范大学大学生学术课题资助项目
更新日期/Last Update: 1900-01-01