[1]黄 倩,涂艺声*,余 晓,等.不同色光对蛇足石杉离体叶状体产HupA及内源激素水平的影响[J].江西师范大学学报(自然科学版),2019,(03):248-252.[doi:10.16357/j.cnki.issn1000-5862.2019.03.06]
　HUANG Qian,TU Yisheng*,YU Xiao,et al.The Effects of Different Colored Light on HupA Accumulation and Endogenous Hormone Content Produced by the Thallus of Huperzia serrata in vitro[J].Journal of Jiangxi Normal University:Natural Science Edition,2019,(03):248-252.[doi:10.16357/j.cnki.issn1000-5862.2019.03.06]
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不同色光对蛇足石杉离体叶状体产HupA及内源激素水平的影响()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年03期
页码:
248-252
栏目:
化学与生命科学
出版日期:
2019-06-10

文章信息/Info

Title:
The Effects of Different Colored Light on HupA Accumulation and Endogenous Hormone Content Produced by the Thallus of Huperzia serrata in vitro
文章编号:
1000-5862(2019)03-0248-05
作者:
黄 倩; 涂艺声*; 余 晓; 袁慧慧
江西师范大学生命科学学院,江西 南昌 330022
Author(s):
HUANG Qian; TU Yisheng*; YU Xiao; YUAN Huihui
College of Life Science,Jiangxi Normal University,Nanchang Jiangxi 330022,China
关键词:
蛇足石杉;  不同色光;  生长;  石杉碱甲;  内源激素
Keywords:
Huperzia serrata;  different colored lights;  growth;  Huperzine A(HupA);  endogenous hormone
分类号:
R 931.2
DOI:
10.16357/j.cnki.issn1000-5862.2019.03.06
文献标志码:
A
摘要:
为探究不同色光对蛇足石杉离体叶状体生长和石杉碱甲(HupA)累积的作用,设置了二极管白光(W)、二极管蓝光(B)、二极管红光(R)3种不同色光处理,以普通荧光灯(CK)为对照,研究不同色光下叶状体生长和HupA累积的差异及其与IAA、ZT、ABA、GA几种内源激素水平的关系.结果表明:红光处理对蛇足石杉离体叶状体生长繁殖有促进效果,但对HupA含量累积有极显著的抑制作用; 蓝光相较CK提高了叶状体的生长量和HupA的含量,其叶状体HupA生产率最高达1 811.728 μg·L-1,较CK提高了49.67%; 蓝光处理的叶状体IAA/ZT值、ABA含量、ABA/ZT值、ABA/IAA值在全生育期均高于CK.依据不同类型激素功能,该研究结果说明:蓝光培养的叶状体中存在高水平的生长型激素IAA/ZT,促进其生长,增加了生物量,且其体内成熟型激素ABA的高水平存在,促进了次级代谢产物HupA累积增加.
Abstract:
For exploring the effect of different colored lights on the growth and Huperzine A accumulation of the thallus of Huperzia serrata,set up several different colored lights treatment including diode white light(W),diode blue light(B),diode red light(R)are set up to treat.Compared with the common fluorescent lamp(CK),and studied the difference of the growth and HupA accumulation of the thallus under different colored lights and the relationship between them and endogenous hormone including IAA,ZT and ABA,GA are studied.The results show that red light is helpful to promote the growth and proliferation of the thallus of Huperzia serrata in vitro,but it had an extremely significant inhibitory effect on content of HupA.Compared with CK,blue light treatment increase the growth amount and HupA content of the thallus, and the HupA productivity is highest,up to 1 811.728 μg·L-1,which is higher 49.67% than CK.The total IAA/ZT value,the ABA content,ABA/ZT value,ABA/TAA value of the thallus treated with diode blue light are all higher than CK during full period.Depending on the function of different types of hormones,the results of this study indicate that there is high level of growth hormone IAA/ZT in the thallus treated with blue light,which promotes its growth and increased the biomass,and the high level of ABA promotes the accumulation of secondary metabolite HupA.

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相似文献/References:

[1]赖政,李希茜,汪涯,等.蛇足石杉中石杉碱甲含量测定及其时空动态[J].江西师范大学学报(自然科学版),2014,(05):489.
　LAI Zheng,LI Xi-xi,WANG Ya,et al.The Determination of Huperzine A and Temporal and Spatial Variation of Huperzia serrata[J].Journal of Jiangxi Normal University:Natural Science Edition,2014,(03):489.

备注/Memo

备注/Memo:
收稿日期:2018-06-10
基金项目:国家自然科学基金(81660597,81360614)和江西省自然科学基金(20132BAB204023)资助项目.
通信作者:涂艺声(1957-),女,江西奉新人,教授,主要从事植物资源与利用研究.E-mail:ysttz2012@163.com
更新日期/Last Update: 2019-06-10