[1]李钟鸣,邬玉兰,刘志刚.粉尘螨Der f15的基因克隆与其表达载体的构建[J].江西师范大学学报(自然科学版),2013,(02):159-161.
　LI Zhong-ming,WU Yu-lan,LIU Zhi-gang.Cloning and Vectorconstuction of Der f15 from House Dust Mite Dermatophagoides Farina[J].Journal of Jiangxi Normal University:Natural Science Edition,2013,(02):159-161.
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粉尘螨Der f15的基因克隆与其表达载体的构建()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2013年02期
页码:
159-161
栏目:
出版日期:
2013-03-01

文章信息/Info

Title:
Cloning and Vectorconstuction of Der f15 from House Dust Mite Dermatophagoides Farina
作者:
李钟鸣;邬玉兰;刘志刚
深圳大学医学院过敏反应与免疫学研究所,广东深圳,518060
Author(s):
LI Zhong-ming；WU Yu-lan；LIU Zhi-gang
关键词:
粉尘螨; Der f15; 克隆; 载体构建
Keywords:
dermatophagoides farina; Der f15; cloning; vectorconstruction
分类号:
Q78
文献标志码:
A
摘要:
先挑取纯培养的粉尘螨,提取总的RNA,后采用RT-PCR方法进行反转录出cDNA.由cDNA提取出目的基因进行片段扩增,产物连接入T载体(pMD18-T).经扩增后,用EcoR I和Xho I双酶切,将目的基因分别连接到pET28和pET32的表达载体上,得到重组质粒pET28和pET32,即粉尘螨的基因克隆与 表达载体构建成功.
Abstract:
The living mites of south China area which had been identified and cultured were picked,from which extracted the total RNA.RT-PCR technology were used to clone the gene and then connected it to the T vector(pMD18-T).After that,the expression vector(pET28 and pET32)was constructed for the new gene segment and were connect ed together.

参考文献/References:

[1] 刘志刚,刘瑞涛,赖仞,等.粉尘螨抗细菌活性物质的分离与鉴定 [J].江西师范大学学报:自然科学版,2011,35(1):34-36.
[2] 刘晓宇,张强,吉坤美,等.粉尘螨疫苗免疫治疗过敏性鼻炎小鼠鼻粘膜的电镜观察 [J].江西师范大学学报:自然科学版,2011,35(5):532-536.
[3] Platts-Mills T A,Thomas W R,Aalberse R C,et al.Dust mite allergens and asthma:Report of a second international workshop [J].Allergy Clin Immunol,1992,89:1046-1060.
[4] 徐瑞霞,蒋鹤龄.尘螨浸液治疗31例变态反应性疾病疗效观察 [J].安徽预防医学杂志,1997,3(1):68-69.
[5] Sporick R,Holgate S,Platts-Mills T,et al.Exposure to house dust mite allergen(Der p1)and the development of asthma in childhood N [J].Engl J Med,1990,323:502-507.
[6] Sture G.Canine atopic disease:the prevelance of positive intradermal skin tests at two sites in the north and south of Great Britain [J].Vet Immunol Immunopathol,1995,44(3/4):293-308.
[7] 郝敏麒,徐军,钟南山.华南地区粉尘螨主要变应原Der f2的cDNA克隆及序列分析 [J].中国寄生虫学与寄生虫病杂志,2003,21(3):160-163.
[8] McCall C,Hunter S,Stedman K,et al.Characterization and cloning of a major high molecular weight house dust mite allergen(Der f15)for dog [J].Vet Immunol Immunopathol,2001,78:231-247.
[9] Kyoung Yong Jeong,Jung-Won Park,Chein-Soo Hong.House dust mite allergy in Korea:The most important inhalant allergen in current and future [J].Allergy Asthma Immunol Res,2012,4(6):313-325.

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备注/Memo

备注/Memo:
国家"863"计划(2006AA02A231)；国家自然科学基金(81071388)；广东省自然科学基金(04554)；广东省高等学校国际暨港澳台科技合作创新平台项目(2012gjhz0009)；深圳市重点实验室组建项目(SW201110010)；深圳市科技计划基础研究重点项目(JCYJ20120613100657482)；深圳市南山区一般研发课题(KC2012JSYB0003A)
更新日期/Last Update: 1900-01-01