[1]李 翔,廖焰焰,夏婷婷,等.高效脱氮好氧反硝化菌的筛选及其污水处理工艺优化[J].江西师范大学学报(自然科学版),2019,(03):253-259.[doi:10.16357/j.cnki.issn1000-5862.2019.03.07]
 LI Xiang,LIAO Yanyan,XIA Tingting,et al.The Screening of Highly Active Denitrification Aerobic Denitrifying Bacteria and Its Optimization of Wastewater Treatment Process[J].Journal of Jiangxi Normal University:Natural Science Edition,2019,(03):253-259.[doi:10.16357/j.cnki.issn1000-5862.2019.03.07]
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高效脱氮好氧反硝化菌的筛选及其污水处理工艺优化()
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《江西师范大学学报》(自然科学版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年03期
页码:
253-259
栏目:
化学与生命科学
出版日期:
2019-06-10

文章信息/Info

Title:
The Screening of Highly Active Denitrification Aerobic Denitrifying Bacteria and Its Optimization of Wastewater Treatment Process
文章编号:
1000-5862(2019)03-0253-07
作者:
李 翔廖焰焰夏婷婷王筱兰*
江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西 南昌 330022
Author(s):
LI XiangLIAO YanyanXIA TingtingWANG Xiaolan*
College of Life Science,Key Laboratory of the Conservation and Sustainable Utilization for Subtropical Plant Resources of Jiangxi Province,Jiangxi Normal University,Nanchang Jiangxi 330022,China
关键词:
好氧反硝化菌 脱氮 反硝化作用 氨氮去除率
Keywords:
aerobic denitrifying bacteria denitrification aerobic denitrification ammonia nitrogen removal rate
分类号:
X 172
DOI:
10.16357/j.cnki.issn1000-5862.2019.03.07
文献标志码:
A
摘要:
从活性污泥中驯化、筛选并分离出1株能有效去除氨氮的菌株LX 1-3,经过形态学与分子生物学鉴定该菌株为副球菌属(Paracouccus sp),NCBI GenBank登录号为MH156598.对该菌株进行反硝化性能测试,结果表明该菌培养的最适条件为30 ℃,最适pH值为7.0~7.3,48 h后脱氮率为30.7%.将该菌与1株高耐盐季也蒙毕赤酵母(KX447139)搭配,脱氮效率有显著提高.在好氧条件下,按照好氧反硝化菌与高效耐盐菌1:1的接菌量配比接入污水中,30 ℃反应48 h后,氨氮去除率为86.36%.该研究为提高污水脱氮处理效率提供了有效的方法.
Abstract:
Aerobic denitrifying bacteria has unique advantages in denitrification,which can be used to denitrify wastewater.In this paper,aerobic denitrifying bacteria has been isolated from activated sludge,it can effectively remove ammonia nitrogen called LX 1-3.Through identification of morphology and molecular biology,the strain is identified as Paracoccus sp,an accession number of NCBI GenBank is MH156598.Further studies showed that the optimum condition for the strain growth and aerobic denitrification is temperature 30 ℃,and pH value is between 7.0 and 7.3,its nitrogen removal rate can reach 30.7% after 24 h.Combination of this strain with a known high-lying,salt-tolerant strain significantly increases nitrogen removal efficiency.Under aerobic conditions,the 1:1 ratio of aerobic denitrifying bacteria to highly saline-tolerant Meyerozymaguilliermondii is added to the sewage.After 48 h of reaction at 30 ℃,the ammonia nitrogen removal rate is as high as 86.36%.This study provides a more effective method for improving the efficiency of denitrification treatment of wastewater.

参考文献/References:

[1] 许尚营,张华,李娜,等.一株好氧反硝化菌的鉴定及反硝化特性研究[J].环境科学与技术,2010,33(2):10-13.
[2] Zhao Bin,He Yiliang,Zhang Xiaofan.Nitrogen removal capability through simultaneous heterotrophic nitrification and aerobic denitrification by Bacillus sp LY[J].Environmental Technology,2010,31(4):409-416.
[3] Jian Lu,Qiang Jin,He Yiliang,et al.Simultaneous removal of phenol and ammonium using Serratia sp LJ-1 capable of heterotrophic nitrification-aerobic denitrification[J].Water Air and Soil Pollution,2014,225(9):2125.
[4] Krul J M.Dissimilatory nitrate and nitrite reduction under aerobic conditions by an aerobically and anaerobically grown Alcaligenes sp and by activated sludge[J].Journal of Applied Bacteriology,2010,40(3):245-260.
[5] Robertson L A,Kuenen J G.Aerobic denitrification:a controversy revived[J].Archives of Microbiology,1984,139(4):351-354.
[6] 杨基先,高珊珊,马放,等.一株好氧反硝化细菌的分离鉴定及反硝化能力[J].环境科学学报,2008,28(7):1302-1307.
[7] Daum M,Zimmer W,Papen H,et al.Physiological and molecular biological characterization of ammonia oxidation of the heterotrophic nitrifier Pseudomonas putida[J].Current Microbiology,1998,37(4):281-288.
[8] Lukow T,Diekmann H.Aerobic denitrification by a newly isolated heterotrophic bacterium strain TL1[J].Biotechnology Letters,1997,19(11):1157-1159.
[9] Alcaligenes faecalis,Ottes S,Sckalk J,et al.Hydroxylamine oxidation and subsequent nitrous oxide production by the heterotrophic ammonia oxidizer Alcaligenes faecalis[J].Applied Microbiology and Biotechnology,1999,51(2):255-261.
[10] 辛明秀.反硝化细菌在污水脱氮中的作用[J].微生物学通报,2007,34(4):773-776.
[11] Takaya N,Catalansakairi M A,Sakaguchi Y,et al.Aerobic denitrifying bacteria that produce low levels of nitrous oxide[J].Appl Environ Microbiol,2003,69(6):3152-3157.
[12] 张庆军.纳氏试剂光度法测定水和废水中氨氮关键问题的研究[J].环境工程,2009,27(1):85-88.
[13] 苏俊峰,杨少斐,黄廷林,等.低温贫营养好氧反硝化细菌SY13的反硝化特性[J].城市环境与城市生态,2013,26(6):39-42.
[14] 周莉,李正魁,王易超,等.纯种氨氧化菌短程反硝化特性[J].环境工程学报,2013,7(4):1219-1224.
[15] 韩永和,章文贤,庄志刚,等.耐盐好氧反硝化菌A-13菌株的分离鉴定及其反硝化特性[J].微生物学报,2013,53(1):47-58.
[16] 丁钰,张婷月,黄民生,等.好氧反硝化菌及其在污水处理和环境修复中的研究进展[J].华东师范大学学报:自然科学版,2018,202(6):7-17,73.
[17] 王薇,蔡祖聪,钟文辉,等.好氧反硝化菌的研究进展[J].应用生态学报,2007,18(11):2618-2625.
[18] 郭焱,张召基,陈少华.好氧反硝化微生物学机理与应用研究进展[J].微生物学通报,2016,43(11):2480-2487.
[19] 王永刚,王旭,张俊娥,等.好氧反硝化细菌研究及应用进展[J].工业水处理,2017,37(2):12-17.
[20] 张宇燕,贺根和,陆丽君,等.好氧反硝化菌的筛选鉴定及其脱氮特性研究[J].井冈山大学学报:自然科学版,2014,35(4):1-6.
[21] 曹林,衣学文,董滨,等.活性污泥中异养硝化好氧反硝化菌的分离及其脱氮性能[J].净水技术,2014,33(3):91-95.
[22] Li Ping,Zhang Shan,Liu Deli.Study progress of bacterial aerobic denitrification[J].Journal of Microbiology,2005,25(1):60-64.
[23] Zhang Guanya,Chen Peiqing.Isolation,identification and characteristics of aerobic denitrifying bacteria[J].Journal of Microbiology,2005,25(6):23-25.

备注/Memo

备注/Memo:
收稿日期:2018-09-12
基金项目:国家自然科学基金(31760449)资助项目.
通信作者:王筱兰(1965-),女,江西景德镇人,教授,博士,主要从事环境微生物研究.E-mail:xlwang08@aliyun.com
更新日期/Last Update: 2019-06-10