[1]黄梅,叶树虹,宋永海.DNA与CdS-COOH-EcoRI复合物相互作用的研究[J].江西师范大学学报(自然科学版),2014,(02):180-186.
　HUANG Mei,YE Shu-hong,SONG Yong-hai.The Study on the Interaction between DNA and CdS-COOH-EcoRI Complex[J].Journal of Jiangxi Normal University:Natural Science Edition,2014,(02):180-186.
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DNA与CdS-COOH-EcoRI复合物相互作用的研究()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2014年02期
页码:
180-186
栏目:
出版日期:
2014-04-30

文章信息/Info

Title:
The Study on the Interaction between DNA and CdS-COOH-EcoRI Complex
作者:
黄梅;叶树虹;宋永海
江西师范大学化学化工学院,江西南昌,330022
Author(s):
HUANG Mei；YE Shu-hong；SONG Yong-hai
关键词:
CdS-COOH; EcoRI; DNA; CdS-COOH-EcoRI复合物; 荧光光谱; 原子力显微镜; 琼脂糖电泳
Keywords:
CdS-COOH; EcoRI; DNA; CdS-COOH-EcoRI complexes; fluorescence spectroscopy; atomic force microscopy; agarose electrophoresis
分类号:
Q523；TB383.1
文献标志码:
A
摘要:
采用荧光光谱、原子力显微镜、琼脂糖凝胶电泳等技术探究CdS-COOH-EcoRI复合物与DNA的相互作用.研究发现,小粒径的CdS-COOH-EcoRI复合物与DNA除了有特异性结合,还发生非特异性结合.DNA相对浓度比较大以及恒温孵育时间较短时,以特异性结合为主;而随着DNA相对浓度的减小以及恒温孵育时间的延长,会伴有非特异性结合.Ca2+存在时,可以通过延长孵育时间“激活”酶切反应,使得DNA被剪切.因此可以通过改变DNA相对浓度和孵育时间来调控EcoRI对DNA的剪切.
Abstract:
In this work,the interaction between CdS-COOH-EcoRI complex and DNA were studied with a combination of fluorescence spectroscopy,atomic force microscopy,agarose gel electrophoresis technology.In the study,it was found that in addition to specific combination there also exists nonspecific combination.Specific combination is dominant in the condition of relatively low DNA concentration and short thermostatic incubation time.However,nonspecific combination also occurs with the decrease of the relative DNA concentration and thermostatic incubation time.If there exists Cacationic in the solution,the incubation time can be prolonged to “activate” enzyme reaction,thus making the DNA sheared.From this,the relative DNA concentration and incubation time were allowed to alter so as control the shearing of DNA by EcoRI.

参考文献/References:

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相似文献/References:

[1]申源,叶树虹,汪莉,等.DNA与CdS-NH_2纳米粒子的相互作用研究[J].江西师范大学学报(自然科学版),2014,(06):624.
　SHEN Yuan,YE Shu-hong,WANG Li,et al.The Study on the Interactions between DNA and CdS-NH2 Nanoparticles[J].Journal of Jiangxi Normal University:Natural Science Edition,2014,(02):624.

备注/Memo

备注/Memo:
国家自然科学基金(20905032,21065005)；江西省教育厅科技项目(GJJ10389)；电分析化学国家重点实验室基金(2008003)
更新日期/Last Update: 1900-01-01