[1]陈世华,董德刚,王思佳,等.山香圆总黄酮对慢性咽炎模型大鼠 NF-κB、IκBα表达的影响[J].江西师范大学学报(自然科学版),2020,(03):259-262.[doi:10.16357/j.cnki.issn1000-5862.2020.03.08]
　CHEN Shihua,DONG Degang,WANG Sijia,et al.The Effect of Total Flavonoids from Turpinia arguta Seem on NF-κB and IκBα Expression in Chronic Pharyngitis Model Rats[J].Journal of Jiangxi Normal University:Natural Science Edition,2020,(03):259-262.[doi:10.16357/j.cnki.issn1000-5862.2020.03.08]
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山香圆总黄酮对慢性咽炎模型大鼠 NF-κB、IκBα表达的影响()
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《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年03期
页码:
259-262
栏目:
化学与生命科学
出版日期:
2020-06-10

文章信息/Info

Title:
The Effect of Total Flavonoids from Turpinia arguta Seem on NF-κB and IκBα Expression in Chronic Pharyngitis Model Rats
文章编号:
1000-5862(2020)03-0259-04
作者:
陈世华; 董德刚; 王思佳; 黎美琦; 胡明明; 黄佩蓓*
江西中医药大学中医学院,江西 南昌 330004
Author(s):
CHEN Shihua; DONG Degang; WANG Sijia; LI Meiqi; Hu Mingming; HUANG Peibei*
College of Traditional Chinese Medicine,Jiangxi University of Traditional Chinese Medicine,Jiangxi Nanchang 330004,China
关键词:
山香圆;  总黄酮;  慢性咽炎;  NF-κB;  IκBα
Keywords:
Turpinia arguta Seem;  total flavonoids;  Chronic pharyngitis;  NF-κB;  IκBα
分类号:
R 285
DOI:
10.16357/j.cnki.issn1000-5862.2020.03.08
文献标志码:
A
摘要:
以2.5%氨水喷咽部建立大鼠慢性咽炎模型,用HE染色法观察咽部病理形态学改变,用ELISA法检测血清炎症因子TNF-α、IL-1β、IL-6,用RT-qPCR测定咽部组织NF-κBp65mRNA的表达,用Western blot检测咽部组织NF-κBp65和IκBα蛋白的表达,探讨山香圆总黄酮对慢性咽炎模型大鼠 NF-κB、IκBα表达的影响.结果显示:山香圆总黄酮能明显改善模型大鼠咽部病理形态学; 与模型组比较,山香圆总黄酮各组能不同程度地下调TNF-α、IL-1β、IL-6和NF-κBp65的表达并上调IκBα的表达,其中高剂量组最显著.这表明山香圆总黄酮能通过抑制NF-κB的表达和IκBα的解离下调炎症因子TNF-α、IL-1β、IL-6的表达,从而减轻炎症,发挥抗慢性咽炎作用.
Abstract:
The rat chronic pharyngitis model is established by direct spray method of 2.5% ammonia water in rat pharyngeal mucosa.HE staining is used to observe pathological changes of pharynx.Serum inflammatory factors TNF-α,IL-1β and IL-6 are detected by ELISA.NF-κBp65 mRNA expression is detected by RT-qPCR.NF-κBp65 and IκBα protein expression is detected by Western blot.The effect of total flavonoids from Turpinia arguta Seem on the expression of NF-κB and IκBα in chronic pharyngitis model rats is investigated.The results show that total flavonoids from Turpinia arguta Seem can obviously improve the pathological morphological of pharynx tissue in rats.Compared with the model group,the total flavonoids from Turpinia arguta Seem group can down-regulate the expression of TNF-α,IL-6 and NF-κBp65,and up-regulate the expression of IκBα in different degree,which is most significant in the high dose group,which indicates that total flavonoids from Turpinia arguta Seem can restrain the expression of inflammatory factors TNF-α,IL-1β and IL-6 by inhibiting the expression of NF-κB and the dissociation of IκBα,reducing inflammation and exerting anti-chronic pharyngitis role.

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备注/Memo

备注/Memo:
收稿日期:2019-10-23
基金项目:财政部中医药公共卫生服务补助专项(财社[2017]66号),江西省教育厅科技课题(170740)和江西省卫计委中医药科研课题(2017A294)资助项目.
通信作者:黄佩蓓(1964-),女,江西南昌人,教授,主要从事中药生化研究.E-mail:2072746792@qq.com
更新日期/Last Update: 2020-06-10