[1]赵小辉,秦光炯,朱乾华,等.D-色氨酸荧光猝灭法同时测定激动素和6-苄基腺嘌呤[J].江西师范大学学报(自然科学版),2012,(05):534-538.
　ZHAO Xiao-hui,QIN Guang-jiong,ZHU Qian-hua,et al.Simultaneous Determination of 6-Benzylaminopurine and Kinetin Based on D-Trp Fluorescence Guenching[J].Journal of Jiangxi Normal University:Natural Science Edition,2012,(05):534-538.
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D-色氨酸荧光猝灭法同时测定激动素和6-苄基腺嘌呤()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2012年05期
页码:
534-538
栏目:
出版日期:
2012-10-01

文章信息/Info

Title:
Simultaneous Determination of 6-Benzylaminopurine and Kinetin Based on D-Trp Fluorescence Guenching
作者:
赵小辉;秦光炯;朱乾华;冯雪梅;贺薇;杨季冬
长江师范学院化学化工学院, 重庆 408000
Author(s):
ZHAO Xiao-hui;  QIN Guang-jiong;  ZHU Qian-hua;  FENG Xue-mei;  HE Wei;  YANG Ji-dong
关键词:
荧光偏振; 荧光猝灭; 荧光各向异性; 6-苄基腺嘌呤; 激动素; 同时测定
Keywords:
fluorescence polarization; fluorescence guenching;  fluorescence anisotropy;  6-benzylaminopurine;  kinetin;  simultaneous determination
分类号:
O655
文献标志码:
A
摘要:
在pH值为3.00的B-R缓冲溶液中, D-色氨酸均能与6-苄基腺嘌呤(6-BA)和激动素(KT)这2种天然细胞分裂素作用并产生不同强度的荧光猝灭信号.首先利用荧光偏振信号计算获得的荧光偏振度(p)和荧光各向异性(r)来区分6-苄基腺嘌呤和激动素这2种天然细胞分裂素,同时利用荧光猝灭信号和双标准曲线计量分析法对2种天然细胞分裂素进行同时测定,并将该方法应用于市售苦瓜和番茄样品表皮中6-苄基腺嘌呤和激动素的同时测定,结果令人满意.
Abstract:
In pH=3.00 B-R buffer solution, D-Trp could react with natural cytokinins of 6-benzylaminourine and kinetin and produced different fluorescence quenching spectra signals. This article first established the degree of fluorescence polarization(p) and the degree of fluorescence anisotropy(r) based on the measurements of the polarized fuorescence signals to distinguish two natural cytokinins of 6-benzylaminourine and kinetin. Simultaneously based on the fuorescence quenching spectra signals and the double standard curve method, a novel method for simultaneous determination of 6-benzylaminourine and kinetin was developed. The method was applied to simultaneous determination of 6-benzylaminourine and kinetin in balsam pear skin and tomato skin with satisfactory results.

参考文献/References:

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[4] 杨季冬. 用RRS光谱和“同原射线计量分析法”同时测定D-和L-色氨酸对映异构体 [J]. 分析科学学报, 2006, 22(4): 454-457.
[5] 杨季冬. 共振瑞利散射光谱法同时测定α-萘酚和β-萘酚异构体 [J]. 化学通报, 2008(7): 539-544.
[6] 张书然, 杨季冬. 共振瑞利散射光谱法对盐酸异丙嗪与盐酸氯丙嗪的同时测定 [J]. 分析测试学报, 2009, 28(12): 1362- 1367.
[7] 刘丰. 应用荧光探针DPH测定完整细胞膜的流动性 [J]. 南京医科大学学报, 1996(6): 527-529.
[8] 黄振钟, 裹明华, 黄晓宾. 胡椒基荧光酮与镓的显色反应 [J]. 江西师范大学学报: 自然科学版, 2000, 24(1): 54-57.

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更新日期/Last Update: 1900-01-01