[1]黄浙学,朱青青,余雨滋,等.大小鼠多种连续微量采血法的比较[J].江西师范大学学报(自然科学版),2022,(02):164-170.[doi:10.16357/j.cnki.issn1000-5862.2022.02.08]
　HUANG Zhexue,ZHU Qingqing,YU Yuzi,et al.The Comparison and Analysis of Continuous Microblood Collection on Rats and Mice[J].Journal of Jiangxi Normal University:Natural Science Edition,2022,(02):164-170.[doi:10.16357/j.cnki.issn1000-5862.2022.02.08]
点击复制

大小鼠多种连续微量采血法的比较()
分享到：

《江西师范大学学报》（自然科学版）[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2022年02期
页码:
164-170
栏目:
生命科学
出版日期:
2022-03-25

文章信息/Info

Title:
The Comparison and Analysis of Continuous Microblood Collection on Rats and Mice
文章编号:
1000-5862(2022)02-0164-07
作者:
黄浙学1; 朱青青2; 余雨滋1; 潘永良1*; 周梓欣1; 伍 爽1
1.湖州师范学院医学院,浙江省媒介生物学与病原控制重点实验室,浙江 湖州 313000; 2.双林镇中心卫生院,浙江 湖州 313012
Author(s):
HUANG Zhexue1; ZHU Qingqing2; YU Yuzi1; PAN Yongliang1*; ZHOU Zixin1; WU Shuang1
1.Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province,School of Medicine,Huzhou University,Huzhou Zhejiang 313000,China; 2.Central Health Center of Shuanglin,Huzhou Zhejiang 313012,China
关键词:
微量采血法;  研究进展;  大小鼠
Keywords:
blood micro-sampling method;  research progress;  rats and mice
分类号:
R 33
DOI:
10.16357/j.cnki.issn1000-5862.2022.02.08
文献标志码:
A
摘要:
连续微量采血可在无须处死动物的情况下追踪血样生化指标的动态变化,相比于一次性大采血可有效减少实验动物的使用量.该文查阅近年来相关资料,对大小鼠各种微量采血方式、优缺点以及注意事项进行了总结,旨在优化采血步骤、提高血样标本采集质量,从而保证实验数据的科学性和准确性,同时给予实验动物良好的福利条件.
Abstract:
Continuous micro-blood collection can track the dynamic changes of biochemical indicators of blood samples without sacrificing animals,thus it can effectively reduce the amount of experimental animals compared with one-time blood collection in a large volume.In this paper,various micro-blood collection methods in rats and mice in recent years are reviewed,and their advantages,disadvantages and precautions are discussed,aiming to optimize the blood collection steps and improve the quality of blood sample collection,so as to ensure the scientificity and accuracy of the experimental data,and provide good welfare conditions for the experimental animals.

参考文献/References:

[1] 贺争鸣,邢瑞昌,方喜业,等.我国实验动物福利立法基本原则的探讨[J].中国比较医学杂志,2005,15(2):98-100.
[2] DONCHEVA N T,PALASCA O,YARANI R,et al.Human pathways in animal models:possibilities and limitations[J].Nucleic Acids Research,2021,49(4):1859-1871.
[3] 王明丽,张晶,王耀辉,等.大鼠多次采血常用的三种方法探讨[J].实验动物科学,2013,30(4):43-45.
[4] 吴筱琴,钟声,孙锴,等.微量采血法较常规采血法的技术与优势[J].中国比较医学杂志,2018,28(3):84-90.
[5] RAJE A A,MAHAJIAN V,PATHADE V V,et al.Capillary microsampling in mice:effective way to move from sparse sampling to serial sampling in pharmacokinetics profiling[J].Xenobiotica,2020,50(6):663-669.
[6] 杨健莉,刘佳,郑志红.常用实验大小鼠采血方法及其对实验动物福利的影响[J].中国比较医学杂志,2019,29(1):90-94.
[7] WHITTAKER A L,BARKER T H.The impact of common recovery blood sampling methods,in mice(mus musculus),on well-being and sample quality:a systematic review[J].Animals-Basel,2020,10(6):989.
[8] 刘伟伟.大小鼠常见采血方法[J].中国比较医学杂志,2018,28(4):58.
[9] ZHUO Min.Nmda receptor-dependent long term hyperalgesia after tail amputation in mice[J].European Journal of Pharmacology,1998,349(2/3):211-220.
[10] 吴剑平,鞠晓云,范乃兵.不同采血方法对大鼠内分泌及血常规相关指标的影响[J].实验动物与比较医学,2014,34(5):377-380.
[11] 王洋,芦晓帆,李杭洁,等.大鼠连续尾静脉采血方法研究[J].局解手术学杂志,2015,24(5):505-507.
[12] 王晓婉,杨祎琦,王若绮,等.大鼠清醒状态下单人尾静脉注射方法研究[J].中国兽医杂志,2018,54(6):125-128.
[13] WATANABE A,WATARI R,OGAWA K,et al.Using improved serial blood sampling method of mice to study pharmacokinetics and drug-drug interaction[J].Journal of Pharmaceutical Sciences,2015,104(3):955-961.
[14] 刘永珍,王皓,杜鸣,等.单次或多次尾静脉采血对大鼠血液和血清生化指标的影响[J].实验动物与比较医学,2013,33(2):144-148.
[15] HATTORI N,TAKUMI A,SAITO K,et al.Effects of serial cervical or tail blood sampling on toxicity and toxicokinetic evaluation in rats[J].Journal of Toxicological Sciences,2020,45(10):599-609.
[16] 邹洪,闫洪涛,李军,等.大鼠实验研究中三种采血方法的应用[J].西南军医,2011,13(5):787-788.
[17] TSAI P P,SCHLICHTIG A,ZIEGLER E,et al.Effects of different blood collection methods on indicators of welfare in mice[J].Lab Animal,2015,44(8):301-310.
[18] MEYER N,KROGER M,THUMMLER J,et al.Impact of three commonly used blood sampling techniques on the welfare of laboratory mice:taking the animal's perspective[J].PloS One,2020,15(9):e0238895.
[19] DIEHL K H,HULL R,MORTON D,et al.A good practice guide to the administration of substances and removal of blood,including routes and volumes[J].Journal of Applied Toxicology,2001,21(1):15-23.
[20] SHARMA A,FISH B L,MOULDER J E,et al.Safety and blood sample volume and quality of a refined retro-orbital bleeding technique in rats using a lateral approach[J].Lab Animal,2014,43(2):63-66.
[21] GJENDAL K,KIERSGAARD M K,ABELSON K,et al.Comparison of sublingual,facial and retro-bulbar blood sampling in mice in relation to animal welfare and blood quality[J].Journal of Pharmacological and Toxicological Methods,2020,103:106680.
[22] HOLMBERG H,KIERSGAARD M K,MIKKELSEN L F,et al.Impact of blood sampling technique on blood quality and animal welfare in haemophilic mice[J].Lab Animal,2011,45(2):114-120.
[23] 吴清洪,顾为望,林继红,等.一种大鼠采血方法介绍[J].动物医学进展,2004,25(1):133.
[24] 姜波涛.探讨一种新的大鼠取血方法:舌下静脉采血[J].临床和实验医学杂志,2011,10(5):384.
[25] 安琪,傅龙龙,张长勇,等.改良大鼠舌下静脉采血法的应用[J].局解手术学杂志,2019,28(3):250-252.
[26] 马征,刘秀英,黄凰.两种采血方法对大鼠生化和血相指标的影响研究[J].湖南师范大学学报(医学版),2012,9(2):44-46.
[27] 杨萍,赵月,杨帆,等.改良大鼠颈静脉采血法[J].局解手术学杂志,2017,26(3):221-223.
[28] 黄光琳,郑军.大鼠颈静脉采血体会[J].局解手术学杂志,2011,20(3):330.
[29] 王敏康,王晓燕,张田,等.一种用于大鼠的无麻醉可连续的采血技术[J].解剖学杂志,1999,22(1):94-95.
[30] 徐新建,温浩,王喜艳,等.一种大鼠颈外静脉持久性置管术[J].中华实验外科杂志,2003,20(5):478.
[31] 邝荔香,陈敏珠.药理学动物实验插管扎口方式与固定效果探讨[J].安徽医药,2007,11(3):278.
[32] PARK A Y,PLOTSKY P M,PHAM T D,et al.Blood collection in unstressed,conscious,and freely moving mice through implantation of catheters in the jugular vein:a new simplified protocol[J].Physiol Rep,2018,6(21):e13904.
[33] 李卫国,罗艳蕊,翟心慧,等.大鼠连续定时采血新方法:股动脉插管法[J].生物学杂志,2001,18(6):33.
[34] 李静.实验大鼠常规采血法及改进研究进展[J].当代畜牧,2017,30(10):48-50.
[35] GOTTDENKER N L,JACOBSON E R.Effect of venipuncture sites on hematologic and clinical biochemical values in desert tortoises(gopherus agassizii)[J].Americal Journal of Veterinary Research,1995,56(1):19-21.
[36] ABATAN O I,WELCH K B,NEMZEK J A.Evaluation of saphenous venipuncture and modified tail-clip blood collection in mice[J].Journal of the American Association for Laboratory Animal Science,2008,47(3):8-15.
[37] HOFF J.Methods of blood collection in the mouse[J].Lab Animal,2000,29(10):47-53.
[38] 张东淑,林少霞,韩强,等.小鼠颌下静脉丛采血法改良[J].毒理学杂志,2018,32(6):499-500.
[39] 台雪姣,郭兴荣,罗超,等.小鼠颌下采血法改良[J].四川动物,2017,36(2):211-214.
[40] 金丹丹,张劲松,丁航,等.蒙古长爪沙鼠颌下静脉丛真空采血法探讨[J].实验动物科学,2014,31(6):40-42.
[41] 张帅帅,李天寿,藏美蓉,等.鼠面颊采血方法探究[J].中华医学杂志,2016,96(8):655-657.
[42] JO E J,BAE E,YOON J H,et al.Comparison of murine retroorbital plexus and facial vein blood collection to mitigate animal ethics issues[J].Laboratory Animal Research,2021,37(1):12.
[43] GOLDE W T,GOLLOBIN P,RODRIGUEZ L L.A rapid,simple,and humane method for submandibular bleeding of mice using a lancet[J].Lab Animal,2005,34(10):39-43.
[44] VOIGT C,KLOCKNER P,TOUMA C,et al.Hormonal stress response of laboratory mice to conventional and minimally invasive bleeding techniques[J].Animal Welfare,2013,22(4):449-455.
[45] 谭剑斌,陈瑞仪,胡帅尔.SD大鼠麻醉后生化指标变化[J].中国比较医学杂志,2008(1):43-45.
[46] 陈秋生,梁泽华,林春燕.水合氯醛、异丙酚、氯胺酮麻醉对小鼠血液学指标的影响[J].实验动物科学,2013,30(3):16-18.
[47] 尤国兴,王瑛,王波,等.麻醉方法对大鼠生理、血气及血液流变学的影响[J].中国输血杂志,2013,26(5):417-420.

相似文献/References:

[1]罗 津*,刘 丽*,俎晓芳,等.近20年鄱阳湖研究追踪——基于CNKI和CiteSpace文献计量法[J].江西师范大学学报(自然科学版),2019,(02):206.[doi:10.16357/j.cnki.issn1000-5862.2019.02.15]
　LUO Jin*,LIU Li*,ZU Xiaofang,et al.The Research on Poyang Lake Literature Published in Recent 20 Years——Based on CNKI and CiteSpace Bibliometric Method[J].Journal of Jiangxi Normal University:Natural Science Edition,2019,(02):206.[doi:10.16357/j.cnki.issn1000-5862.2019.02.15]
[2]钟业喜,邵海雁,徐晨璐,等.基于文献计量分析的流域山水林田湖草生命共同体研究进展与展望[J].江西师范大学学报(自然科学版),2020,(01):95.[doi:10.16357/j.cnki.issn1000-5862.2020.01.16]
　ZHONG Yexi,SHAO Haiyan,XU Chenlu,et al.The Research Progress and Prospect of Life Community of Mountain,River,Forest,Farmland,Lake and Grasslandin Watershed Based on Bibliometric Analysis[J].Journal of Jiangxi Normal University:Natural Science Edition,2020,(02):95.[doi:10.16357/j.cnki.issn1000-5862.2020.01.16]

备注/Memo

备注/Memo:
收稿日期:2021-10-25
基金项目:国家自然科学基金(31672295)和国家级大学生创新创业训练计划(202110347050)资助项目.
通信作者:潘永良(1979—),男,浙江诸暨人,教授,博士,主要从事行为神经内分泌研究.E-mail:02402@zjhu.edu.cn
更新日期/Last Update: 2022-03-25